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Analysis of acrylamide in water using a coevaporation preparative step and isotope dilution liquid chromatography tandem mass spectrometry.
Anal Chem. 2007 Jul 01; 79(13):5093-6.AC

Abstract

Acrylamide is a probable human carcinogen, and the drinking water quality guideline for this compound is 0.5 mg/L. However, analysis of this compound in water is difficult because of its very high water solubility, which limits the efficiency of sample preconcentration prior to analysis. We developed a robust and sensitive analytical method for the determination of trace quantities of acrylamide in samples of water using a novel preparative technique and isotope dilution liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization as the ion source (LC-APCI-MS/MS). The preparative method involves coevaporation of acrylamide with water at pH 10 using a rotary evaporator, followed by acidification to pH 3.0 and concentration of the sample prior to analysis by LC-APCI-MS/MS. To compensate for the loss of the analyte during sample preparation and signal suppression due to interference from the sample matrix, isotope dilution with acrylamide-d3 was used for quantitation. Using this method, analyte recoveries ranged from 74 to 103% for acrylamide spiked into water at a concentration of 0.4 ng/mL. The limit of detection and limit of quantification (LOQ) for acrylamide in water were 0.02 and 0.06 ng/mL, respectively. This method was successfully applied to determine trace levels of acrylamide in samples of river water and in runoff from an agricultural field to which municipal biosolids (i.e., sludge) had been applied. Concentrations of acrylamide in these samples ranged from <LOQ to 0.16 microg/L.

Authors+Show Affiliations

Worsfold Water Quality Center, Trent University, Peterborough, Ontario, K9J 7B8, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17539600

Citation

Chu, Shaogang, and Chris D. Metcalfe. "Analysis of Acrylamide in Water Using a Coevaporation Preparative Step and Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry." Analytical Chemistry, vol. 79, no. 13, 2007, pp. 5093-6.
Chu S, Metcalfe CD. Analysis of acrylamide in water using a coevaporation preparative step and isotope dilution liquid chromatography tandem mass spectrometry. Anal Chem. 2007;79(13):5093-6.
Chu, S., & Metcalfe, C. D. (2007). Analysis of acrylamide in water using a coevaporation preparative step and isotope dilution liquid chromatography tandem mass spectrometry. Analytical Chemistry, 79(13), 5093-6.
Chu S, Metcalfe CD. Analysis of Acrylamide in Water Using a Coevaporation Preparative Step and Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry. Anal Chem. 2007 Jul 1;79(13):5093-6. PubMed PMID: 17539600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of acrylamide in water using a coevaporation preparative step and isotope dilution liquid chromatography tandem mass spectrometry. AU - Chu,Shaogang, AU - Metcalfe,Chris D, Y1 - 2007/06/01/ PY - 2007/6/2/pubmed PY - 2007/9/8/medline PY - 2007/6/2/entrez SP - 5093 EP - 6 JF - Analytical chemistry JO - Anal Chem VL - 79 IS - 13 N2 - Acrylamide is a probable human carcinogen, and the drinking water quality guideline for this compound is 0.5 mg/L. However, analysis of this compound in water is difficult because of its very high water solubility, which limits the efficiency of sample preconcentration prior to analysis. We developed a robust and sensitive analytical method for the determination of trace quantities of acrylamide in samples of water using a novel preparative technique and isotope dilution liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization as the ion source (LC-APCI-MS/MS). The preparative method involves coevaporation of acrylamide with water at pH 10 using a rotary evaporator, followed by acidification to pH 3.0 and concentration of the sample prior to analysis by LC-APCI-MS/MS. To compensate for the loss of the analyte during sample preparation and signal suppression due to interference from the sample matrix, isotope dilution with acrylamide-d3 was used for quantitation. Using this method, analyte recoveries ranged from 74 to 103% for acrylamide spiked into water at a concentration of 0.4 ng/mL. The limit of detection and limit of quantification (LOQ) for acrylamide in water were 0.02 and 0.06 ng/mL, respectively. This method was successfully applied to determine trace levels of acrylamide in samples of river water and in runoff from an agricultural field to which municipal biosolids (i.e., sludge) had been applied. Concentrations of acrylamide in these samples ranged from <LOQ to 0.16 microg/L. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/17539600/Analysis_of_acrylamide_in_water_using_a_coevaporation_preparative_step_and_isotope_dilution_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://doi.org/10.1021/ac0623179 DB - PRIME DP - Unbound Medicine ER -