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Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury.
Biochem Biophys Res Commun. 2007 Jul 13; 358(4):1058-64.BB

Abstract

Thermosensitive TRP channels display unique thermal responses, suggesting distinct roles mediating sensory transmission of temperature. However, whether relative expression of these channels in dorsal root ganglia (DRG) is altered in nerve injury is unknown. We developed a multiplex ribonuclease protection assay (RPA) to quantify rat TRPV1, TRPV2, TRPV3, TRPV4, TRPA1, and TRPM8 RNA levels in DRG. We used the multiplex RPA to measure thermosensitive TRP channel RNA levels in DRG from RTX-treated rats (300 microg/kg) or rats with unilateral sciatic nerve chronic constriction injury (CCI). TRPV1 and TRPA1 RNA were significantly decreased in DRG from RTX-treated rats, indicating functional colocalization of TRPA1 and TRPV1 in sensory nociceptors. In DRG from CCI rats, TRPA1, TRPV2, and TRPM8 RNA showed slight but significant increases ipsilateral to peripheral nerve injury. Our findings support the hypothesis that increased TRP channel expression in sensory neurons may contribute to mechanical and cold hypersensitivity.

Authors+Show Affiliations

Western Connecticut State University, 181 White Street, Danbury, CT 06810, USA. frederickj@wcsu.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17517374

Citation

Frederick, J, et al. "Increased TRPA1, TRPM8, and TRPV2 Expression in Dorsal Root Ganglia By Nerve Injury." Biochemical and Biophysical Research Communications, vol. 358, no. 4, 2007, pp. 1058-64.
Frederick J, Buck ME, Matson DJ, et al. Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury. Biochem Biophys Res Commun. 2007;358(4):1058-64.
Frederick, J., Buck, M. E., Matson, D. J., & Cortright, D. N. (2007). Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury. Biochemical and Biophysical Research Communications, 358(4), 1058-64.
Frederick J, et al. Increased TRPA1, TRPM8, and TRPV2 Expression in Dorsal Root Ganglia By Nerve Injury. Biochem Biophys Res Commun. 2007 Jul 13;358(4):1058-64. PubMed PMID: 17517374.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury. AU - Frederick,J, AU - Buck,M E, AU - Matson,D J, AU - Cortright,D N, Y1 - 2007/05/14/ PY - 2007/04/25/received PY - 2007/05/05/accepted PY - 2007/5/23/pubmed PY - 2007/8/10/medline PY - 2007/5/23/entrez SP - 1058 EP - 64 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 358 IS - 4 N2 - Thermosensitive TRP channels display unique thermal responses, suggesting distinct roles mediating sensory transmission of temperature. However, whether relative expression of these channels in dorsal root ganglia (DRG) is altered in nerve injury is unknown. We developed a multiplex ribonuclease protection assay (RPA) to quantify rat TRPV1, TRPV2, TRPV3, TRPV4, TRPA1, and TRPM8 RNA levels in DRG. We used the multiplex RPA to measure thermosensitive TRP channel RNA levels in DRG from RTX-treated rats (300 microg/kg) or rats with unilateral sciatic nerve chronic constriction injury (CCI). TRPV1 and TRPA1 RNA were significantly decreased in DRG from RTX-treated rats, indicating functional colocalization of TRPA1 and TRPV1 in sensory nociceptors. In DRG from CCI rats, TRPA1, TRPV2, and TRPM8 RNA showed slight but significant increases ipsilateral to peripheral nerve injury. Our findings support the hypothesis that increased TRP channel expression in sensory neurons may contribute to mechanical and cold hypersensitivity. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/17517374/Increased_TRPA1_TRPM8_and_TRPV2_expression_in_dorsal_root_ganglia_by_nerve_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(07)00985-0 DB - PRIME DP - Unbound Medicine ER -