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The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid.
Photodermatol Photoimmunol Photomed. 2007 Apr-Jun; 23(2-3):95-7.PP

Abstract

BACKGROUND

Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp.

METHODS

The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay.

RESULTS

In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA.

CONCLUSION

Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect.

Authors+Show Affiliations

Department of Dermatology, Aichi Medical University School of Medicine, Nagakute, Japan. 106039@gk.amu.aichi-med-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

17523931

Citation

Akita, Yoichi, et al. "The Effect of Psoralen Plus Ultraviolet a in Vitro in HUT-78 Enhances By 5-aminolevulinic Acid." Photodermatology, Photoimmunology & Photomedicine, vol. 23, no. 2-3, 2007, pp. 95-7.
Akita Y, Watanabe D, Yanagishita T, et al. The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid. Photodermatol Photoimmunol Photomed. 2007;23(2-3):95-7.
Akita, Y., Watanabe, D., Yanagishita, T., Kuhara, T., Kawamura, C., Masuda, Y., Kawada, M., Nakaseko, H., Tamada, Y., & Matsumoto, Y. (2007). The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid. Photodermatology, Photoimmunology & Photomedicine, 23(2-3), 95-7.
Akita Y, et al. The Effect of Psoralen Plus Ultraviolet a in Vitro in HUT-78 Enhances By 5-aminolevulinic Acid. Photodermatol Photoimmunol Photomed. 2007 Apr-Jun;23(2-3):95-7. PubMed PMID: 17523931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid. AU - Akita,Yoichi, AU - Watanabe,Daisuke, AU - Yanagishita,Takeshi, AU - Kuhara,Tomoe, AU - Kawamura,Chiharu, AU - Masuda,Yuko, AU - Kawada,Morihiro, AU - Nakaseko,Hirono, AU - Tamada,Yasuhiko, AU - Matsumoto,Yoshinari, PY - 2007/5/26/pubmed PY - 2007/6/21/medline PY - 2007/5/26/entrez SP - 95 EP - 7 JF - Photodermatology, photoimmunology & photomedicine JO - Photodermatol Photoimmunol Photomed VL - 23 IS - 2-3 N2 - BACKGROUND: Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp. METHODS: The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay. RESULTS: In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA. CONCLUSION: Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect. SN - 0905-4383 UR - https://www.unboundmedicine.com/medline/citation/17523931/The_effect_of_psoralen_plus_ultraviolet_A_in_vitro_in_HUT_78_enhances_by_5_aminolevulinic_acid_ L2 - https://doi.org/10.1111/j.1600-0781.2007.00280.x DB - PRIME DP - Unbound Medicine ER -