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Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry.
Rapid Commun Mass Spectrom. 2007; 21(12):1855-65.RC

Abstract

High-performance liquid chromatography-diode array detection (HPLC-DAD), electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) and electrospray ionization quadrupole ion trap mass spectrometry (HPLC-ESI-MSn) were used for the isolation, identification and structural analysis of water-soluble phenolic and nonpolar diterpenoid constituents in Dan-shen (Radix Salvia miltiorrhizae) which was prepared by sonication in 70% methanol. Mass spectra were obtained by ESI-TOF-MS and electrospray ionization quadrupole ion trap mass spectrometry (ESI-QIT-MS). A formula database of known constituents in Dan-shen was established and most constituents were rapidly identified by HPLC-DAD/ESI-TOF-MS by matching their accurate molecular masses with the formulae of the compounds in the database. Compounds with the same molecular formula could not be differentiated by TOF-MS; however, QIT-MS could differentiate those compounds and elucidate their structures based on their characteristic fragmentation. HPLC-DAD, HPLC/ESI-TOF-MS and HPLC/ESI-MSn provided complementary information for the identification of the constituents in Dan-shen. Forty constituents were identified in 30 min based on their positive and negative ion ESI mass spectra and liquid chromatographic information. Thus the method described is useful for the rapid analysis of multiple constituents in Dan-shen.

Authors+Show Affiliations

Zhu Z
Department of Drug Analysis, School of Pharmacy, Second Military Medical University, and Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200433, China.
Zhang H
No affiliation info available
Zhao L
No affiliation info available
Dong X
No affiliation info available
Li X
No affiliation info available
Chai Y
No affiliation info available
Zhang G
No affiliation info available

MeSH

Chromatography, High Pressure LiquidDiterpenesMolecular StructurePhenolsSalvia miltiorrhizaSpectrometry, Mass, Electrospray IonizationTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17510941

Citation

Zhu, Zhenyu, et al. "Rapid Separation and Identification of Phenolic and Diterpenoid Constituents From Radix Salvia Miltiorrhizae By High-performance Liquid Chromatography Diode-array Detection, Electrospray Ionization Time-of-flight Mass Spectrometry and Electrospray Ionization Quadrupole Ion Trap Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 12, 2007, pp. 1855-65.
Zhu Z, Zhang H, Zhao L, et al. Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry. Rapid Commun Mass Spectrom. 2007;21(12):1855-65.
Zhu, Z., Zhang, H., Zhao, L., Dong, X., Li, X., Chai, Y., & Zhang, G. (2007). Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 21(12), 1855-65.
Zhu Z, et al. Rapid Separation and Identification of Phenolic and Diterpenoid Constituents From Radix Salvia Miltiorrhizae By High-performance Liquid Chromatography Diode-array Detection, Electrospray Ionization Time-of-flight Mass Spectrometry and Electrospray Ionization Quadrupole Ion Trap Mass Spectrometry. Rapid Commun Mass Spectrom. 2007;21(12):1855-65. PubMed PMID: 17510941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry. AU - Zhu,Zhenyu, AU - Zhang,Hai, AU - Zhao,Liang, AU - Dong,Xin, AU - Li,Xiang, AU - Chai,Yifeng, AU - Zhang,Guoqing, PY - 2007/5/19/pubmed PY - 2007/8/8/medline PY - 2007/5/19/entrez SP - 1855 EP - 65 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 12 N2 - High-performance liquid chromatography-diode array detection (HPLC-DAD), electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) and electrospray ionization quadrupole ion trap mass spectrometry (HPLC-ESI-MSn) were used for the isolation, identification and structural analysis of water-soluble phenolic and nonpolar diterpenoid constituents in Dan-shen (Radix Salvia miltiorrhizae) which was prepared by sonication in 70% methanol. Mass spectra were obtained by ESI-TOF-MS and electrospray ionization quadrupole ion trap mass spectrometry (ESI-QIT-MS). A formula database of known constituents in Dan-shen was established and most constituents were rapidly identified by HPLC-DAD/ESI-TOF-MS by matching their accurate molecular masses with the formulae of the compounds in the database. Compounds with the same molecular formula could not be differentiated by TOF-MS; however, QIT-MS could differentiate those compounds and elucidate their structures based on their characteristic fragmentation. HPLC-DAD, HPLC/ESI-TOF-MS and HPLC/ESI-MSn provided complementary information for the identification of the constituents in Dan-shen. Forty constituents were identified in 30 min based on their positive and negative ion ESI mass spectra and liquid chromatographic information. Thus the method described is useful for the rapid analysis of multiple constituents in Dan-shen. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17510941/Rapid_separation_and_identification_of_phenolic_and_diterpenoid_constituents_from_Radix_Salvia_miltiorrhizae_by_high_performance_liquid_chromatography_diode_array_detection_electrospray_ionization_time_of_flight_mass_spectrometry_and_electrospray_ionization_quadrupole_ion_trap_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3023 DB - PRIME DP - Unbound Medicine ER -
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