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Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets.
Transpl Infect Dis. 2008 Apr; 10(2):99-105.TI

Abstract

Current immunosuppression protocols to prevent lung transplant rejection reduce pro-inflammatory and T-helper type 1 (Th1) cytokines. However, Th1 T-cell pro-inflammatory cytokine production is important in host defense against bacterial infection in the lungs. Excessive immunosuppression of Th1 T-cell pro-inflammatory cytokines leaves patients susceptible to infection. To investigate whether pulmonary infection in lung transplant recipients is associated with reduced Th1 T-cell pro-inflammatory cytokines, whole blood and bronchoalveolar lavage (BAL) fluid from 13 stable lung transplant patients with 'culture-negative' BAL and 13 patients with 'culture-positive' BAL was stimulated in vitro, and cytokine production by CD8+ and CD4+ T-cell subsets was determined using multiparameter flow cytometry. In BAL samples, there was a significant decrease in interleukin-2 (IL2) in CD3+ T cells and tumor necrosis factor-alpha (TNF-alpha) in CD8+ T cells (but not CD4+) in 'culture-positive' compared with 'culture-negative' transplant patients. There was no difference in blood Th1 T-cell cytokines between 'culture-positive' compared with 'culture-negative' transplant patients. A decrease in Th1 cytokines IL-2 and TNF-alpha in BAL T-cell subsets is associated with isolation of potentially pathogenic organisms in the lungs in stable lung transplant patients. Excessive immunosuppression of these Th1 T-cell pro-inflammatory cytokines in stable transplant patients may leave them susceptible to infection. Modifying immunosuppression by monitoring intracellular Th1 pro-inflammatory cytokines in BAL T cells may help to improve morbidity and infection rates in stable lung transplant patients.

Authors+Show Affiliations

Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, South Australia, Australia. greg.hodge@cywhs.sa.gov.auNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17511818

Citation

Hodge, G, et al. "Airway Infection in Stable Lung Transplant Patients Is Associated With Decreased Intracellular T-helper Type 1 Pro-inflammatory Cytokines in Bronchoalveolar Lavage T-cell Subsets." Transplant Infectious Disease : an Official Journal of the Transplantation Society, vol. 10, no. 2, 2008, pp. 99-105.
Hodge G, Hodge S, Reynolds PN, et al. Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets. Transpl Infect Dis. 2008;10(2):99-105.
Hodge, G., Hodge, S., Reynolds, P. N., & Holmes, M. (2008). Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets. Transplant Infectious Disease : an Official Journal of the Transplantation Society, 10(2), 99-105.
Hodge G, et al. Airway Infection in Stable Lung Transplant Patients Is Associated With Decreased Intracellular T-helper Type 1 Pro-inflammatory Cytokines in Bronchoalveolar Lavage T-cell Subsets. Transpl Infect Dis. 2008;10(2):99-105. PubMed PMID: 17511818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets. AU - Hodge,G, AU - Hodge,S, AU - Reynolds,P N, AU - Holmes,M, Y1 - 2007/05/19/ PY - 2007/5/22/pubmed PY - 2008/5/17/medline PY - 2007/5/22/entrez SP - 99 EP - 105 JF - Transplant infectious disease : an official journal of the Transplantation Society JO - Transpl Infect Dis VL - 10 IS - 2 N2 - Current immunosuppression protocols to prevent lung transplant rejection reduce pro-inflammatory and T-helper type 1 (Th1) cytokines. However, Th1 T-cell pro-inflammatory cytokine production is important in host defense against bacterial infection in the lungs. Excessive immunosuppression of Th1 T-cell pro-inflammatory cytokines leaves patients susceptible to infection. To investigate whether pulmonary infection in lung transplant recipients is associated with reduced Th1 T-cell pro-inflammatory cytokines, whole blood and bronchoalveolar lavage (BAL) fluid from 13 stable lung transplant patients with 'culture-negative' BAL and 13 patients with 'culture-positive' BAL was stimulated in vitro, and cytokine production by CD8+ and CD4+ T-cell subsets was determined using multiparameter flow cytometry. In BAL samples, there was a significant decrease in interleukin-2 (IL2) in CD3+ T cells and tumor necrosis factor-alpha (TNF-alpha) in CD8+ T cells (but not CD4+) in 'culture-positive' compared with 'culture-negative' transplant patients. There was no difference in blood Th1 T-cell cytokines between 'culture-positive' compared with 'culture-negative' transplant patients. A decrease in Th1 cytokines IL-2 and TNF-alpha in BAL T-cell subsets is associated with isolation of potentially pathogenic organisms in the lungs in stable lung transplant patients. Excessive immunosuppression of these Th1 T-cell pro-inflammatory cytokines in stable transplant patients may leave them susceptible to infection. Modifying immunosuppression by monitoring intracellular Th1 pro-inflammatory cytokines in BAL T cells may help to improve morbidity and infection rates in stable lung transplant patients. SN - 1399-3062 UR - https://www.unboundmedicine.com/medline/citation/17511818/Airway_infection_in_stable_lung_transplant_patients_is_associated_with_decreased_intracellular_T_helper_type_1_pro_inflammatory_cytokines_in_bronchoalveolar_lavage_T_cell_subsets_ L2 - https://doi.org/10.1111/j.1399-3062.2007.00236.x DB - PRIME DP - Unbound Medicine ER -