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Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis.
Peptides 2007; 28(6):1304-10P

Abstract

Two cDNAs encoding defensin-like antimicrobial peptides were cloned and sequenced from the tick Haemaphysalis longicornis. The full-length cDNA of Hlgut-defensin (H. longicornis midgut defensin) is 333bp, encoding an expected protein with 73 amino acids. The full-length cDNA of Hlsal-defensin (H. longicornis salivary gland defensin) is 382bp, encoding an expected protein with 81 amino acids. The antibacterial activities of the synthetic peptides based on the Hlgut-defensin and Hlsal-defensin sequences were tested against a variety of Gram-positive and Gram-negative bacteria. Using real-time PCR, the tissue-specific expression of two defensin-like peptides were determined and it was also found that the gene transcripts of Hlgut-defensin and Hlsal-defensin were significantly induced by a lipopolysaccharide (LPS) injection.

Authors+Show Affiliations

Department of Frontier Veterinary Medicine, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17521774

Citation

Zhou, Jinlin, et al. "Sequence Characterization and Expression Patterns of Two Defensin-like Antimicrobial Peptides From the Tick Haemaphysalis Longicornis." Peptides, vol. 28, no. 6, 2007, pp. 1304-10.
Zhou J, Liao M, Ueda M, et al. Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Peptides. 2007;28(6):1304-10.
Zhou, J., Liao, M., Ueda, M., Gong, H., Xuan, X., & Fujisaki, K. (2007). Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. Peptides, 28(6), pp. 1304-10.
Zhou J, et al. Sequence Characterization and Expression Patterns of Two Defensin-like Antimicrobial Peptides From the Tick Haemaphysalis Longicornis. Peptides. 2007;28(6):1304-10. PubMed PMID: 17521774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis. AU - Zhou,Jinlin, AU - Liao,Min, AU - Ueda,Mami, AU - Gong,Haiyan, AU - Xuan,Xuenan, AU - Fujisaki,Kozo, Y1 - 2007/05/06/ PY - 2007/03/05/received PY - 2007/04/30/revised PY - 2007/04/30/accepted PY - 2007/5/25/pubmed PY - 2007/9/6/medline PY - 2007/5/25/entrez SP - 1304 EP - 10 JF - Peptides JO - Peptides VL - 28 IS - 6 N2 - Two cDNAs encoding defensin-like antimicrobial peptides were cloned and sequenced from the tick Haemaphysalis longicornis. The full-length cDNA of Hlgut-defensin (H. longicornis midgut defensin) is 333bp, encoding an expected protein with 73 amino acids. The full-length cDNA of Hlsal-defensin (H. longicornis salivary gland defensin) is 382bp, encoding an expected protein with 81 amino acids. The antibacterial activities of the synthetic peptides based on the Hlgut-defensin and Hlsal-defensin sequences were tested against a variety of Gram-positive and Gram-negative bacteria. Using real-time PCR, the tissue-specific expression of two defensin-like peptides were determined and it was also found that the gene transcripts of Hlgut-defensin and Hlsal-defensin were significantly induced by a lipopolysaccharide (LPS) injection. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/17521774/Sequence_characterization_and_expression_patterns_of_two_defensin_like_antimicrobial_peptides_from_the_tick_Haemaphysalis_longicornis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(07)00160-X DB - PRIME DP - Unbound Medicine ER -