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Inactivation of E. coli, B. subtilis spores, and MS2, T4, and T7 phage using UV/H2O2 advanced oxidation.
J Hazard Mater. 2007 Jul 31; 146(3):479-86.JH

Abstract

The goal of this study was to evaluate the potential of an advanced oxidation process (AOP) for microbiocidal and virucidal inactivation. The viruses chosen for this study were bacteriophage MS2, T4, and T7. In addition, Bacillus subtilis spores and Escherichia coli were studied. By using H(2)O(2) in the presence of filtered ultraviolet (UV) irradiation (UV/H(2)O(2)) to generate wavelengths above 295nm, the direct UV photolysis disinfection mechanism was minimized, while disinfection by H(2)O(2) was also negligible. Virus T4 and E. coli in phosphate buffered saline (PBS) were sensitive to >295nm filtered UV irradiation (without H(2)O(2)), while MS2 was very resistant. Addition of H(2)O(2) at 25mg/l in the presence of filtered UV irradiation over a 15min reaction time did not result in any additional disinfection of virus T4, while an additional one log inactivation for T7 and 2.5 logs for MS2 were obtained. With E. coli, only a slight additional effect was observed when H(2)O(2) was added. B. subtilis spores did not show any inactivation at any of the conditions used in this study. The OH radical exposure (CT value) was calculated to present the relationship between the hydroxyl radical dose and microbial inactivation.

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Authors+Show Affiliations

Mamane H
School of Mechanical Engineering and Porter School of Environmental Studies, Tel-Aviv University, Tel-Aviv 69978, Israel. hadasmg@post.tau.ac.il
Shemer H
No affiliation info available
Linden KG
No affiliation info available

MeSH

Bacillus subtilisBacteriophagesDisinfectionEscherichia coliHydrogen PeroxideOxidantsOxidation-ReductionPhotolysisSpores, BacterialUltraviolet RaysWaste Disposal, FluidWater PollutantsWater Purification

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17532124

Citation

Mamane, Hadas, et al. "Inactivation of E. Coli, B. Subtilis Spores, and MS2, T4, and T7 Phage Using UV/H2O2 Advanced Oxidation." Journal of Hazardous Materials, vol. 146, no. 3, 2007, pp. 479-86.
Mamane H, Shemer H, Linden KG. Inactivation of E. coli, B. subtilis spores, and MS2, T4, and T7 phage using UV/H2O2 advanced oxidation. J Hazard Mater. 2007;146(3):479-86.
Mamane, H., Shemer, H., & Linden, K. G. (2007). Inactivation of E. coli, B. subtilis spores, and MS2, T4, and T7 phage using UV/H2O2 advanced oxidation. Journal of Hazardous Materials, 146(3), 479-86.
Mamane H, Shemer H, Linden KG. Inactivation of E. Coli, B. Subtilis Spores, and MS2, T4, and T7 Phage Using UV/H2O2 Advanced Oxidation. J Hazard Mater. 2007 Jul 31;146(3):479-86. PubMed PMID: 17532124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation of E. coli, B. subtilis spores, and MS2, T4, and T7 phage using UV/H2O2 advanced oxidation. AU - Mamane,Hadas, AU - Shemer,Hilla, AU - Linden,Karl G, Y1 - 2007/04/20/ PY - 2007/5/29/pubmed PY - 2007/9/28/medline PY - 2007/5/29/entrez SP - 479 EP - 86 JF - Journal of hazardous materials JO - J Hazard Mater VL - 146 IS - 3 N2 - The goal of this study was to evaluate the potential of an advanced oxidation process (AOP) for microbiocidal and virucidal inactivation. The viruses chosen for this study were bacteriophage MS2, T4, and T7. In addition, Bacillus subtilis spores and Escherichia coli were studied. By using H(2)O(2) in the presence of filtered ultraviolet (UV) irradiation (UV/H(2)O(2)) to generate wavelengths above 295nm, the direct UV photolysis disinfection mechanism was minimized, while disinfection by H(2)O(2) was also negligible. Virus T4 and E. coli in phosphate buffered saline (PBS) were sensitive to >295nm filtered UV irradiation (without H(2)O(2)), while MS2 was very resistant. Addition of H(2)O(2) at 25mg/l in the presence of filtered UV irradiation over a 15min reaction time did not result in any additional disinfection of virus T4, while an additional one log inactivation for T7 and 2.5 logs for MS2 were obtained. With E. coli, only a slight additional effect was observed when H(2)O(2) was added. B. subtilis spores did not show any inactivation at any of the conditions used in this study. The OH radical exposure (CT value) was calculated to present the relationship between the hydroxyl radical dose and microbial inactivation. SN - 0304-3894 UR - https://www.unboundmedicine.com/medline/citation/17532124/Inactivation_of_E__coli_B__subtilis_spores_and_MS2_T4_and_T7_phage_using_UV/H2O2_advanced_oxidation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3894(07)00534-1 DB - PRIME DP - Unbound Medicine ER -