Tags

Type your tag names separated by a space and hit enter

Biomonitoring of cyanobacterial blooms in Polish water reservoir and the cytotoxicity and genotoxicity of selected cyanobacterial extracts.
Int J Occup Med Environ Health. 2007; 20(1):48-65.IJ

Abstract

OBJECTIVES

Water pollution with toxic cyanobacterial blooms is a worldwide problem. Cyanobacteria species that mainly produce microcystins predominate in Polish water reservoirs.

MATERIALS AND METHODS

In our study, cyanobacterial blooms were monitored during summer of 2004 in the Sulejów reservoir. The concentration of microcystins in water and cyanobacterial cells were determined using liquid chromatography and immunobiotests, while the biological activity of microcystic cyanobacterial extracts was assessed using bacterial tests (SOS Chromotest, UMU test), the comet assay and micronucleus test with human lymphocytes.

RESULTS

It was revealed that cyanobacterial bloom was most intensive in mid August and lasted until the end of September. Microcystis aeruginosa and Aphanizomenon flos-aquae dominated in the blooms. The highest concentration of microcystins in cyanobacterial cells was also observed at that time. The concentration of microcystins in water did not exceed 1 microg/l. All cyanobacterial extracts showed weak genotoxicity only for Escherichia coli PQ37. The cyanobacterial extracts prepared at the beginning of September were most toxic to human lymphocytes, the effective microcystin extracts (EC50) concentration was about two or three times lower compared to the other extracts. The level of DNA damage in lymphocytes after short exposure to microcystic extracts (3 and 6 h) was significantly higher than respective levels after longer exposure. The microcystins of cyanobacterial blooms induced a slight increase in micronuclei frequencies in human lymphocytes.

CONCLUSION

Phytoplankton biomass and the genotoxicity of massive cyanobacterial blooms should be assessed for eucariotic cells in the Sulejów reservoir to avoid the hazard induced by cyanobacterial blooms.

Authors+Show Affiliations

Department of Toxicology and Carcinogenesis, Nofer Institute of Occupational Medicine, św. Teresy 8, 91-348 Łódź, Poland. jadwigap@imp.lodz.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17509970

Citation

Palus, Jadwiga, et al. "Biomonitoring of Cyanobacterial Blooms in Polish Water Reservoir and the Cytotoxicity and Genotoxicity of Selected Cyanobacterial Extracts." International Journal of Occupational Medicine and Environmental Health, vol. 20, no. 1, 2007, pp. 48-65.
Palus J, Dziubałtowska E, Stańczyk M, et al. Biomonitoring of cyanobacterial blooms in Polish water reservoir and the cytotoxicity and genotoxicity of selected cyanobacterial extracts. Int J Occup Med Environ Health. 2007;20(1):48-65.
Palus, J., Dziubałtowska, E., Stańczyk, M., Lewińska, D., Mankiewicz-Boczek, J., Izydorczyk, K., Bonisławska, A., Jurczak, T., Zalewski, M., & Wasowicz, W. (2007). Biomonitoring of cyanobacterial blooms in Polish water reservoir and the cytotoxicity and genotoxicity of selected cyanobacterial extracts. International Journal of Occupational Medicine and Environmental Health, 20(1), 48-65.
Palus J, et al. Biomonitoring of Cyanobacterial Blooms in Polish Water Reservoir and the Cytotoxicity and Genotoxicity of Selected Cyanobacterial Extracts. Int J Occup Med Environ Health. 2007;20(1):48-65. PubMed PMID: 17509970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomonitoring of cyanobacterial blooms in Polish water reservoir and the cytotoxicity and genotoxicity of selected cyanobacterial extracts. AU - Palus,Jadwiga, AU - Dziubałtowska,Elzbieta, AU - Stańczyk,Małgorzata, AU - Lewińska,Dobrosława, AU - Mankiewicz-Boczek,Joanna, AU - Izydorczyk,Katarzyna, AU - Bonisławska,Anita, AU - Jurczak,Tomasz, AU - Zalewski,Maciej, AU - Wasowicz,Wojciech, PY - 2007/5/19/pubmed PY - 2007/9/18/medline PY - 2007/5/19/entrez SP - 48 EP - 65 JF - International journal of occupational medicine and environmental health JO - Int J Occup Med Environ Health VL - 20 IS - 1 N2 - OBJECTIVES: Water pollution with toxic cyanobacterial blooms is a worldwide problem. Cyanobacteria species that mainly produce microcystins predominate in Polish water reservoirs. MATERIALS AND METHODS: In our study, cyanobacterial blooms were monitored during summer of 2004 in the Sulejów reservoir. The concentration of microcystins in water and cyanobacterial cells were determined using liquid chromatography and immunobiotests, while the biological activity of microcystic cyanobacterial extracts was assessed using bacterial tests (SOS Chromotest, UMU test), the comet assay and micronucleus test with human lymphocytes. RESULTS: It was revealed that cyanobacterial bloom was most intensive in mid August and lasted until the end of September. Microcystis aeruginosa and Aphanizomenon flos-aquae dominated in the blooms. The highest concentration of microcystins in cyanobacterial cells was also observed at that time. The concentration of microcystins in water did not exceed 1 microg/l. All cyanobacterial extracts showed weak genotoxicity only for Escherichia coli PQ37. The cyanobacterial extracts prepared at the beginning of September were most toxic to human lymphocytes, the effective microcystin extracts (EC50) concentration was about two or three times lower compared to the other extracts. The level of DNA damage in lymphocytes after short exposure to microcystic extracts (3 and 6 h) was significantly higher than respective levels after longer exposure. The microcystins of cyanobacterial blooms induced a slight increase in micronuclei frequencies in human lymphocytes. CONCLUSION: Phytoplankton biomass and the genotoxicity of massive cyanobacterial blooms should be assessed for eucariotic cells in the Sulejów reservoir to avoid the hazard induced by cyanobacterial blooms. SN - 1232-1087 UR - https://www.unboundmedicine.com/medline/citation/17509970/Biomonitoring_of_cyanobacterial_blooms_in_Polish_water_reservoir_and_the_cytotoxicity_and_genotoxicity_of_selected_cyanobacterial_extracts_ DB - PRIME DP - Unbound Medicine ER -