TY - JOUR T1 - The effect of psoralen plus ultraviolet A in vitro in HUT-78 enhances by 5-aminolevulinic acid. AU - Akita,Yoichi, AU - Watanabe,Daisuke, AU - Yanagishita,Takeshi, AU - Kuhara,Tomoe, AU - Kawamura,Chiharu, AU - Masuda,Yuko, AU - Kawada,Morihiro, AU - Nakaseko,Hirono, AU - Tamada,Yasuhiko, AU - Matsumoto,Yoshinari, PY - 2007/5/26/pubmed PY - 2007/6/21/medline PY - 2007/5/26/entrez SP - 95 EP - 7 JF - Photodermatology, photoimmunology & photomedicine JO - Photodermatol Photoimmunol Photomed VL - 23 IS - 2-3 N2 - BACKGROUND: Sezary syndrome and mycosis fungoides are forms of cutaneous T-cell lymphoma, and in the early stage of these diseases psoralen plus ultraviolet A (PUVA) is one of the treatments of choice. Photodynamic therapy using 5-aminolevulinic acid (ALA-PDT) is an effective, non-invasive, and safe treatment for most superficial skin cancers. In order to obtain greater efficacy of PUVA, we investigated the synergistic anti-tumor effects of ALA-PDT and PUVA using 8-methoxypsoralen (8-MOP) and a UVA lamp. METHODS: The in vitro effects of PUVA and ALA-PDT and their combination in HUT-78 cell line from human SS were determined by MTT assay. RESULTS: In our results, cell proliferation compared with controls was inhibited to 53.2% with UVA alone, 52.3% with 1 microM 8-MOP, 43.8% with 100 microM ALA, and 19.2% with combined 8-MOP and ALA. CONCLUSION: Combined use of ALA and PUVA using 8-MOP and UVA lamps, which are widespread in Japan, had a strong anti-tumor effect in vitro. Combined treatment with ALA-PDT and PUVA using a UVA lamp appears to have a strong treatment effect. SN - 0905-4383 UR - https://www.unboundmedicine.com/medline/citation/17523931/The_effect_of_psoralen_plus_ultraviolet_A_in_vitro_in_HUT_78_enhances_by_5_aminolevulinic_acid_ L2 - https://doi.org/10.1111/j.1600-0781.2007.00280.x DB - PRIME DP - Unbound Medicine ER -