Tags

Type your tag names separated by a space and hit enter

Association between decreased vitamin levels and MTHFR, MTR and MTRR gene polymorphisms as determinants for elevated total homocysteine concentrations in pregnant women.
Eur J Clin Nutr 2008; 62(8):1010-21EJ

Abstract

OBJECTIVES

To examine the association between methylenetetrahydrofolate reductase (MTHFR) (C677T and A1298C), methionine synthase (MTR) A2756G and methionine synthase reductase (MTRR) A66G gene polymorphisms and total homocysteine (tHcy), methylmalonic acid (MMA) and S-adenosylmethionine/S-adenosylhomocysteine (SAM/SAH) levels; and to evaluate the potential interactions with folate or cobalamin (Cbl) status.

SUBJECTS/METHODS

Two hundred seventy-five healthy women at labor who delivered full-term normal babies. Cbl, folate, tHcy, MMA, SAM and SAH were measured in serum specimens. The genotypes for polymorphisms were determined by PCR-restriction fragment length polymorphism (RFLP).

RESULTS

Serum folate, MTHFR 677T allele and MTR 2756AA genotypes were the predictors of tHcy levels in pregnant women. Serum Cbl and creatinine were the predictors of SAM/SAH ratio and MMA levels, respectively. The gene polymorphisms were not determinants for MMA levels and SAM/SAH ratios. Low levels of serum folate were associated with elevated tHcy in pregnant women, independently of the gene polymorphisms. In pregnant women carrying MTHFR 677T allele, or MTHFR 1298AA or MTRR 66AA genotypes, lower Cbl levels were associated with higher levels of tHcy. Lower SAM/SAH ratio was found in MTHFR 677CC or MTRR A2756AA genotypes carriers when Cbl levels were lower than 142 pmol/l.

CONCLUSIONS

Serum folate and MTHFR C677T and MTR A2576G gene polymorphisms were the determinants for tHcy levels. The interaction between low levels of serum Cbl and MTHFR (C677T or A1298C) or MTRR A66G gene polymorphisms was associated with increased tHcy.

Authors+Show Affiliations

Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, São Paulo, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17522601

Citation

Barbosa, P R., et al. "Association Between Decreased Vitamin Levels and MTHFR, MTR and MTRR Gene Polymorphisms as Determinants for Elevated Total Homocysteine Concentrations in Pregnant Women." European Journal of Clinical Nutrition, vol. 62, no. 8, 2008, pp. 1010-21.
Barbosa PR, Stabler SP, Machado AL, et al. Association between decreased vitamin levels and MTHFR, MTR and MTRR gene polymorphisms as determinants for elevated total homocysteine concentrations in pregnant women. Eur J Clin Nutr. 2008;62(8):1010-21.
Barbosa, P. R., Stabler, S. P., Machado, A. L., Braga, R. C., Hirata, R. D., Hirata, M. H., ... Guerra-Shinohara, E. M. (2008). Association between decreased vitamin levels and MTHFR, MTR and MTRR gene polymorphisms as determinants for elevated total homocysteine concentrations in pregnant women. European Journal of Clinical Nutrition, 62(8), pp. 1010-21.
Barbosa PR, et al. Association Between Decreased Vitamin Levels and MTHFR, MTR and MTRR Gene Polymorphisms as Determinants for Elevated Total Homocysteine Concentrations in Pregnant Women. Eur J Clin Nutr. 2008;62(8):1010-21. PubMed PMID: 17522601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between decreased vitamin levels and MTHFR, MTR and MTRR gene polymorphisms as determinants for elevated total homocysteine concentrations in pregnant women. AU - Barbosa,P R, AU - Stabler,S P, AU - Machado,A L K, AU - Braga,R C, AU - Hirata,R D C, AU - Hirata,M H, AU - Sampaio-Neto,L F, AU - Allen,R H, AU - Guerra-Shinohara,E M, Y1 - 2007/05/23/ PY - 2007/5/25/pubmed PY - 2009/1/1/medline PY - 2007/5/25/entrez SP - 1010 EP - 21 JF - European journal of clinical nutrition JO - Eur J Clin Nutr VL - 62 IS - 8 N2 - OBJECTIVES: To examine the association between methylenetetrahydrofolate reductase (MTHFR) (C677T and A1298C), methionine synthase (MTR) A2756G and methionine synthase reductase (MTRR) A66G gene polymorphisms and total homocysteine (tHcy), methylmalonic acid (MMA) and S-adenosylmethionine/S-adenosylhomocysteine (SAM/SAH) levels; and to evaluate the potential interactions with folate or cobalamin (Cbl) status. SUBJECTS/METHODS: Two hundred seventy-five healthy women at labor who delivered full-term normal babies. Cbl, folate, tHcy, MMA, SAM and SAH were measured in serum specimens. The genotypes for polymorphisms were determined by PCR-restriction fragment length polymorphism (RFLP). RESULTS: Serum folate, MTHFR 677T allele and MTR 2756AA genotypes were the predictors of tHcy levels in pregnant women. Serum Cbl and creatinine were the predictors of SAM/SAH ratio and MMA levels, respectively. The gene polymorphisms were not determinants for MMA levels and SAM/SAH ratios. Low levels of serum folate were associated with elevated tHcy in pregnant women, independently of the gene polymorphisms. In pregnant women carrying MTHFR 677T allele, or MTHFR 1298AA or MTRR 66AA genotypes, lower Cbl levels were associated with higher levels of tHcy. Lower SAM/SAH ratio was found in MTHFR 677CC or MTRR A2756AA genotypes carriers when Cbl levels were lower than 142 pmol/l. CONCLUSIONS: Serum folate and MTHFR C677T and MTR A2576G gene polymorphisms were the determinants for tHcy levels. The interaction between low levels of serum Cbl and MTHFR (C677T or A1298C) or MTRR A66G gene polymorphisms was associated with increased tHcy. SN - 0954-3007 UR - https://www.unboundmedicine.com/medline/citation/17522601/Association_between_decreased_vitamin_levels_and_MTHFR_MTR_and_MTRR_gene_polymorphisms_as_determinants_for_elevated_total_homocysteine_concentrations_in_pregnant_women_ L2 - http://dx.doi.org/10.1038/sj.ejcn.1602810 DB - PRIME DP - Unbound Medicine ER -