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Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma.
Clin Exp Allergy. 2007 Jun; 37(6):872-9.CE

Abstract

BACKGROUND

Allergic conditions in different organs share many similarities in their inflammatory response. Vernal keratoconjunctivitis (VKC), asthma and nasal polyps exhibit several similar, but site-specific mucosal structural changes. The aim of the study was to investigate whether matrix metalloproteases contribute to different tissue remodelling aspects in different organs.

METHODS

Mucosal biopsies were obtained from conjunctiva of healthy donors, tarsal conjunctiva of vernal patients, bronchi of non-asthmatic subjects, bronchi of mild stable asthmatic patients, nasal mucosa of non-allergic donors and nasal polyps of allergic patients. Distribution of metalloprotease-1, -3, -9, -13, tissue inhibitor of metalloproteases-1, collagens I and III and the presence of eosinophils and CD4+ cells were evaluated by immunohistochemistry.

RESULTS

Collagens were highly diffuse in the giant papillae of VKC and in nasal polyps, and yet less increased in the subepithelium of asthmatic patients. Immunostaining for metalloprotease-1, -3, -9 and -13 was significantly higher in VKC compared with normal conjunctiva. Metalloprotease-9 staining was higher in the stroma of polyps vs. normal nasal mucosa, and only metalloprotease-13 was significantly more expressed in asthmatic vs. non-asthmatic subjects. Metalloprotease-9 immunostaining was more intense in vernal compared with other tissues. In all pathological tissues, metalloprotease-9-positive staining was in association with eosinophils and CD4+ cells.

CONCLUSIONS

Expression of metalloproteases may play an important role in inducing the structural changes seen in VKC, nasal polyps and asthma. Tissue remodelling and gelatinase immunoexpression was more dramatic in giant papillae of vernal patients compared with other tissue sites of chronic allergic inflammation.

Authors+Show Affiliations

Ophthalmology Unit, Department of Neuroscience, University of Padua, Italy. andrea.leonardi@unipd.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17517101

Citation

Leonardi, A, et al. "Matrix Metalloproteases in Vernal Keratoconjunctivitis, Nasal Polyps and Allergic Asthma." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 37, no. 6, 2007, pp. 872-9.
Leonardi A, Brun P, Di Stefano A, et al. Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma. Clin Exp Allergy. 2007;37(6):872-9.
Leonardi, A., Brun, P., Di Stefano, A., Motterle, L., & Abatangelo, G. (2007). Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 37(6), 872-9.
Leonardi A, et al. Matrix Metalloproteases in Vernal Keratoconjunctivitis, Nasal Polyps and Allergic Asthma. Clin Exp Allergy. 2007;37(6):872-9. PubMed PMID: 17517101.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix metalloproteases in vernal keratoconjunctivitis, nasal polyps and allergic asthma. AU - Leonardi,A, AU - Brun,P, AU - Di Stefano,A, AU - Motterle,L, AU - Abatangelo,G, PY - 2007/5/23/pubmed PY - 2007/11/9/medline PY - 2007/5/23/entrez SP - 872 EP - 9 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 37 IS - 6 N2 - BACKGROUND: Allergic conditions in different organs share many similarities in their inflammatory response. Vernal keratoconjunctivitis (VKC), asthma and nasal polyps exhibit several similar, but site-specific mucosal structural changes. The aim of the study was to investigate whether matrix metalloproteases contribute to different tissue remodelling aspects in different organs. METHODS: Mucosal biopsies were obtained from conjunctiva of healthy donors, tarsal conjunctiva of vernal patients, bronchi of non-asthmatic subjects, bronchi of mild stable asthmatic patients, nasal mucosa of non-allergic donors and nasal polyps of allergic patients. Distribution of metalloprotease-1, -3, -9, -13, tissue inhibitor of metalloproteases-1, collagens I and III and the presence of eosinophils and CD4+ cells were evaluated by immunohistochemistry. RESULTS: Collagens were highly diffuse in the giant papillae of VKC and in nasal polyps, and yet less increased in the subepithelium of asthmatic patients. Immunostaining for metalloprotease-1, -3, -9 and -13 was significantly higher in VKC compared with normal conjunctiva. Metalloprotease-9 staining was higher in the stroma of polyps vs. normal nasal mucosa, and only metalloprotease-13 was significantly more expressed in asthmatic vs. non-asthmatic subjects. Metalloprotease-9 immunostaining was more intense in vernal compared with other tissues. In all pathological tissues, metalloprotease-9-positive staining was in association with eosinophils and CD4+ cells. CONCLUSIONS: Expression of metalloproteases may play an important role in inducing the structural changes seen in VKC, nasal polyps and asthma. Tissue remodelling and gelatinase immunoexpression was more dramatic in giant papillae of vernal patients compared with other tissue sites of chronic allergic inflammation. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/17517101/Matrix_metalloproteases_in_vernal_keratoconjunctivitis_nasal_polyps_and_allergic_asthma_ L2 - https://doi.org/10.1111/j.1365-2222.2007.02732.x DB - PRIME DP - Unbound Medicine ER -