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Determination of Bitter Orange alkaloids in dietary supplements standard reference materials by liquid chromatography with ultraviolet absorbance and fluorescence detection.
J Chromatogr A. 2007 Jul 13; 1156(1-2):304-11.JC

Abstract

Four adrenergic amines [synephrine, octopamine, tyramine, and n-methyltyramine] were determined in a variety of Bitter Orange containing dietary supplements. Two extraction techniques were evaluated in detail: Soxhlet extraction and sonication extraction. A liquid chromatographic separation using a reversed-phase C(18) stationary phase and the ion-pairing reagent sodium dodecyl sulfate was developed to separate the Bitter Orange alkaloids. Ultraviolet absorbance detection at 220 nm and fluorescence detection with excitation at 273 nm and emission at 304 nm were used for the alkaloid detection. The method described was used for the assignment of the levels of the predominant alkaloids in three candidate standard reference materials containing Bitter Orange.

Authors+Show Affiliations

Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA. putzbach.karsten@rcc.chNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17524413

Citation

Putzbach, Karsten, et al. "Determination of Bitter Orange Alkaloids in Dietary Supplements Standard Reference Materials By Liquid Chromatography With Ultraviolet Absorbance and Fluorescence Detection." Journal of Chromatography. A, vol. 1156, no. 1-2, 2007, pp. 304-11.
Putzbach K, Rimmer CA, Sharpless KE, et al. Determination of Bitter Orange alkaloids in dietary supplements standard reference materials by liquid chromatography with ultraviolet absorbance and fluorescence detection. J Chromatogr A. 2007;1156(1-2):304-11.
Putzbach, K., Rimmer, C. A., Sharpless, K. E., & Sander, L. C. (2007). Determination of Bitter Orange alkaloids in dietary supplements standard reference materials by liquid chromatography with ultraviolet absorbance and fluorescence detection. Journal of Chromatography. A, 1156(1-2), 304-11.
Putzbach K, et al. Determination of Bitter Orange Alkaloids in Dietary Supplements Standard Reference Materials By Liquid Chromatography With Ultraviolet Absorbance and Fluorescence Detection. J Chromatogr A. 2007 Jul 13;1156(1-2):304-11. PubMed PMID: 17524413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Bitter Orange alkaloids in dietary supplements standard reference materials by liquid chromatography with ultraviolet absorbance and fluorescence detection. AU - Putzbach,Karsten, AU - Rimmer,Catherine A, AU - Sharpless,Katherine E, AU - Sander,Lane C, Y1 - 2007/05/23/ PY - 2006/07/27/received PY - 2006/12/01/revised PY - 2006/12/06/accepted PY - 2007/5/26/pubmed PY - 2007/9/26/medline PY - 2007/5/26/entrez SP - 304 EP - 11 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1156 IS - 1-2 N2 - Four adrenergic amines [synephrine, octopamine, tyramine, and n-methyltyramine] were determined in a variety of Bitter Orange containing dietary supplements. Two extraction techniques were evaluated in detail: Soxhlet extraction and sonication extraction. A liquid chromatographic separation using a reversed-phase C(18) stationary phase and the ion-pairing reagent sodium dodecyl sulfate was developed to separate the Bitter Orange alkaloids. Ultraviolet absorbance detection at 220 nm and fluorescence detection with excitation at 273 nm and emission at 304 nm were used for the alkaloid detection. The method described was used for the assignment of the levels of the predominant alkaloids in three candidate standard reference materials containing Bitter Orange. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17524413/Determination_of_Bitter_Orange_alkaloids_in_dietary_supplements_standard_reference_materials_by_liquid_chromatography_with_ultraviolet_absorbance_and_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)02346-6 DB - PRIME DP - Unbound Medicine ER -