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Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae.
Plant J. 2007 Jul; 51(2):234-46.PJ

Abstract

In Arabidopsis, the GH3-like gene family consists of 19 members, several of which have been shown to adenylate the plant hormones jasmonic acid, indole acetic acid and salicylic acid (SA). In some cases, this adenylation has been shown to catalyze hormone conjugation to amino acids. Here we report molecular characterization of the GH3-LIKE DEFENSE GENE 1 (GDG1), a member of the GH3-like gene family, and show that GDG1 is an important component of SA-mediated defense against the bacterial pathogen Pseudomonas syringae. Expression of GDG1 is induced earlier and to a higher level in response to avirulent pathogens compared to virulent pathogens. gdg1 null mutants are compromised in several pathogen defense responses, including activation of defense genes and resistance against virulent and avirulent bacterial pathogens. Accumulation of free and glucoside-conjugated SA (SAG) in response to pathogen infection is compromised in gdg1 mutants. All defense-related phenotypes of gdg1 can be rescued by external application of SA, suggesting that gdg1 mutants are defective in the SA-mediated defense pathway(s) and that GDG1 functions upstream of SA. Our results suggest that GDG1 contributes to both basal and resistance gene-mediated inducible defenses against P. syringae (and possibly other pathogens) by playing a critical role in regulating the levels of pathogen-inducible SA. GDG1 is allelic to the PBS3 (avrPphB susceptible) gene.

Authors+Show Affiliations

Department of Biology, Syracuse University, Syracuse, NY 13244, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17521413

Citation

Jagadeeswaran, Guru, et al. "Arabidopsis GH3-LIKE DEFENSE GENE 1 Is Required for Accumulation of Salicylic Acid, Activation of Defense Responses and Resistance to Pseudomonas Syringae." The Plant Journal : for Cell and Molecular Biology, vol. 51, no. 2, 2007, pp. 234-46.
Jagadeeswaran G, Raina S, Acharya BR, et al. Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae. Plant J. 2007;51(2):234-46.
Jagadeeswaran, G., Raina, S., Acharya, B. R., Maqbool, S. B., Mosher, S. L., Appel, H. M., Schultz, J. C., Klessig, D. F., & Raina, R. (2007). Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae. The Plant Journal : for Cell and Molecular Biology, 51(2), 234-46.
Jagadeeswaran G, et al. Arabidopsis GH3-LIKE DEFENSE GENE 1 Is Required for Accumulation of Salicylic Acid, Activation of Defense Responses and Resistance to Pseudomonas Syringae. Plant J. 2007;51(2):234-46. PubMed PMID: 17521413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Arabidopsis GH3-LIKE DEFENSE GENE 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae. AU - Jagadeeswaran,Guru, AU - Raina,Surabhi, AU - Acharya,Biswa R, AU - Maqbool,Shahina B, AU - Mosher,Stephen L, AU - Appel,Heidi M, AU - Schultz,Jack C, AU - Klessig,Daniel F, AU - Raina,Ramesh, Y1 - 2007/05/23/ PY - 2007/5/25/pubmed PY - 2007/10/10/medline PY - 2007/5/25/entrez SP - 234 EP - 46 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 51 IS - 2 N2 - In Arabidopsis, the GH3-like gene family consists of 19 members, several of which have been shown to adenylate the plant hormones jasmonic acid, indole acetic acid and salicylic acid (SA). In some cases, this adenylation has been shown to catalyze hormone conjugation to amino acids. Here we report molecular characterization of the GH3-LIKE DEFENSE GENE 1 (GDG1), a member of the GH3-like gene family, and show that GDG1 is an important component of SA-mediated defense against the bacterial pathogen Pseudomonas syringae. Expression of GDG1 is induced earlier and to a higher level in response to avirulent pathogens compared to virulent pathogens. gdg1 null mutants are compromised in several pathogen defense responses, including activation of defense genes and resistance against virulent and avirulent bacterial pathogens. Accumulation of free and glucoside-conjugated SA (SAG) in response to pathogen infection is compromised in gdg1 mutants. All defense-related phenotypes of gdg1 can be rescued by external application of SA, suggesting that gdg1 mutants are defective in the SA-mediated defense pathway(s) and that GDG1 functions upstream of SA. Our results suggest that GDG1 contributes to both basal and resistance gene-mediated inducible defenses against P. syringae (and possibly other pathogens) by playing a critical role in regulating the levels of pathogen-inducible SA. GDG1 is allelic to the PBS3 (avrPphB susceptible) gene. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/17521413/Arabidopsis_GH3_LIKE_DEFENSE_GENE_1_is_required_for_accumulation_of_salicylic_acid_activation_of_defense_responses_and_resistance_to_Pseudomonas_syringae_ L2 - https://doi.org/10.1111/j.1365-313X.2007.03130.x DB - PRIME DP - Unbound Medicine ER -