Tags

Type your tag names separated by a space and hit enter

Abilities of APTIMA, AMPLICOR, and ProbeTec assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in PreservCyt ThinPrep Liquid-based Pap samples.
J Clin Microbiol. 2007 Aug; 45(8):2355-8.JC

Abstract

Infections with Chlamydia trachomatis and Neisseria gonorrhoeae are often asymptomatic. Liquid-based Pap (L-Pap) screening may provide samples for testing by commercial assays. Women attending a health clinic or a street youth clinic had a PreservCyt ThinPrep sample and a cervical swab (CS) collected. The L-Pap sample was tested for cytopathology; then 1 ml was transferred to an L-Pap specimen transfer tube for testing by the Gen-Probe APTIMA assays (APTIMA Combo 2 [AC2], APTIMA C. trachomatis [ACT], and APTIMA N. gonorrhoeae [AGC]). The residual L-Pap sample was tested for C. trachomatis and N. gonorrhoeae using Roche AMPLICOR (AMP) and Becton Dickinson ProbeTec (PT). The CS was tested by AC2. A patient was considered infected if two specimens were positive or if a single specimen was positive in two tests. The prevalence of infection was 10% (29/290) for C. trachomatis and 2.4% (7/290) for N. gonorrhoeae. Most of the positive patients had specimens that were reactive in all assays (20/29 for C. trachomatis; 6/7 for N. gonorrhoeae). Four patients had double infections. The sensitivities and specificities of the various tests for the specimens tested were as follows. For C. trachomatis on L-Pap, sensitivity and specificity were 100 and 98.1%, respectively, for ACT, 93.1 and 98.8% for AC2, 86.2 and 91.2% for AMP, and 72.4 and 92.7% for PT. For N. gonorrhoeae on L-Pap, sensitivity and specificity were 100% for both AGC and AC2, 85.7 and 100% for AMP, and 85.7 and 100% for PT. For AC2 with CSs, sensitivity and specificity were 93.1 and 98.5%, respectively, for C. trachomatis, and both were 100% for N. gonorrhoeae. There were significant differences in sensitivity and specificity (P < 0.001). The APTIMA assays were more sensitive and specific than AMP or PT for detecting women's C. trachomatis and/or N. gonorrhoeae infections by testing ThinPrep samples.

Authors+Show Affiliations

St. Joseph's Healthcare/McMaster University, Toronto, Ontario, Canada. chernesk@mcmaster.caNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

17537943

Citation

Chernesky, M, et al. "Abilities of APTIMA, AMPLICOR, and ProbeTec Assays to Detect Chlamydia Trachomatis and Neisseria Gonorrhoeae in PreservCyt ThinPrep Liquid-based Pap Samples." Journal of Clinical Microbiology, vol. 45, no. 8, 2007, pp. 2355-8.
Chernesky M, Jang D, Portillo E, et al. Abilities of APTIMA, AMPLICOR, and ProbeTec assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in PreservCyt ThinPrep Liquid-based Pap samples. J Clin Microbiol. 2007;45(8):2355-8.
Chernesky, M., Jang, D., Portillo, E., Chong, S., Smieja, M., Luinstra, K., Petrich, A., Macritchie, C., Ewert, R., Hayhoe, B., Sarabia, A., & Thompson, F. (2007). Abilities of APTIMA, AMPLICOR, and ProbeTec assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in PreservCyt ThinPrep Liquid-based Pap samples. Journal of Clinical Microbiology, 45(8), 2355-8.
Chernesky M, et al. Abilities of APTIMA, AMPLICOR, and ProbeTec Assays to Detect Chlamydia Trachomatis and Neisseria Gonorrhoeae in PreservCyt ThinPrep Liquid-based Pap Samples. J Clin Microbiol. 2007;45(8):2355-8. PubMed PMID: 17537943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abilities of APTIMA, AMPLICOR, and ProbeTec assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in PreservCyt ThinPrep Liquid-based Pap samples. AU - Chernesky,M, AU - Jang,D, AU - Portillo,E, AU - Chong,S, AU - Smieja,M, AU - Luinstra,K, AU - Petrich,A, AU - Macritchie,C, AU - Ewert,R, AU - Hayhoe,B, AU - Sarabia,A, AU - Thompson,F, Y1 - 2007/05/30/ PY - 2007/6/1/pubmed PY - 2007/11/7/medline PY - 2007/6/1/entrez SP - 2355 EP - 8 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 45 IS - 8 N2 - Infections with Chlamydia trachomatis and Neisseria gonorrhoeae are often asymptomatic. Liquid-based Pap (L-Pap) screening may provide samples for testing by commercial assays. Women attending a health clinic or a street youth clinic had a PreservCyt ThinPrep sample and a cervical swab (CS) collected. The L-Pap sample was tested for cytopathology; then 1 ml was transferred to an L-Pap specimen transfer tube for testing by the Gen-Probe APTIMA assays (APTIMA Combo 2 [AC2], APTIMA C. trachomatis [ACT], and APTIMA N. gonorrhoeae [AGC]). The residual L-Pap sample was tested for C. trachomatis and N. gonorrhoeae using Roche AMPLICOR (AMP) and Becton Dickinson ProbeTec (PT). The CS was tested by AC2. A patient was considered infected if two specimens were positive or if a single specimen was positive in two tests. The prevalence of infection was 10% (29/290) for C. trachomatis and 2.4% (7/290) for N. gonorrhoeae. Most of the positive patients had specimens that were reactive in all assays (20/29 for C. trachomatis; 6/7 for N. gonorrhoeae). Four patients had double infections. The sensitivities and specificities of the various tests for the specimens tested were as follows. For C. trachomatis on L-Pap, sensitivity and specificity were 100 and 98.1%, respectively, for ACT, 93.1 and 98.8% for AC2, 86.2 and 91.2% for AMP, and 72.4 and 92.7% for PT. For N. gonorrhoeae on L-Pap, sensitivity and specificity were 100% for both AGC and AC2, 85.7 and 100% for AMP, and 85.7 and 100% for PT. For AC2 with CSs, sensitivity and specificity were 93.1 and 98.5%, respectively, for C. trachomatis, and both were 100% for N. gonorrhoeae. There were significant differences in sensitivity and specificity (P < 0.001). The APTIMA assays were more sensitive and specific than AMP or PT for detecting women's C. trachomatis and/or N. gonorrhoeae infections by testing ThinPrep samples. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/17537943/Abilities_of_APTIMA_AMPLICOR_and_ProbeTec_assays_to_detect_Chlamydia_trachomatis_and_Neisseria_gonorrhoeae_in_PreservCyt_ThinPrep_Liquid_based_Pap_samples_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&amp;pmid=17537943 DB - PRIME DP - Unbound Medicine ER -