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San-Huang-Xie-Xin-Tang inhibits Helicobacter pylori-induced inflammation in human gastric epithelial AGS cells.
J Ethnopharmacol. 2007 Jul 25; 112(3):537-44.JE

Abstract

Helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration, gastric lymphoma and gastric cancer. Certain herbal remedies have been used to treat gastric disease. In this study, we examined the anti-inflammatory effect of San-Huang-Xie-Xin-Tang (SHXT) and its main component baicalin on Helicobacter pylori-infected human gastric epithelial AGS cell. AGS cells were treated with Helicobacter pylori at a bacterium/cell ratio of 300:1. mRNA expression and protein levels were determined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and western blot analysis, respectively. Interleukin-8 (IL-8) level and the translocation of nuclear factor kappa B (NF-kappaB) were measured by enzyme-linked immunosorbent assay (ELISA) and enzyme-linked DNA-protein interaction assay (ELDIA), respectively. Nitric oxide production was measured by Griess reagent. We found that SHXT and baicalin inhibited Helicobacter pylori-induced cyclooxygenase-2 (COX-2) enhancement and IkappaBalpha degradation in both mRNA and protein levels. SHXT and baicalin also inhibited Helicobacter pylori-induced inducible nitric oxide synthase (iNOS) and IL-8 mRNA expression, and decreased NO and IL-8 production. Furthermore, SHXT and baicalin inhibited nuclear translocation of p50 subunit of NF-kappaB in Helicobacter pylori-infected AGS cells. Based on the above findings, SHXT and baicalin might exert anti-inflammatory and gastroprotective effects in Helicobacter pylori-induced gastric inflammation.

Authors+Show Affiliations

Department and Graduate Institute of Pharmacology, College of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17537603

Citation

Shih, Yu-Tzu, et al. "San-Huang-Xie-Xin-Tang Inhibits Helicobacter Pylori-induced Inflammation in Human Gastric Epithelial AGS Cells." Journal of Ethnopharmacology, vol. 112, no. 3, 2007, pp. 537-44.
Shih YT, Wu DC, Liu CM, et al. San-Huang-Xie-Xin-Tang inhibits Helicobacter pylori-induced inflammation in human gastric epithelial AGS cells. J Ethnopharmacol. 2007;112(3):537-44.
Shih, Y. T., Wu, D. C., Liu, C. M., Yang, Y. C., Chen, I. J., & Lo, Y. C. (2007). San-Huang-Xie-Xin-Tang inhibits Helicobacter pylori-induced inflammation in human gastric epithelial AGS cells. Journal of Ethnopharmacology, 112(3), 537-44.
Shih YT, et al. San-Huang-Xie-Xin-Tang Inhibits Helicobacter Pylori-induced Inflammation in Human Gastric Epithelial AGS Cells. J Ethnopharmacol. 2007 Jul 25;112(3):537-44. PubMed PMID: 17537603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - San-Huang-Xie-Xin-Tang inhibits Helicobacter pylori-induced inflammation in human gastric epithelial AGS cells. AU - Shih,Yu-Tzu, AU - Wu,Deng-Chyang, AU - Liu,Chi-Ming, AU - Yang,Yuan-Chieh, AU - Chen,Ing-Jun, AU - Lo,Yi-Ching, Y1 - 2007/04/27/ PY - 2006/10/11/received PY - 2007/01/24/revised PY - 2007/04/20/accepted PY - 2007/6/1/pubmed PY - 2007/12/13/medline PY - 2007/6/1/entrez SP - 537 EP - 44 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 112 IS - 3 N2 - Helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration, gastric lymphoma and gastric cancer. Certain herbal remedies have been used to treat gastric disease. In this study, we examined the anti-inflammatory effect of San-Huang-Xie-Xin-Tang (SHXT) and its main component baicalin on Helicobacter pylori-infected human gastric epithelial AGS cell. AGS cells were treated with Helicobacter pylori at a bacterium/cell ratio of 300:1. mRNA expression and protein levels were determined by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and western blot analysis, respectively. Interleukin-8 (IL-8) level and the translocation of nuclear factor kappa B (NF-kappaB) were measured by enzyme-linked immunosorbent assay (ELISA) and enzyme-linked DNA-protein interaction assay (ELDIA), respectively. Nitric oxide production was measured by Griess reagent. We found that SHXT and baicalin inhibited Helicobacter pylori-induced cyclooxygenase-2 (COX-2) enhancement and IkappaBalpha degradation in both mRNA and protein levels. SHXT and baicalin also inhibited Helicobacter pylori-induced inducible nitric oxide synthase (iNOS) and IL-8 mRNA expression, and decreased NO and IL-8 production. Furthermore, SHXT and baicalin inhibited nuclear translocation of p50 subunit of NF-kappaB in Helicobacter pylori-infected AGS cells. Based on the above findings, SHXT and baicalin might exert anti-inflammatory and gastroprotective effects in Helicobacter pylori-induced gastric inflammation. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/17537603/San_Huang_Xie_Xin_Tang_inhibits_Helicobacter_pylori_induced_inflammation_in_human_gastric_epithelial_AGS_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(07)00214-0 DB - PRIME DP - Unbound Medicine ER -