Tags

Type your tag names separated by a space and hit enter

Asymmetric self-renewal and commitment of satellite stem cells in muscle.
Cell. 2007 Jun 01; 129(5):999-1010.Cell

Abstract

Satellite cells play a central role in mediating the growth and regeneration of skeletal muscle. However, whether satellite cells are stem cells, committed progenitors, or dedifferentiated myoblasts has remained unclear. Using Myf5-Cre and ROSA26-YFP Cre-reporter alleles, we observed that in vivo 10% of sublaminar Pax7-expressing satellite cells have never expressed Myf5. Moreover, we found that Pax7(+)/Myf5(-) satellite cells gave rise to Pax7(+)/Myf5(+) satellite cells through apical-basal oriented divisions that asymmetrically generated a basal Pax7(+)/Myf5(-) and an apical Pax7(+)/Myf5(+) cells. Prospective isolation and transplantation into muscle revealed that whereas Pax7(+)/Myf5(+) cells exhibited precocious differentiation, Pax7(+)/Myf5(-) cells extensively contributed to the satellite cell reservoir throughout the injected muscle. Therefore, we conclude that satellite cells are a heterogeneous population composed of stem cells and committed progenitors. These results provide critical insights into satellite cell biology and open new avenues for therapeutic treatment of neuromuscular diseases.

Authors+Show Affiliations

The Sprott Center for Stem Cell Research, Ottawa Health Research Institute, Molecular Medicine Program, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17540178

Citation

Kuang, Shihuan, et al. "Asymmetric Self-renewal and Commitment of Satellite Stem Cells in Muscle." Cell, vol. 129, no. 5, 2007, pp. 999-1010.
Kuang S, Kuroda K, Le Grand F, et al. Asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell. 2007;129(5):999-1010.
Kuang, S., Kuroda, K., Le Grand, F., & Rudnicki, M. A. (2007). Asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell, 129(5), 999-1010.
Kuang S, et al. Asymmetric Self-renewal and Commitment of Satellite Stem Cells in Muscle. Cell. 2007 Jun 1;129(5):999-1010. PubMed PMID: 17540178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Asymmetric self-renewal and commitment of satellite stem cells in muscle. AU - Kuang,Shihuan, AU - Kuroda,Kazuki, AU - Le Grand,Fabien, AU - Rudnicki,Michael A, PY - 2006/06/14/received PY - 2007/02/07/revised PY - 2007/03/07/accepted PY - 2007/6/2/pubmed PY - 2007/7/19/medline PY - 2007/6/2/entrez SP - 999 EP - 1010 JF - Cell JO - Cell VL - 129 IS - 5 N2 - Satellite cells play a central role in mediating the growth and regeneration of skeletal muscle. However, whether satellite cells are stem cells, committed progenitors, or dedifferentiated myoblasts has remained unclear. Using Myf5-Cre and ROSA26-YFP Cre-reporter alleles, we observed that in vivo 10% of sublaminar Pax7-expressing satellite cells have never expressed Myf5. Moreover, we found that Pax7(+)/Myf5(-) satellite cells gave rise to Pax7(+)/Myf5(+) satellite cells through apical-basal oriented divisions that asymmetrically generated a basal Pax7(+)/Myf5(-) and an apical Pax7(+)/Myf5(+) cells. Prospective isolation and transplantation into muscle revealed that whereas Pax7(+)/Myf5(+) cells exhibited precocious differentiation, Pax7(+)/Myf5(-) cells extensively contributed to the satellite cell reservoir throughout the injected muscle. Therefore, we conclude that satellite cells are a heterogeneous population composed of stem cells and committed progenitors. These results provide critical insights into satellite cell biology and open new avenues for therapeutic treatment of neuromuscular diseases. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/17540178/Asymmetric_self_renewal_and_commitment_of_satellite_stem_cells_in_muscle_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(07)00513-2 DB - PRIME DP - Unbound Medicine ER -