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Possible involvement of supraspinal opioid and GABA receptors in CDP-choline-induced antinociception in acute pain models in rats.
Neurosci Lett. 2007 Jun 13; 420(2):116-21.NL

Abstract

Cytidine-5'-diphosphate choline (CDP-choline; citicoline) is an essential endogenous compound normally produced by the organism and is a source of cytidine and choline. Our recent studies on acute pain models demonstrate that intracerebroventricularly administered CDP-choline produces antinociception via supraspinal alpha-7 nicotinic acetylcholine receptors-mediated mechanism in rats. However, it remains to be elucidated which other supraspinal mechanisms are involved in the antinociceptive effect of CDP-choline. In this study, we investigated the role of the supraspinal opioidergic, GABAergic, alpha-adrenergic and serotonergic receptors in CDP-choline-induced antinociception. The antinociceptive effect of CDP-choline was evoked by the intracerebroventricular (i.c.v.) administration. Two different pain models were utilized: thermal paw withdrawal test and mechanical paw pressure test. The i.c.v. administration of CDP-choline (0.5, 1.0 and 2.0 micromol) produced dose-dependent antinociception. Non-specific opioid receptor antagonist naloxone (10 microg; i.c.v.) and GABA(B) receptor antagonist CGP-35348 (20 microg; i.c.v.) pretreatments inhibited the antinociceptive effects of CDP-choline (1.0 micromol; i.c.v.). In contrast, the alpha-1 adrenergic receptor antagonist prazosin (20 microg; i.c.v.), alpha-2 adrenergic receptor antagonist yohimbine (30 microg; i.c.v.) and non-specific serotonin receptor antagonist methysergide (20 microg; i.c.v.) pretreatments had no effect on CDP-choline-induced antinociception in the thermal paw withdrawal test and in the mechanical paw pressure test. Therefore, it can be postulated that i.c.v. administered CDP-choline exerts antinociceptive effect mediated by supraspinal opioid and GABA(B) receptors in acute pain models. Furthermore, supraspinal alpha-adrenergic and serotonergic receptors do not appear to be involved in the antinociceptive effect of CDP-choline.

Authors+Show Affiliations

Hamurtekin E
Department of Pharmacology and Clinical Pharmacology, Faculty of Medicine, Uludag University, 16059 Görükle, Bursa, Turkey.
Bagdas D
No affiliation info available
Gurun MS
No affiliation info available

MeSH

Acute DiseaseAdrenergic alpha-AntagonistsAnalgesicsAnimalsBrainCytidine Diphosphate CholineDisease Models, AnimalEfferent PathwaysGABA AntagonistsInjections, IntraventricularMaleNarcotic AntagonistsNociceptorsPainPain MeasurementPain ThresholdRatsRats, Sprague-DawleyReceptors, Adrenergic, alphaReceptors, GABAReceptors, GABA-BReceptors, OpioidReceptors, SerotoninSerotonin Antagonists

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17531379

Citation

Hamurtekin, Emre, et al. "Possible Involvement of Supraspinal Opioid and GABA Receptors in CDP-choline-induced Antinociception in Acute Pain Models in Rats." Neuroscience Letters, vol. 420, no. 2, 2007, pp. 116-21.
Hamurtekin E, Bagdas D, Gurun MS. Possible involvement of supraspinal opioid and GABA receptors in CDP-choline-induced antinociception in acute pain models in rats. Neurosci Lett. 2007;420(2):116-21.
Hamurtekin, E., Bagdas, D., & Gurun, M. S. (2007). Possible involvement of supraspinal opioid and GABA receptors in CDP-choline-induced antinociception in acute pain models in rats. Neuroscience Letters, 420(2), 116-21.
Hamurtekin E, Bagdas D, Gurun MS. Possible Involvement of Supraspinal Opioid and GABA Receptors in CDP-choline-induced Antinociception in Acute Pain Models in Rats. Neurosci Lett. 2007 Jun 13;420(2):116-21. PubMed PMID: 17531379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Possible involvement of supraspinal opioid and GABA receptors in CDP-choline-induced antinociception in acute pain models in rats. AU - Hamurtekin,Emre, AU - Bagdas,Deniz, AU - Gurun,M Sibel, Y1 - 2007/04/29/ PY - 2007/04/04/received PY - 2007/04/24/revised PY - 2007/04/24/accepted PY - 2007/5/29/pubmed PY - 2007/8/11/medline PY - 2007/5/29/entrez SP - 116 EP - 21 JF - Neuroscience letters JO - Neurosci Lett VL - 420 IS - 2 N2 - Cytidine-5'-diphosphate choline (CDP-choline; citicoline) is an essential endogenous compound normally produced by the organism and is a source of cytidine and choline. Our recent studies on acute pain models demonstrate that intracerebroventricularly administered CDP-choline produces antinociception via supraspinal alpha-7 nicotinic acetylcholine receptors-mediated mechanism in rats. However, it remains to be elucidated which other supraspinal mechanisms are involved in the antinociceptive effect of CDP-choline. In this study, we investigated the role of the supraspinal opioidergic, GABAergic, alpha-adrenergic and serotonergic receptors in CDP-choline-induced antinociception. The antinociceptive effect of CDP-choline was evoked by the intracerebroventricular (i.c.v.) administration. Two different pain models were utilized: thermal paw withdrawal test and mechanical paw pressure test. The i.c.v. administration of CDP-choline (0.5, 1.0 and 2.0 micromol) produced dose-dependent antinociception. Non-specific opioid receptor antagonist naloxone (10 microg; i.c.v.) and GABA(B) receptor antagonist CGP-35348 (20 microg; i.c.v.) pretreatments inhibited the antinociceptive effects of CDP-choline (1.0 micromol; i.c.v.). In contrast, the alpha-1 adrenergic receptor antagonist prazosin (20 microg; i.c.v.), alpha-2 adrenergic receptor antagonist yohimbine (30 microg; i.c.v.) and non-specific serotonin receptor antagonist methysergide (20 microg; i.c.v.) pretreatments had no effect on CDP-choline-induced antinociception in the thermal paw withdrawal test and in the mechanical paw pressure test. Therefore, it can be postulated that i.c.v. administered CDP-choline exerts antinociceptive effect mediated by supraspinal opioid and GABA(B) receptors in acute pain models. Furthermore, supraspinal alpha-adrenergic and serotonergic receptors do not appear to be involved in the antinociceptive effect of CDP-choline. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/17531379/Possible_involvement_of_supraspinal_opioid_and_GABA_receptors_in_CDP_choline_induced_antinociception_in_acute_pain_models_in_rats_ DB - PRIME DP - Unbound Medicine ER -