Tags

Type your tag names separated by a space and hit enter

Cytogenetic effects of grape extracts (Vitis vinifera) and polyphenols on mitomycin C-induced sister chromatid exchanges (SCEs) in human blood lymphocytes.
J Agric Food Chem. 2007 Jun 27; 55(13):5246-52.JA

Abstract

In the present study, the effects of extracts and polyphenol-rich fractions as well as monomer polyphenols identified in them, from both red and white grapes, on mitomycin C (MMC) induced sister chromatid exchanges (SCEs) in human peripheral blood lymphocytes were investigated. The grape extracts and two of the three polyphenol-rich fractions promoted MMC-induced SCEs at concentrations from 75 to 300 microg/mL. However, none of the extracts or fractions alone induced SCEs. Thus, these results suggest caution especially with regard to the use of grape extracts as dietary supplements. On the other hand, the fact that these extracts were not genotoxic alone may indicate a selective activity against genetically damaged cells. This is the first study regarding the clastogenic effects of grape extracts in human cells. Moreover, from the tested polyphenols, caffeic acid, gallic acid, and rutin hydrate enhanced MMC-induced clastogenicity, whereas ferulic acid, protocatechuic acid, (+)-catechin, (-)-epicatechin, and trans-resveratrol had no effect at concentrations between 5 and 100 microM. The differences in the chemical structures of the tested polyphenols may account for their differential effects on MMC clastogenicity.

Authors+Show Affiliations

Stagos D
Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou, GR-41221 Larissa, Greece.
Spanou C
No affiliation info available
Margariti M
No affiliation info available
Stathopoulos C
No affiliation info available
Mamuris Z
No affiliation info available
Kazantzoglou G
No affiliation info available
Magiatis P
No affiliation info available
Kouretas D
No affiliation info available

MeSH

AdultCells, CulturedFlavonoidsFruitHumansLymphocytesMitomycinPhenolsPlant ExtractsPolyphenolsSister Chromatid ExchangeVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17536817

Citation

Stagos, Dimitrios, et al. "Cytogenetic Effects of Grape Extracts (Vitis Vinifera) and Polyphenols On Mitomycin C-induced Sister Chromatid Exchanges (SCEs) in Human Blood Lymphocytes." Journal of Agricultural and Food Chemistry, vol. 55, no. 13, 2007, pp. 5246-52.
Stagos D, Spanou C, Margariti M, et al. Cytogenetic effects of grape extracts (Vitis vinifera) and polyphenols on mitomycin C-induced sister chromatid exchanges (SCEs) in human blood lymphocytes. J Agric Food Chem. 2007;55(13):5246-52.
Stagos, D., Spanou, C., Margariti, M., Stathopoulos, C., Mamuris, Z., Kazantzoglou, G., Magiatis, P., & Kouretas, D. (2007). Cytogenetic effects of grape extracts (Vitis vinifera) and polyphenols on mitomycin C-induced sister chromatid exchanges (SCEs) in human blood lymphocytes. Journal of Agricultural and Food Chemistry, 55(13), 5246-52.
Stagos D, et al. Cytogenetic Effects of Grape Extracts (Vitis Vinifera) and Polyphenols On Mitomycin C-induced Sister Chromatid Exchanges (SCEs) in Human Blood Lymphocytes. J Agric Food Chem. 2007 Jun 27;55(13):5246-52. PubMed PMID: 17536817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytogenetic effects of grape extracts (Vitis vinifera) and polyphenols on mitomycin C-induced sister chromatid exchanges (SCEs) in human blood lymphocytes. AU - Stagos,Dimitrios, AU - Spanou,Chrysa, AU - Margariti,Maria, AU - Stathopoulos,Constantinos, AU - Mamuris,Zissis, AU - Kazantzoglou,Georgios, AU - Magiatis,Prokopios, AU - Kouretas,Demetrios, Y1 - 2007/05/31/ PY - 2007/6/1/pubmed PY - 2007/8/19/medline PY - 2007/6/1/entrez SP - 5246 EP - 52 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 13 N2 - In the present study, the effects of extracts and polyphenol-rich fractions as well as monomer polyphenols identified in them, from both red and white grapes, on mitomycin C (MMC) induced sister chromatid exchanges (SCEs) in human peripheral blood lymphocytes were investigated. The grape extracts and two of the three polyphenol-rich fractions promoted MMC-induced SCEs at concentrations from 75 to 300 microg/mL. However, none of the extracts or fractions alone induced SCEs. Thus, these results suggest caution especially with regard to the use of grape extracts as dietary supplements. On the other hand, the fact that these extracts were not genotoxic alone may indicate a selective activity against genetically damaged cells. This is the first study regarding the clastogenic effects of grape extracts in human cells. Moreover, from the tested polyphenols, caffeic acid, gallic acid, and rutin hydrate enhanced MMC-induced clastogenicity, whereas ferulic acid, protocatechuic acid, (+)-catechin, (-)-epicatechin, and trans-resveratrol had no effect at concentrations between 5 and 100 microM. The differences in the chemical structures of the tested polyphenols may account for their differential effects on MMC clastogenicity. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17536817/Cytogenetic_effects_of_grape_extracts__Vitis_vinifera__and_polyphenols_on_mitomycin_C_induced_sister_chromatid_exchanges__SCEs__in_human_blood_lymphocytes_ DB - PRIME DP - Unbound Medicine ER -