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Phenotypic and genotypic characterization of Paenibacillus larvae isolates.
Vet Microbiol. 2007 Sep 20; 124(1-2):178-83.VM

Abstract

Paenibacillus larvae is the causative agent of American Foulbrood (AFB), a severe disease of honeybees (Apis melifera). The aim of this work was to develop a strategy for the subtyping and the epidemiological analysis of P. larvae. Phenotypic characterisation, susceptibility to several antibiotics, electrophoresis of whole bacterial proteins, rep-PCR, ribotyping and DGGE were assessed using a collection of P. larvae isolates from different Uruguayan and Argentinean locations. Results indicated that there are two P. larvae genotypes circulating in Uruguay ERIC I-BOX A (worldwide distributed) and ERIC I-BOX C (exclusively detected in Argentina until this study). These results suggest that P. larvae isolates had moved between Argentina and Uruguay, probably through the Uruguay River. Patterns of whole bacterial proteins, DGGE and ribotyping did not improve the P. larvae intraspecific discrimination. Antibiotic susceptibility assays showed that 100% isolates were OTC-sensitive and 22% (belonging to ERIC I-BOX A group) were sulfisoxazole-resistant. This work may contribute to the elucidation of basic aspects related to the epidemiology of AFB in Uruguay and in the region.

Authors+Show Affiliations

Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318, C.P. 11600 Montevideo, Uruguay.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17517481

Citation

Antúnez, K, et al. "Phenotypic and Genotypic Characterization of Paenibacillus Larvae Isolates." Veterinary Microbiology, vol. 124, no. 1-2, 2007, pp. 178-83.
Antúnez K, Piccini C, Castro-Sowinski S, et al. Phenotypic and genotypic characterization of Paenibacillus larvae isolates. Vet Microbiol. 2007;124(1-2):178-83.
Antúnez, K., Piccini, C., Castro-Sowinski, S., Rosado, A. S., Seldin, L., & Zunino, P. (2007). Phenotypic and genotypic characterization of Paenibacillus larvae isolates. Veterinary Microbiology, 124(1-2), 178-83.
Antúnez K, et al. Phenotypic and Genotypic Characterization of Paenibacillus Larvae Isolates. Vet Microbiol. 2007 Sep 20;124(1-2):178-83. PubMed PMID: 17517481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic and genotypic characterization of Paenibacillus larvae isolates. AU - Antúnez,K, AU - Piccini,C, AU - Castro-Sowinski,S, AU - Rosado,A S, AU - Seldin,L, AU - Zunino,P, Y1 - 2007/04/08/ PY - 2007/02/06/received PY - 2007/03/30/revised PY - 2007/04/04/accepted PY - 2007/5/23/pubmed PY - 2007/12/27/medline PY - 2007/5/23/entrez SP - 178 EP - 83 JF - Veterinary microbiology JO - Vet Microbiol VL - 124 IS - 1-2 N2 - Paenibacillus larvae is the causative agent of American Foulbrood (AFB), a severe disease of honeybees (Apis melifera). The aim of this work was to develop a strategy for the subtyping and the epidemiological analysis of P. larvae. Phenotypic characterisation, susceptibility to several antibiotics, electrophoresis of whole bacterial proteins, rep-PCR, ribotyping and DGGE were assessed using a collection of P. larvae isolates from different Uruguayan and Argentinean locations. Results indicated that there are two P. larvae genotypes circulating in Uruguay ERIC I-BOX A (worldwide distributed) and ERIC I-BOX C (exclusively detected in Argentina until this study). These results suggest that P. larvae isolates had moved between Argentina and Uruguay, probably through the Uruguay River. Patterns of whole bacterial proteins, DGGE and ribotyping did not improve the P. larvae intraspecific discrimination. Antibiotic susceptibility assays showed that 100% isolates were OTC-sensitive and 22% (belonging to ERIC I-BOX A group) were sulfisoxazole-resistant. This work may contribute to the elucidation of basic aspects related to the epidemiology of AFB in Uruguay and in the region. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/17517481/Phenotypic_and_genotypic_characterization_of_Paenibacillus_larvae_isolates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(07)00176-9 DB - PRIME DP - Unbound Medicine ER -