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Antigen-specific oligoclonal bands in cerebrospinal fluid and serum from patients with anti-amphiphysin- and anti-CV2/CRMP5 associated paraneoplastic neurological syndromes.
Eur J Neurol. 2007 Jun; 14(6):650-3.EJ

Abstract

Using isoelectric focusing and affinity blotting employing paraneoplastic recombinant antigens, we investigated cerebrospinal fluid (CSF) and sera from three patients with positive anti-CV2/CRMP5- and one patient with positive anti-amphiphysin serology. CSF and sera were previously adjusted to total IgG concentrations of 20 mg/l. All patients suffered from paraneoplastic neurological syndromes (PNS) with predominant involvement of the central nervous system (CNS). Using affinity blot preloaded with paraneoplastic antigen, we detected in three of four patients more or stronger specific oligoclonal bands (OCB) in the CSF than in the corresponding serum, providing qualitative evidence of antigen specific intrathecal antibody synthesis. These results are in line with previous studies demonstrating specific OCB predominantly in CSF from patients with anti-Hu-, anti-Yo- and anti-Ri-associated PNS, supporting the hypothesis of autoimmunity in the pathogenesis of PNS. One patient harboured extensive anti-amphiphysin specific OCB, although OCB of total IgG could not be detected, indicating a higher sensitivity for detection of intrathecal antibody synthesis of the affinity blot preloaded with the paraneoplastic antigen, compared with investigation of total IgG OCB. These results could have implications concerning pathophysiological autoimmune aspects in other inflammatory diseases of CNS associated with total IgG OCB, provided that the target antigen is known.

Authors+Show Affiliations

Neurologische Klinik und Poliklinik der Albert-Ludwigs-Universitaet Freiburg, Freiburg, Germany.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17539943

Citation

Stich, O, and S Rauer. "Antigen-specific Oligoclonal Bands in Cerebrospinal Fluid and Serum From Patients With Anti-amphiphysin- and anti-CV2/CRMP5 Associated Paraneoplastic Neurological Syndromes." European Journal of Neurology, vol. 14, no. 6, 2007, pp. 650-3.
Stich O, Rauer S. Antigen-specific oligoclonal bands in cerebrospinal fluid and serum from patients with anti-amphiphysin- and anti-CV2/CRMP5 associated paraneoplastic neurological syndromes. Eur J Neurol. 2007;14(6):650-3.
Stich, O., & Rauer, S. (2007). Antigen-specific oligoclonal bands in cerebrospinal fluid and serum from patients with anti-amphiphysin- and anti-CV2/CRMP5 associated paraneoplastic neurological syndromes. European Journal of Neurology, 14(6), 650-3.
Stich O, Rauer S. Antigen-specific Oligoclonal Bands in Cerebrospinal Fluid and Serum From Patients With Anti-amphiphysin- and anti-CV2/CRMP5 Associated Paraneoplastic Neurological Syndromes. Eur J Neurol. 2007;14(6):650-3. PubMed PMID: 17539943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antigen-specific oligoclonal bands in cerebrospinal fluid and serum from patients with anti-amphiphysin- and anti-CV2/CRMP5 associated paraneoplastic neurological syndromes. AU - Stich,O, AU - Rauer,S, PY - 2007/6/2/pubmed PY - 2007/7/11/medline PY - 2007/6/2/entrez SP - 650 EP - 3 JF - European journal of neurology JO - Eur. J. Neurol. VL - 14 IS - 6 N2 - Using isoelectric focusing and affinity blotting employing paraneoplastic recombinant antigens, we investigated cerebrospinal fluid (CSF) and sera from three patients with positive anti-CV2/CRMP5- and one patient with positive anti-amphiphysin serology. CSF and sera were previously adjusted to total IgG concentrations of 20 mg/l. All patients suffered from paraneoplastic neurological syndromes (PNS) with predominant involvement of the central nervous system (CNS). Using affinity blot preloaded with paraneoplastic antigen, we detected in three of four patients more or stronger specific oligoclonal bands (OCB) in the CSF than in the corresponding serum, providing qualitative evidence of antigen specific intrathecal antibody synthesis. These results are in line with previous studies demonstrating specific OCB predominantly in CSF from patients with anti-Hu-, anti-Yo- and anti-Ri-associated PNS, supporting the hypothesis of autoimmunity in the pathogenesis of PNS. One patient harboured extensive anti-amphiphysin specific OCB, although OCB of total IgG could not be detected, indicating a higher sensitivity for detection of intrathecal antibody synthesis of the affinity blot preloaded with the paraneoplastic antigen, compared with investigation of total IgG OCB. These results could have implications concerning pathophysiological autoimmune aspects in other inflammatory diseases of CNS associated with total IgG OCB, provided that the target antigen is known. SN - 1468-1331 UR - https://www.unboundmedicine.com/medline/citation/17539943/Antigen_specific_oligoclonal_bands_in_cerebrospinal_fluid_and_serum_from_patients_with_anti_amphiphysin__and_anti_CV2/CRMP5_associated_paraneoplastic_neurological_syndromes_ L2 - https://doi.org/10.1111/j.1468-1331.2007.01802.x DB - PRIME DP - Unbound Medicine ER -