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Vaccination of cattle with Danish and Pasteur strains of Mycobacterium bovis BCG induce different levels of IFNgamma post-vaccination, but induce similar levels of protection against bovine tuberculosis.
Vet Immunol Immunopathol 2007; 118(1-2):50-8VI

Abstract

A number of studies have demonstrated significant protection of cattle against bovine tuberculosis following vaccination with the Pasteur strain of Mycobacterium bovis bacille Calmete-Guerin (BCG). However, it is unclear if other daughter strains of BCG are as effective, which is an important issue to resolve for a variety of regulatory compliance issues. This study compared the protective immune responses to bovine tuberculosis induced in cattle vaccinated with BCG Danish with those induced by BCG Pasteur. Groups of calves (n=10) were vaccinated with 10(6) colony forming units (CFU) BCG Pasteur prepared from a fresh liquid culture, 10(6) CFU BCG Danish prepared from a fresh liquid culture or 0.4 mg of reconstituted freeze-dried culture of BCG Danish. Another group (n=10) served as non-vaccinated controls. BCG Pasteur induced significantly higher and more sustained levels of bovine purified protein derivative (PPD)-specific gamma interferon (IFN-gamma) in whole-blood cultures following vaccination compared to either fresh culture BCG Danish or freeze-dried BCG Danish. Vaccination with a fresh culture of BCG Pasteur, fresh culture BCG Danish and freeze-dried BCG Danish gave a significant enhancement in three, four and three pathological and microbiological parameters of protection, respectively, compared to the non-vaccinated group. These results demonstrate the Danish strain of BCG is a viable alternative to BCG Pasteur for vaccination of cattle as both strains had similar efficacy and there was little difference between freshly cultured and freeze-dried formulation of BCG Danish. The results also show that post-vaccination antigen-specific IFN-gamma levels in whole blood is not always a reliable indicator of protection against a subsequent virulent challenge.

Authors+Show Affiliations

AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. neil.wedlock@agresearch.co.nzNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17524495

Citation

Wedlock, D Neil, et al. "Vaccination of Cattle With Danish and Pasteur Strains of Mycobacterium Bovis BCG Induce Different Levels of IFNgamma Post-vaccination, but Induce Similar Levels of Protection Against Bovine Tuberculosis." Veterinary Immunology and Immunopathology, vol. 118, no. 1-2, 2007, pp. 50-8.
Wedlock DN, Denis M, Vordermeier HM, et al. Vaccination of cattle with Danish and Pasteur strains of Mycobacterium bovis BCG induce different levels of IFNgamma post-vaccination, but induce similar levels of protection against bovine tuberculosis. Vet Immunol Immunopathol. 2007;118(1-2):50-8.
Wedlock, D. N., Denis, M., Vordermeier, H. M., Hewinson, R. G., & Buddle, B. M. (2007). Vaccination of cattle with Danish and Pasteur strains of Mycobacterium bovis BCG induce different levels of IFNgamma post-vaccination, but induce similar levels of protection against bovine tuberculosis. Veterinary Immunology and Immunopathology, 118(1-2), pp. 50-8.
Wedlock DN, et al. Vaccination of Cattle With Danish and Pasteur Strains of Mycobacterium Bovis BCG Induce Different Levels of IFNgamma Post-vaccination, but Induce Similar Levels of Protection Against Bovine Tuberculosis. Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):50-8. PubMed PMID: 17524495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vaccination of cattle with Danish and Pasteur strains of Mycobacterium bovis BCG induce different levels of IFNgamma post-vaccination, but induce similar levels of protection against bovine tuberculosis. AU - Wedlock,D Neil, AU - Denis,Michel, AU - Vordermeier,H Martin, AU - Hewinson,R Glyn, AU - Buddle,Bryce M, Y1 - 2007/04/19/ PY - 2006/11/24/received PY - 2007/03/14/revised PY - 2007/04/12/accepted PY - 2007/5/26/pubmed PY - 2007/9/22/medline PY - 2007/5/26/entrez SP - 50 EP - 8 JF - Veterinary immunology and immunopathology JO - Vet. Immunol. Immunopathol. VL - 118 IS - 1-2 N2 - A number of studies have demonstrated significant protection of cattle against bovine tuberculosis following vaccination with the Pasteur strain of Mycobacterium bovis bacille Calmete-Guerin (BCG). However, it is unclear if other daughter strains of BCG are as effective, which is an important issue to resolve for a variety of regulatory compliance issues. This study compared the protective immune responses to bovine tuberculosis induced in cattle vaccinated with BCG Danish with those induced by BCG Pasteur. Groups of calves (n=10) were vaccinated with 10(6) colony forming units (CFU) BCG Pasteur prepared from a fresh liquid culture, 10(6) CFU BCG Danish prepared from a fresh liquid culture or 0.4 mg of reconstituted freeze-dried culture of BCG Danish. Another group (n=10) served as non-vaccinated controls. BCG Pasteur induced significantly higher and more sustained levels of bovine purified protein derivative (PPD)-specific gamma interferon (IFN-gamma) in whole-blood cultures following vaccination compared to either fresh culture BCG Danish or freeze-dried BCG Danish. Vaccination with a fresh culture of BCG Pasteur, fresh culture BCG Danish and freeze-dried BCG Danish gave a significant enhancement in three, four and three pathological and microbiological parameters of protection, respectively, compared to the non-vaccinated group. These results demonstrate the Danish strain of BCG is a viable alternative to BCG Pasteur for vaccination of cattle as both strains had similar efficacy and there was little difference between freshly cultured and freeze-dried formulation of BCG Danish. The results also show that post-vaccination antigen-specific IFN-gamma levels in whole blood is not always a reliable indicator of protection against a subsequent virulent challenge. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/17524495/Vaccination_of_cattle_with_Danish_and_Pasteur_strains_of_Mycobacterium_bovis_BCG_induce_different_levels_of_IFNgamma_post_vaccination_but_induce_similar_levels_of_protection_against_bovine_tuberculosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(07)00134-1 DB - PRIME DP - Unbound Medicine ER -