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Characterization of the CD3zeta, CD3gammadelta and CD3epsilon subunits of the T cell receptor complex in Atlantic salmon.
Dev Comp Immunol 2008; 32(1):26-35DC

Abstract

The CD3 subunits are essential components of the T cell receptor complex, transmitting signals to the inside of the cell. We report here cDNAs and corresponding genes encoding CD3zeta, CD3gammadelta and CD3epsilon in Atlantic salmon, and real-time RT-PCR analysis to reveal their tissue-specific expression. Salmon CD3zeta is the subunit that shows the highest sequence similarity to the mammalian counterparts, comprising of a short extracellular (EX) part, a transmembrane (TM) peptide and a long cytoplasmic (CY) tail with three immunoreceptor tyrosine-based activation motifs (ITAMs). The gene encoding CD3zeta in salmon has 7 exons. Salmon CD3gammadelta (a forerunner of CD3gamma and CD3delta in mammals) and CD3epsilon are related molecules each having an Ig-like EX domain, a TM peptide and a CY tail with one ITAM. Two distinct CD3gammadelta genes were found, each having 6 exons. The gene encoding CD3epsilon in salmon has 5 exons. RT-PCR also revealed a transcript from a degenerated CD3epsilon gene in salmon (Salmo salar) and brown trout (Salmo trutta). This pseudogene is located tail to tail to a CD3gammadelta gene in salmon and has a typical CD3epsilon gene structure with the exception of 1 extra exon. All the CD3 genes in salmon were most abundantly expressed in thymus but the expression of the CD3epsilon pseudogene was only a fraction of that from the intact CD3epsilon gene.

Authors+Show Affiliations

Department of Biology, University of Bergen, Norway.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17532043

Citation

Liu, Yun, et al. "Characterization of the CD3zeta, CD3gammadelta and CD3epsilon Subunits of the T Cell Receptor Complex in Atlantic Salmon." Developmental and Comparative Immunology, vol. 32, no. 1, 2008, pp. 26-35.
Liu Y, Moore L, Koppang EO, et al. Characterization of the CD3zeta, CD3gammadelta and CD3epsilon subunits of the T cell receptor complex in Atlantic salmon. Dev Comp Immunol. 2008;32(1):26-35.
Liu, Y., Moore, L., Koppang, E. O., & Hordvik, I. (2008). Characterization of the CD3zeta, CD3gammadelta and CD3epsilon subunits of the T cell receptor complex in Atlantic salmon. Developmental and Comparative Immunology, 32(1), pp. 26-35.
Liu Y, et al. Characterization of the CD3zeta, CD3gammadelta and CD3epsilon Subunits of the T Cell Receptor Complex in Atlantic Salmon. Dev Comp Immunol. 2008;32(1):26-35. PubMed PMID: 17532043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the CD3zeta, CD3gammadelta and CD3epsilon subunits of the T cell receptor complex in Atlantic salmon. AU - Liu,Yun, AU - Moore,Lindsey, AU - Koppang,Erling Olaf, AU - Hordvik,Ivar, Y1 - 2007/05/07/ PY - 2007/02/19/received PY - 2007/03/26/revised PY - 2007/03/30/accepted PY - 2007/5/29/pubmed PY - 2008/4/12/medline PY - 2007/5/29/entrez SP - 26 EP - 35 JF - Developmental and comparative immunology JO - Dev. Comp. Immunol. VL - 32 IS - 1 N2 - The CD3 subunits are essential components of the T cell receptor complex, transmitting signals to the inside of the cell. We report here cDNAs and corresponding genes encoding CD3zeta, CD3gammadelta and CD3epsilon in Atlantic salmon, and real-time RT-PCR analysis to reveal their tissue-specific expression. Salmon CD3zeta is the subunit that shows the highest sequence similarity to the mammalian counterparts, comprising of a short extracellular (EX) part, a transmembrane (TM) peptide and a long cytoplasmic (CY) tail with three immunoreceptor tyrosine-based activation motifs (ITAMs). The gene encoding CD3zeta in salmon has 7 exons. Salmon CD3gammadelta (a forerunner of CD3gamma and CD3delta in mammals) and CD3epsilon are related molecules each having an Ig-like EX domain, a TM peptide and a CY tail with one ITAM. Two distinct CD3gammadelta genes were found, each having 6 exons. The gene encoding CD3epsilon in salmon has 5 exons. RT-PCR also revealed a transcript from a degenerated CD3epsilon gene in salmon (Salmo salar) and brown trout (Salmo trutta). This pseudogene is located tail to tail to a CD3gammadelta gene in salmon and has a typical CD3epsilon gene structure with the exception of 1 extra exon. All the CD3 genes in salmon were most abundantly expressed in thymus but the expression of the CD3epsilon pseudogene was only a fraction of that from the intact CD3epsilon gene. SN - 0145-305X UR - https://www.unboundmedicine.com/medline/citation/17532043/Characterization_of_the_CD3zeta_CD3gammadelta_and_CD3epsilon_subunits_of_the_T_cell_receptor_complex_in_Atlantic_salmon_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0145-305X(07)00067-5 DB - PRIME DP - Unbound Medicine ER -