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[Decade of genomics--methods for genome investigation in yeast Saccharomyces cerevisiae].
Postepy Biochem. 2006; 52(4):435-47.PB

Abstract

The last ten years, since yeast Saccharomyces cerevisiae genome was sequenced, brought a big impact in genome-wide techniques. The tenth anniversary of genomic era provokes the following resume: a lot of new methods were invented. The yeast strains libraries carrying transposon insertions, gene deletions or tagged proteins have been created. Using them, the phenotypes of gene deletions, as well as the biological activity, cellular localization and possible modifications of their protein products were elucidated. SAGE analysis and DNA microarray experiments showed gene expression profiles and allowed to build interaction networks of gene regulation. The two dimensional gels, mass spectrometry, protein arrays and two-hybrid system carry information about protein interactions, modifications, and biochemical activities. All these methods permit to increase the number of genes with known cellular functions. Moreover, testing these techniques on yeast S. cerevisiae--a model eukaryotic organism--opened the door for their usage in all other species.

Authors+Show Affiliations

Skoneczna A
Pracownia Mutagenezy i Reperacji DNA, Instytut Biochemii i Biofizyki PAN, Warszawa. ada@ibb.waw.pl

MeSH

Gene Expression ProfilingGenetic TechniquesGenome, FungalGenomicsLogical Observation Identifiers Names and CodesOligonucleotide Array Sequence AnalysisOpen Reading FramesProtein Interaction MappingProteomicsRetroelementsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTwo-Hybrid System Techniques

Pub Type(s)

Journal Article
Review

Language

pol

PubMed ID

17536513

Citation

Skoneczna, Adrianna. "[Decade of Genomics--methods for Genome Investigation in Yeast Saccharomyces Cerevisiae]." Postepy Biochemii, vol. 52, no. 4, 2006, pp. 435-47.
Skoneczna A. [Decade of genomics--methods for genome investigation in yeast Saccharomyces cerevisiae]. Postepy Biochem. 2006;52(4):435-47.
Skoneczna, A. (2006). [Decade of genomics--methods for genome investigation in yeast Saccharomyces cerevisiae]. Postepy Biochemii, 52(4), 435-47.
Skoneczna A. [Decade of Genomics--methods for Genome Investigation in Yeast Saccharomyces Cerevisiae]. Postepy Biochem. 2006;52(4):435-47. PubMed PMID: 17536513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Decade of genomics--methods for genome investigation in yeast Saccharomyces cerevisiae]. A1 - Skoneczna,Adrianna, PY - 2007/6/1/pubmed PY - 2007/7/27/medline PY - 2007/6/1/entrez SP - 435 EP - 47 JF - Postepy biochemii JO - Postepy Biochem VL - 52 IS - 4 N2 - The last ten years, since yeast Saccharomyces cerevisiae genome was sequenced, brought a big impact in genome-wide techniques. The tenth anniversary of genomic era provokes the following resume: a lot of new methods were invented. The yeast strains libraries carrying transposon insertions, gene deletions or tagged proteins have been created. Using them, the phenotypes of gene deletions, as well as the biological activity, cellular localization and possible modifications of their protein products were elucidated. SAGE analysis and DNA microarray experiments showed gene expression profiles and allowed to build interaction networks of gene regulation. The two dimensional gels, mass spectrometry, protein arrays and two-hybrid system carry information about protein interactions, modifications, and biochemical activities. All these methods permit to increase the number of genes with known cellular functions. Moreover, testing these techniques on yeast S. cerevisiae--a model eukaryotic organism--opened the door for their usage in all other species. SN - 0032-5422 UR - https://www.unboundmedicine.com/medline/citation/17536513/[Decade_of_genomics__methods_for_genome_investigation_in_yeast_Saccharomyces_cerevisiae]_ L2 - https://www.yeastgenome.org/reference/17536513 DB - PRIME DP - Unbound Medicine ER -