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Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections.
J Clin Virol. 2007 Jul; 39(3):194-8.JC

Abstract

BACKGROUND

Serological assays are widely used to confirm dengue virus infections and to differentiate between a primary and a secondary infection.

OBJECTIVE

Two commercial dengue diagnostic kits, Panbio Dengue IgM Capture and Dengue IgG Capture ELISA (Brisbane, Australia) were evaluated.

STUDY DESIGN

Three hundred and seventy-three serum samples were tested. Panel sera included samples from dengue confirmed cases (representing both primary and secondary infections), from non-dengue infectious diseases, and from healthy individuals. The MAC-ELISA/Dengue IPK was used for the detection of anti-dengue virus IgM antibody in the sera and the ELISA inhibition method (EIM/Dengue IPK) was used to differentiate between primary and secondary infections. Both these reference assays, which were previously developed in the Arbovirus Laboratory at the "Pedro Kouri" Tropical Medicine Institute, were employed as the gold standard.

RESULTS

High sensitivity (96.8%) and specificity (99.4%) were found with the commercial diagnostics when compared to the reference methods. Furthermore, high concordance 95.5% in classifying dengue infection types (primary or secondary infections) was observed.

CONCLUSIONS

The Panbio Dengue IgM and IgG assays offer a good alternative for dengue diagnosis. They are easy to perform and results can be obtained in less than 3h.

Authors+Show Affiliations

"Pedro Kourí" Tropical Medicine Institute, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17521960

Citation

Vazquez, Susana, et al. "Evaluation of Immunoglobulin M and G Capture Enzyme-linked Immunosorbent Assay Panbio Kits for Diagnostic Dengue Infections." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 39, no. 3, 2007, pp. 194-8.
Vazquez S, Hafner G, Ruiz D, et al. Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. J Clin Virol. 2007;39(3):194-8.
Vazquez, S., Hafner, G., Ruiz, D., Calzada, N., & Guzman, M. G. (2007). Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 39(3), 194-8.
Vazquez S, et al. Evaluation of Immunoglobulin M and G Capture Enzyme-linked Immunosorbent Assay Panbio Kits for Diagnostic Dengue Infections. J Clin Virol. 2007;39(3):194-8. PubMed PMID: 17521960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. AU - Vazquez,Susana, AU - Hafner,Greg, AU - Ruiz,Didye, AU - Calzada,Naifi, AU - Guzman,Maria G, Y1 - 2007/05/22/ PY - 2006/04/26/received PY - 2007/03/15/revised PY - 2007/04/03/accepted PY - 2007/5/25/pubmed PY - 2007/8/22/medline PY - 2007/5/25/entrez SP - 194 EP - 8 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 39 IS - 3 N2 - BACKGROUND: Serological assays are widely used to confirm dengue virus infections and to differentiate between a primary and a secondary infection. OBJECTIVE: Two commercial dengue diagnostic kits, Panbio Dengue IgM Capture and Dengue IgG Capture ELISA (Brisbane, Australia) were evaluated. STUDY DESIGN: Three hundred and seventy-three serum samples were tested. Panel sera included samples from dengue confirmed cases (representing both primary and secondary infections), from non-dengue infectious diseases, and from healthy individuals. The MAC-ELISA/Dengue IPK was used for the detection of anti-dengue virus IgM antibody in the sera and the ELISA inhibition method (EIM/Dengue IPK) was used to differentiate between primary and secondary infections. Both these reference assays, which were previously developed in the Arbovirus Laboratory at the "Pedro Kouri" Tropical Medicine Institute, were employed as the gold standard. RESULTS: High sensitivity (96.8%) and specificity (99.4%) were found with the commercial diagnostics when compared to the reference methods. Furthermore, high concordance 95.5% in classifying dengue infection types (primary or secondary infections) was observed. CONCLUSIONS: The Panbio Dengue IgM and IgG assays offer a good alternative for dengue diagnosis. They are easy to perform and results can be obtained in less than 3h. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/17521960/Evaluation_of_immunoglobulin_M_and_G_capture_enzyme_linked_immunosorbent_assay_Panbio_kits_for_diagnostic_dengue_infections_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(07)00131-X DB - PRIME DP - Unbound Medicine ER -