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Anti-oxidative/oxidative status of rat liver after ovariectomy.
J Vet Med A Physiol Pathol Clin Med. 2007 Jun; 54(5):225-9.JV

Abstract

The lack of protective action of oestrogens which appears during menopausal period may be the reason of serious metabolic disturbances including oxidative stress. The hypothesis was stated that ovariectomy may induce the variations of antioxidant/oxidant status which can be detected in rat liver. A total of 102 healthy Wistar female rats were included in the experiment and divided into control (CON; n = 6), sham-operated (SHO; n = 48) and ovariectomized (OVX; n = 48) groups. Animals from SHO (n = 6) and OVX (n = 6) groups were killed every week during 8 weeks of experiment to detect dynamic changes in examined parameters. Anti-oxidative enzyme activities [glutathione peroxidase (GSH-Px); superoxide dismutase (SOD)] as well as total antioxidant capacity (TAC) and the intensity of lipid peroxidation, measured by the concentration of N,N,diethyl-p-phenylene diamine (DEPPD) radical, were determined in liver homogenates by the use of spectrophotometric methods. Wave-like patterns of examined parameters within 8 weeks of experiment were detected. GSH-Px activity tended to be higher in OVX animals and was significantly lower at 8th week when compared with 1st week of experiment. SOD activity was higher in SHO animals and showed significant differences between 3rd, 4th, 7th and 1st week. TAC values were significantly higher in OVX when compared with SHO groups in 2nd, 4th, 5th week and significantly lower in 3rd, 6th and 7th week of the experiment. The concentration of DEPPD radicals tended to increase in OVX group. In conclusion, ovariectomy which leads to oestrogen insufficiency is reflected as well in variation of anti-oxidative/oxidative parameters in rat liver homogenates.

Authors+Show Affiliations

Department of Animal Biochemistry and Physiology, Faculty of Veterinary Medicine, Agricultural University, 20-033 Lublin, Akademicka 13, Poland. marta.kankofer@ar.lublin.plNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17523954

Citation

Kankofer, M, et al. "Anti-oxidative/oxidative Status of Rat Liver After Ovariectomy." Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, vol. 54, no. 5, 2007, pp. 225-9.
Kankofer M, Radzki RP, Bieńko M, et al. Anti-oxidative/oxidative status of rat liver after ovariectomy. J Vet Med A Physiol Pathol Clin Med. 2007;54(5):225-9.
Kankofer, M., Radzki, R. P., Bieńko, M., & Albera, E. (2007). Anti-oxidative/oxidative status of rat liver after ovariectomy. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, 54(5), 225-9.
Kankofer M, et al. Anti-oxidative/oxidative Status of Rat Liver After Ovariectomy. J Vet Med A Physiol Pathol Clin Med. 2007;54(5):225-9. PubMed PMID: 17523954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-oxidative/oxidative status of rat liver after ovariectomy. AU - Kankofer,M, AU - Radzki,R P, AU - Bieńko,M, AU - Albera,E, PY - 2007/5/26/pubmed PY - 2007/8/7/medline PY - 2007/5/26/entrez SP - 225 EP - 9 JF - Journal of veterinary medicine. A, Physiology, pathology, clinical medicine JO - J Vet Med A Physiol Pathol Clin Med VL - 54 IS - 5 N2 - The lack of protective action of oestrogens which appears during menopausal period may be the reason of serious metabolic disturbances including oxidative stress. The hypothesis was stated that ovariectomy may induce the variations of antioxidant/oxidant status which can be detected in rat liver. A total of 102 healthy Wistar female rats were included in the experiment and divided into control (CON; n = 6), sham-operated (SHO; n = 48) and ovariectomized (OVX; n = 48) groups. Animals from SHO (n = 6) and OVX (n = 6) groups were killed every week during 8 weeks of experiment to detect dynamic changes in examined parameters. Anti-oxidative enzyme activities [glutathione peroxidase (GSH-Px); superoxide dismutase (SOD)] as well as total antioxidant capacity (TAC) and the intensity of lipid peroxidation, measured by the concentration of N,N,diethyl-p-phenylene diamine (DEPPD) radical, were determined in liver homogenates by the use of spectrophotometric methods. Wave-like patterns of examined parameters within 8 weeks of experiment were detected. GSH-Px activity tended to be higher in OVX animals and was significantly lower at 8th week when compared with 1st week of experiment. SOD activity was higher in SHO animals and showed significant differences between 3rd, 4th, 7th and 1st week. TAC values were significantly higher in OVX when compared with SHO groups in 2nd, 4th, 5th week and significantly lower in 3rd, 6th and 7th week of the experiment. The concentration of DEPPD radicals tended to increase in OVX group. In conclusion, ovariectomy which leads to oestrogen insufficiency is reflected as well in variation of anti-oxidative/oxidative parameters in rat liver homogenates. SN - 0931-184X UR - https://www.unboundmedicine.com/medline/citation/17523954/Anti_oxidative/oxidative_status_of_rat_liver_after_ovariectomy_ L2 - https://doi.org/10.1111/j.1439-0442.2007.00916.x DB - PRIME DP - Unbound Medicine ER -