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Antioxidative and antiproliferative properties of selected barley (Hordeum vulgarae L.) cultivars and their potential for inhibition of low-density lipoprotein (LDL) cholesterol oxidation.
J Agric Food Chem. 2007 Jun 27; 55(13):5018-24.JA

Abstract

Aqueous methanolic extracts of whole kernels from six different barley cultivars, namely, Falcon, AC Metcalfe, Tyto, Tercel, Phoenix, and Peregrine, were examined for their total phenolic content (TPC), oxygen radical scavenging capacity (ORACFL), hydroxyl radical scavenging capacity (HORACFL), potency in prevention of lipid oxidation using the Rancimat method, efficacy in inhibition of Cu(II)-induced human LDL cholesterol oxidation, and antiproliferative activities using Caco-2 colorectal adenocarcinoma cell line. Total phenolic content as measured by Folin-Ciocalteu's method ranged from 0.68 to 1.19 mg of ferulic acid equiv/g of defatted material, whereas ORACFL and HORACFL values were 11.28-19.10 and 9.06-12.99 micromol of Trolox equiv/g of defatted material, respectively. Protection factor (PF), a measure of the effect of extracts on the prevention of oxidation of stripped corn oil as measured by Rancimat, ranged from 0.97 to 1.59. Furthermore, barley extracts showed 19.64-33.93% inhibition against Cu(II)-induced human LDL cholesterol oxidation at a final concentration of 0.02 mg/mL. The proliferation of Caco-2 colon cancer cells was significantly (p < 0.05) inhibited in a dose-dependent fashion in the presence of all barley extracts tested at the end of the day 4 of incubation. At the end of day 4, barley extracts rendered 29.3-51.2 and 9.3-15.9% inhibition of cell proliferation at 0.5 and 0.05 mg/mL, respectively. Phenolic extracts from whole barley kernel tested possessed high antioxidant, antiradical, and antiproliferative potentials. Therefore, inclusion of whole barley into the daily diet may render beneficial health benefits.

Authors+Show Affiliations

Department of Biochemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17542605

Citation

Madhujith, Terrence, and Fereidoon Shahidi. "Antioxidative and Antiproliferative Properties of Selected Barley (Hordeum Vulgarae L.) Cultivars and Their Potential for Inhibition of Low-density Lipoprotein (LDL) Cholesterol Oxidation." Journal of Agricultural and Food Chemistry, vol. 55, no. 13, 2007, pp. 5018-24.
Madhujith T, Shahidi F. Antioxidative and antiproliferative properties of selected barley (Hordeum vulgarae L.) cultivars and their potential for inhibition of low-density lipoprotein (LDL) cholesterol oxidation. J Agric Food Chem. 2007;55(13):5018-24.
Madhujith, T., & Shahidi, F. (2007). Antioxidative and antiproliferative properties of selected barley (Hordeum vulgarae L.) cultivars and their potential for inhibition of low-density lipoprotein (LDL) cholesterol oxidation. Journal of Agricultural and Food Chemistry, 55(13), 5018-24.
Madhujith T, Shahidi F. Antioxidative and Antiproliferative Properties of Selected Barley (Hordeum Vulgarae L.) Cultivars and Their Potential for Inhibition of Low-density Lipoprotein (LDL) Cholesterol Oxidation. J Agric Food Chem. 2007 Jun 27;55(13):5018-24. PubMed PMID: 17542605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative and antiproliferative properties of selected barley (Hordeum vulgarae L.) cultivars and their potential for inhibition of low-density lipoprotein (LDL) cholesterol oxidation. AU - Madhujith,Terrence, AU - Shahidi,Fereidoon, Y1 - 2007/06/02/ PY - 2007/6/5/pubmed PY - 2007/8/19/medline PY - 2007/6/5/entrez SP - 5018 EP - 24 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 55 IS - 13 N2 - Aqueous methanolic extracts of whole kernels from six different barley cultivars, namely, Falcon, AC Metcalfe, Tyto, Tercel, Phoenix, and Peregrine, were examined for their total phenolic content (TPC), oxygen radical scavenging capacity (ORACFL), hydroxyl radical scavenging capacity (HORACFL), potency in prevention of lipid oxidation using the Rancimat method, efficacy in inhibition of Cu(II)-induced human LDL cholesterol oxidation, and antiproliferative activities using Caco-2 colorectal adenocarcinoma cell line. Total phenolic content as measured by Folin-Ciocalteu's method ranged from 0.68 to 1.19 mg of ferulic acid equiv/g of defatted material, whereas ORACFL and HORACFL values were 11.28-19.10 and 9.06-12.99 micromol of Trolox equiv/g of defatted material, respectively. Protection factor (PF), a measure of the effect of extracts on the prevention of oxidation of stripped corn oil as measured by Rancimat, ranged from 0.97 to 1.59. Furthermore, barley extracts showed 19.64-33.93% inhibition against Cu(II)-induced human LDL cholesterol oxidation at a final concentration of 0.02 mg/mL. The proliferation of Caco-2 colon cancer cells was significantly (p < 0.05) inhibited in a dose-dependent fashion in the presence of all barley extracts tested at the end of the day 4 of incubation. At the end of day 4, barley extracts rendered 29.3-51.2 and 9.3-15.9% inhibition of cell proliferation at 0.5 and 0.05 mg/mL, respectively. Phenolic extracts from whole barley kernel tested possessed high antioxidant, antiradical, and antiproliferative potentials. Therefore, inclusion of whole barley into the daily diet may render beneficial health benefits. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/17542605/Antioxidative_and_antiproliferative_properties_of_selected_barley__Hordeum_vulgarae_L___cultivars_and_their_potential_for_inhibition_of_low_density_lipoprotein__LDL__cholesterol_oxidation_ L2 - https://doi.org/10.1021/jf070072a DB - PRIME DP - Unbound Medicine ER -