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Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis.
Eur J Pharmacol. 2007 Jul 30; 568(1-3):213-21.EJ

Abstract

Tanshinone IIA (tan), a derivative of phenanthrenequinone, is one of the key components of Salvia miltiorrhiza Bunge. Previous reports showed that tan inhibited the apoptosis of cultured PC12 cells induced by serum withdrawal or ethanol. However, whether tan has a cardioprotective effect against apoptosis remains unknown. In this study, we investigated the effects of tan on cardiac myocyte apoptosis induced both by in vitro incubation of neonatal rat ventricular myocytes with H(2)O(2) and by in vivo occlusion followed by reperfusion of the left anterior descending coronary artery in adult rats. In vitro, as revealed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay, treatment with tan prior to H(2)O(2) exposure significantly increased cell viability. Tan also markedly inhibited H(2)O(2)-induced cardiomyocyte apoptosis, as detected by ladder-pattern fragmentation of genomic DNA, chromatin condensation, and hypodioloid DNA content. In vivo, tan significantly inhibited ischemia/reperfusion-induced cardiomyocyte apoptosis by attenuating morphological changes and reducing the percentage of terminal transferase dUTP nick end-labeling (TUNEL)-positive myocytes and caspase-3 cleavage. These effects of tan were associated with an increased ratio of Bcl-2 to Bax protein in cardiomyocytes, an elevation of serum superoxide dismutase (SOD) activity and a decrease in serum malondialdehyde (MDA) level. Taken together, these data for the first time provide convincing evidence that tan protects cardiac myocytes against oxidative stress-induced apoptosis. The in vivo protection is mediated by increased scavenging of oxygen free radicals, prevention of lipid peroxidation and upregulation of the Bcl-2/Bax ratio.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Sun Yat-sen University, Guangzhou, 510080, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17537428

Citation

Fu, Jiajia, et al. "Tanshinone IIA Protects Cardiac Myocytes Against Oxidative Stress-triggered Damage and Apoptosis." European Journal of Pharmacology, vol. 568, no. 1-3, 2007, pp. 213-21.
Fu J, Huang H, Liu J, et al. Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis. Eur J Pharmacol. 2007;568(1-3):213-21.
Fu, J., Huang, H., Liu, J., Pi, R., Chen, J., & Liu, P. (2007). Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis. European Journal of Pharmacology, 568(1-3), 213-21.
Fu J, et al. Tanshinone IIA Protects Cardiac Myocytes Against Oxidative Stress-triggered Damage and Apoptosis. Eur J Pharmacol. 2007 Jul 30;568(1-3):213-21. PubMed PMID: 17537428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tanshinone IIA protects cardiac myocytes against oxidative stress-triggered damage and apoptosis. AU - Fu,Jiajia, AU - Huang,Heqing, AU - Liu,Jiajun, AU - Pi,Rongbiao, AU - Chen,Jianwen, AU - Liu,Peiqing, Y1 - 2007/04/27/ PY - 2006/07/23/received PY - 2007/04/03/revised PY - 2007/04/04/accepted PY - 2007/6/1/pubmed PY - 2007/10/20/medline PY - 2007/6/1/entrez SP - 213 EP - 21 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 568 IS - 1-3 N2 - Tanshinone IIA (tan), a derivative of phenanthrenequinone, is one of the key components of Salvia miltiorrhiza Bunge. Previous reports showed that tan inhibited the apoptosis of cultured PC12 cells induced by serum withdrawal or ethanol. However, whether tan has a cardioprotective effect against apoptosis remains unknown. In this study, we investigated the effects of tan on cardiac myocyte apoptosis induced both by in vitro incubation of neonatal rat ventricular myocytes with H(2)O(2) and by in vivo occlusion followed by reperfusion of the left anterior descending coronary artery in adult rats. In vitro, as revealed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay, treatment with tan prior to H(2)O(2) exposure significantly increased cell viability. Tan also markedly inhibited H(2)O(2)-induced cardiomyocyte apoptosis, as detected by ladder-pattern fragmentation of genomic DNA, chromatin condensation, and hypodioloid DNA content. In vivo, tan significantly inhibited ischemia/reperfusion-induced cardiomyocyte apoptosis by attenuating morphological changes and reducing the percentage of terminal transferase dUTP nick end-labeling (TUNEL)-positive myocytes and caspase-3 cleavage. These effects of tan were associated with an increased ratio of Bcl-2 to Bax protein in cardiomyocytes, an elevation of serum superoxide dismutase (SOD) activity and a decrease in serum malondialdehyde (MDA) level. Taken together, these data for the first time provide convincing evidence that tan protects cardiac myocytes against oxidative stress-induced apoptosis. The in vivo protection is mediated by increased scavenging of oxygen free radicals, prevention of lipid peroxidation and upregulation of the Bcl-2/Bax ratio. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/17537428/Tanshinone_IIA_protects_cardiac_myocytes_against_oxidative_stress_triggered_damage_and_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)00469-4 DB - PRIME DP - Unbound Medicine ER -