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Characterization of the fraction components using 1D TOCSY and 1D ROESY experiments. Four new spirostane saponins from Agave brittoniana Trel. spp. Brachypus.
Magn Reson Chem. 2007 Jul; 45(7):615-20.MR

Abstract

A careful NMR analysis, especially 1D TOCSY and 1D ROESY, of two refined saponin fractions allowed us to determine the structures of four new saponins from a polar extract of the Agave brittoniana Trel. spp. Brachypus leaves. A full assignment of the 1H and 13C spectral data for these new saponins, agabrittonosides A-D (1-4), and one previously known saponin, karatavioside A (5) is reported. Their structures were established using a combination of 1D and 2D (1H, 1H-COSY, TOCSY, ROESY, g-HSQC, g-HMBC and g-HSQC-TOCSY) NMR techniques and ESI-MS. Moreover, the work represents a new approach to structural elucidation of saponins in refined fractions by NMR investigations.

Authors+Show Affiliations

Macías FA
Grupo de Alelopatía, Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, C/República Saharaui, s/n, 11510 Puerto Real, Cádiz, Spain. famacias@uca.es
Guerra JO
No affiliation info available
Simonet AM
No affiliation info available
Nogueiras CM
No affiliation info available

MeSH

AgaveMagnetic Resonance SpectroscopyMolecular StructurePlant ExtractsPlants, MedicinalSaponinsSpirostans

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17516586

Citation

Macías, Francisco A., et al. "Characterization of the Fraction Components Using 1D TOCSY and 1D ROESY Experiments. Four New Spirostane Saponins From Agave Brittoniana Trel. Spp. Brachypus." Magnetic Resonance in Chemistry : MRC, vol. 45, no. 7, 2007, pp. 615-20.
Macías FA, Guerra JO, Simonet AM, et al. Characterization of the fraction components using 1D TOCSY and 1D ROESY experiments. Four new spirostane saponins from Agave brittoniana Trel. spp. Brachypus. Magn Reson Chem. 2007;45(7):615-20.
Macías, F. A., Guerra, J. O., Simonet, A. M., & Nogueiras, C. M. (2007). Characterization of the fraction components using 1D TOCSY and 1D ROESY experiments. Four new spirostane saponins from Agave brittoniana Trel. spp. Brachypus. Magnetic Resonance in Chemistry : MRC, 45(7), 615-20.
Macías FA, et al. Characterization of the Fraction Components Using 1D TOCSY and 1D ROESY Experiments. Four New Spirostane Saponins From Agave Brittoniana Trel. Spp. Brachypus. Magn Reson Chem. 2007;45(7):615-20. PubMed PMID: 17516586.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the fraction components using 1D TOCSY and 1D ROESY experiments. Four new spirostane saponins from Agave brittoniana Trel. spp. Brachypus. AU - Macías,Francisco A, AU - Guerra,José O, AU - Simonet,Ana M, AU - Nogueiras,Clara M, PY - 2007/5/23/pubmed PY - 2007/9/20/medline PY - 2007/5/23/entrez SP - 615 EP - 20 JF - Magnetic resonance in chemistry : MRC JO - Magn Reson Chem VL - 45 IS - 7 N2 - A careful NMR analysis, especially 1D TOCSY and 1D ROESY, of two refined saponin fractions allowed us to determine the structures of four new saponins from a polar extract of the Agave brittoniana Trel. spp. Brachypus leaves. A full assignment of the 1H and 13C spectral data for these new saponins, agabrittonosides A-D (1-4), and one previously known saponin, karatavioside A (5) is reported. Their structures were established using a combination of 1D and 2D (1H, 1H-COSY, TOCSY, ROESY, g-HSQC, g-HMBC and g-HSQC-TOCSY) NMR techniques and ESI-MS. Moreover, the work represents a new approach to structural elucidation of saponins in refined fractions by NMR investigations. SN - 0749-1581 UR - https://www.unboundmedicine.com/medline/citation/17516586/Characterization_of_the_fraction_components_using_1D_TOCSY_and_1D_ROESY_experiments__Four_new_spirostane_saponins_from_Agave_brittoniana_Trel__spp__Brachypus_ DB - PRIME DP - Unbound Medicine ER -