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Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species.
Mutat Res. 2007 Jul 10; 631(1):36-43.MR

Abstract

Numerous spills and leakages involving petroleum and its derivatives have recently occurred in Brazilian rivers. Considering the lack of information regarding the genotoxic response of neotropical fish to these events and the predominance of information regarding saltwater fish, which offers no genuine comparisons, the present work aimed to evaluate the genotoxicity and mutagenicity of the diesel water soluble fraction (DWSF) on the neotropical fish Prochilodus lineatus under acute (6, 24 and 96h) and subchronic (15 days) exposures, using the comet (SCGE) and micronucleus assays. The results indicated genotoxic and mutagenic damage in erythrocytes of P. lineatus exposed to DWSF. Comet scores for fish exposed to DWSF in all experimental periods were significantly higher than the respective negative control groups (fish exposed to clean water for the same period). The relative frequencies of micronucleated erythrocytes for P. lineatus exposed to DWSF under acute and subchronic treatment were also significantly higher than their respective negative controls. Taken together these results showed that acute and subchronic exposures to DWSF produce mutagenic and genotoxic effects on the blood cells of P. lineatus and that the combination of comet and micronucleus assays proved to be both suitable and useful in the evaluation of the genotoxicity of diesel oil due to their complementary action.

Authors+Show Affiliations

Departamento de Biologia Geral, Universidade Estadual de Londrina (UEL), Paraná, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17509929

Citation

Vanzella, T P., et al. "Genotoxic and Mutagenic Effects of Diesel Oil Water Soluble Fraction On a Neotropical Fish Species." Mutation Research, vol. 631, no. 1, 2007, pp. 36-43.
Vanzella TP, Martinez CB, Cólus IM. Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species. Mutat Res. 2007;631(1):36-43.
Vanzella, T. P., Martinez, C. B., & Cólus, I. M. (2007). Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species. Mutation Research, 631(1), 36-43.
Vanzella TP, Martinez CB, Cólus IM. Genotoxic and Mutagenic Effects of Diesel Oil Water Soluble Fraction On a Neotropical Fish Species. Mutat Res. 2007 Jul 10;631(1):36-43. PubMed PMID: 17509929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic and mutagenic effects of diesel oil water soluble fraction on a neotropical fish species. AU - Vanzella,T P, AU - Martinez,C B R, AU - Cólus,I M S, Y1 - 2007/04/18/ PY - 2006/12/06/received PY - 2007/04/10/revised PY - 2007/04/11/accepted PY - 2007/5/19/pubmed PY - 2007/8/11/medline PY - 2007/5/19/entrez SP - 36 EP - 43 JF - Mutation research JO - Mutat Res VL - 631 IS - 1 N2 - Numerous spills and leakages involving petroleum and its derivatives have recently occurred in Brazilian rivers. Considering the lack of information regarding the genotoxic response of neotropical fish to these events and the predominance of information regarding saltwater fish, which offers no genuine comparisons, the present work aimed to evaluate the genotoxicity and mutagenicity of the diesel water soluble fraction (DWSF) on the neotropical fish Prochilodus lineatus under acute (6, 24 and 96h) and subchronic (15 days) exposures, using the comet (SCGE) and micronucleus assays. The results indicated genotoxic and mutagenic damage in erythrocytes of P. lineatus exposed to DWSF. Comet scores for fish exposed to DWSF in all experimental periods were significantly higher than the respective negative control groups (fish exposed to clean water for the same period). The relative frequencies of micronucleated erythrocytes for P. lineatus exposed to DWSF under acute and subchronic treatment were also significantly higher than their respective negative controls. Taken together these results showed that acute and subchronic exposures to DWSF produce mutagenic and genotoxic effects on the blood cells of P. lineatus and that the combination of comet and micronucleus assays proved to be both suitable and useful in the evaluation of the genotoxicity of diesel oil due to their complementary action. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/17509929/Genotoxic_and_mutagenic_effects_of_diesel_oil_water_soluble_fraction_on_a_neotropical_fish_species_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(07)00102-7 DB - PRIME DP - Unbound Medicine ER -