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High-resolution analysis of CpG methylation and in vivo protein-DNA interactions at the alternative Epstein-Barr virus latency promoters Qp and Cp in the nasopharyngeal carcinoma cell line C666-1.
Virus Genes 2007; 35(2):195-202VG

Abstract

Transcripts for the Epstein-Barr virus (EBV) encoded nuclear antigens (EBNAs) are initiated at alternative promoters (Wp, Cp, for EBNA 1-6 transcripts and Qp, for EBNA 1 transcripts only) located in the BamHI W, C or Q fragment of the viral genome. To understand the host-cell dependent expression of EBNAs in EBV-associated tumors (lymphomas and carcinomas) and in vitro transformed cell lines, it is necessary to analyse the regulatory mechanisms governing the activity of the alternative promoters of EBNA transcripts. Such studies focused mainly on lymphoid cell lines carrying latent EBV genomes, due to the lack of EBV-associated carcinoma cell lines maintaining latent EBV genomes during cultivation in tissue culture. We took advantage of the unique nasopharyngeal carcinoma cell line, C666-1, harboring EBV genomes, and undertook a detailed analysis of CpG methylation patterns and in vivo protein-DNA interactions at the latency promoters Qp and Cp. We found that the active, unmethylated Qp was marked with strong footprints of cellular transcription factors and the viral protein EBNA 1. In contrast, we could not detect binding of relevant transcription factors to the methylated, silent Cp. We concluded that the epigenetic marks at Qp and Cp in C666-1 cells of epithelial origin resemble those of group I Burkitt's lymphoma cell lines.

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    Authors+Show Affiliations

    Bakos A
    Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, 1529 Budapest, Hungary.
    Banati F
    No affiliation info available
    Koroknai A
    No affiliation info available
    Takacs M
    No affiliation info available
    Salamon D
    No affiliation info available
    Minarovits-Kormuta S
    No affiliation info available
    Schwarzmann F
    No affiliation info available
    Wolf H
    No affiliation info available
    Niller HH
    No affiliation info available
    Minarovits J
    No affiliation info available

    MeSH

    Base SequenceCarcinomaCell Line, TumorCpG IslandsDNA MethylationDNA, ViralDNA-Binding ProteinsHerpesvirus 4, HumanHumansMolecular Sequence DataNasopharyngeal NeoplasmsPromoter Regions, GeneticTranscriptional ActivationVirus Latency

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17510783

    Citation

    Bakos, Agnes, et al. "High-resolution Analysis of CpG Methylation and in Vivo protein-DNA Interactions at the Alternative Epstein-Barr Virus Latency Promoters Qp and Cp in the Nasopharyngeal Carcinoma Cell Line C666-1." Virus Genes, vol. 35, no. 2, 2007, pp. 195-202.
    Bakos A, Banati F, Koroknai A, et al. High-resolution analysis of CpG methylation and in vivo protein-DNA interactions at the alternative Epstein-Barr virus latency promoters Qp and Cp in the nasopharyngeal carcinoma cell line C666-1. Virus Genes. 2007;35(2):195-202.
    Bakos, A., Banati, F., Koroknai, A., Takacs, M., Salamon, D., Minarovits-Kormuta, S., ... Minarovits, J. (2007). High-resolution analysis of CpG methylation and in vivo protein-DNA interactions at the alternative Epstein-Barr virus latency promoters Qp and Cp in the nasopharyngeal carcinoma cell line C666-1. Virus Genes, 35(2), pp. 195-202.
    Bakos A, et al. High-resolution Analysis of CpG Methylation and in Vivo protein-DNA Interactions at the Alternative Epstein-Barr Virus Latency Promoters Qp and Cp in the Nasopharyngeal Carcinoma Cell Line C666-1. Virus Genes. 2007;35(2):195-202. PubMed PMID: 17510783.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - High-resolution analysis of CpG methylation and in vivo protein-DNA interactions at the alternative Epstein-Barr virus latency promoters Qp and Cp in the nasopharyngeal carcinoma cell line C666-1. AU - Bakos,Agnes, AU - Banati,Ferenc, AU - Koroknai,Anita, AU - Takacs,Maria, AU - Salamon,Daniel, AU - Minarovits-Kormuta,Susanna, AU - Schwarzmann,Fritz, AU - Wolf,Hans, AU - Niller,Hans Helmut, AU - Minarovits,Janos, Y1 - 2007/05/18/ PY - 2006/11/28/received PY - 2007/03/08/accepted PY - 2007/5/19/pubmed PY - 2007/10/17/medline PY - 2007/5/19/entrez SP - 195 EP - 202 JF - Virus genes JO - Virus Genes VL - 35 IS - 2 N2 - Transcripts for the Epstein-Barr virus (EBV) encoded nuclear antigens (EBNAs) are initiated at alternative promoters (Wp, Cp, for EBNA 1-6 transcripts and Qp, for EBNA 1 transcripts only) located in the BamHI W, C or Q fragment of the viral genome. To understand the host-cell dependent expression of EBNAs in EBV-associated tumors (lymphomas and carcinomas) and in vitro transformed cell lines, it is necessary to analyse the regulatory mechanisms governing the activity of the alternative promoters of EBNA transcripts. Such studies focused mainly on lymphoid cell lines carrying latent EBV genomes, due to the lack of EBV-associated carcinoma cell lines maintaining latent EBV genomes during cultivation in tissue culture. We took advantage of the unique nasopharyngeal carcinoma cell line, C666-1, harboring EBV genomes, and undertook a detailed analysis of CpG methylation patterns and in vivo protein-DNA interactions at the latency promoters Qp and Cp. We found that the active, unmethylated Qp was marked with strong footprints of cellular transcription factors and the viral protein EBNA 1. In contrast, we could not detect binding of relevant transcription factors to the methylated, silent Cp. We concluded that the epigenetic marks at Qp and Cp in C666-1 cells of epithelial origin resemble those of group I Burkitt's lymphoma cell lines. SN - 0920-8569 UR - https://www.unboundmedicine.com/medline/citation/17510783/High_resolution_analysis_of_CpG_methylation_and_in_vivo_protein_DNA_interactions_at_the_alternative_Epstein_Barr_virus_latency_promoters_Qp_and_Cp_in_the_nasopharyngeal_carcinoma_cell_line_C666_1_ L2 - https://doi.org/10.1007/s11262-007-0095-y DB - PRIME DP - Unbound Medicine ER -