TY - JOUR T1 - Mesoporous tungsten titanate as matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of biomolecules. AU - Shan,Zhe, AU - Han,Lu, AU - Yuan,Minjia, AU - Deng,Chunhui, AU - Zhao,Dongyuan, AU - Tu,Bo, AU - Yang,Pengyuan, Y1 - 2007/04/27/ PY - 2006/10/06/received PY - 2007/03/14/revised PY - 2007/04/20/accepted PY - 2007/5/29/pubmed PY - 2007/6/22/medline PY - 2007/5/29/entrez SP - 13 EP - 9 JF - Analytica chimica acta JO - Anal Chim Acta VL - 593 IS - 1 N2 - In this paper, mesoporous tungsten titanate (WTiO) with different nano-pore structures was utilized as matrix for the analysis of short peptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). Effect of characteristic features of mesoporous matrices on laser desorption/ionization process was investigated. Experiments showed that the ordered two-dimensional and three-dimensional mesoporous matrices were superior in performance to the non-ordered WTiO matrix. The dramatic enhancement of signal sensitivity by the ordered mesoporous matrices can be reasonably attributed to the ordered structure, which facilitated the understanding on structure-function relationship in mesoporous cavity for laser desorption process of adsorbed biomolecules. With the ordered mesoporous matrix, the short peptides are successfully detected. The presence of trace alkali metal salt effectively increased the analyte ion yields and the MALDI-TOFMS using the inorganic mesoporous matrices displayed a high salt tolerance. The developed technique also showed a satisfactory performance in peptide-mapping and amino-acid sequencing analysis. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17531819/Mesoporous_tungsten_titanate_as_matrix_for_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_analysis_of_biomolecules_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)00826-4 DB - PRIME DP - Unbound Medicine ER -