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Dose dependent effect of ricin on DNA damage and antioxidant enzymes in mice.
Cell Mol Biol (Noisy-le-grand). 2007 May 30; 53(5):92-102.CM

Abstract

Ricin a glycoprotein from the Ricinus communis seeds, is known to have diverse toxic effects on cells of different visceral organs. We have studied the effect of ricin (0.5, 1.0 and 2.0 LD50) on various oxidative stress markers at 1, 3 and 7 day post exposure following i.p. administration in Swiss albino male mice. Results of this study revealed that ricin induces generation of reactive species, lipidperoxidation, DNA fragmentation and depletion of GSH. Activity of antioxidant cascade related enzyme like superoxide dismutase (SOD), glutathione peroxidase (GPx) decreased, while glutathione reductase (GR) and catalase activity increased. Superoxide dismutase and glutathione peroxidase activity was decreased significantly in liver, spleen and kidney. The decrease was more prominent on 7 day of post exposure in all the exposed doses. A significant increase in the activities of catalase was observed in plasma, liver, spleen and kidney on 7 day following ricin exposure. Glutathione reductase increased significantly as early as 24 h following 1.0 LD50 dose. Lipid peroxidation increased and non protein sulfhydryl content decreased in all the tissues at different time intervals. Total antioxidant status was reduced as early as 1 day post exposure. Nearly two fold increase was observed in DNA fragmentation following 0.5 LD50 dose of ricin on 1 day post exposure. DNA diffusion assay also indicated an early damage to DNA due to ROS. An early change in DNA fragmentation, DNA diffusion, and total antioxidant status and in the activity of various enzymes indicates that ricin produce oxidative stress by generation of reactive oxygen species as early as 24 h at a minimum dose of 0.5 LD50. Probably this is the first study which indicate that ricin induced oxidative stress at a minimum dose of 0.5 LD50.

Authors+Show Affiliations

Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Gwalior, India. omkumar63@rediffmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17543238

Citation

Kumar, O, et al. "Dose Dependent Effect of Ricin On DNA Damage and Antioxidant Enzymes in Mice." Cellular and Molecular Biology (Noisy-le-Grand, France), vol. 53, no. 5, 2007, pp. 92-102.
Kumar O, Lakshmana Rao PV, Pradhan S, et al. Dose dependent effect of ricin on DNA damage and antioxidant enzymes in mice. Cell Mol Biol (Noisy-le-grand). 2007;53(5):92-102.
Kumar, O., Lakshmana Rao, P. V., Pradhan, S., Jayaraj, R., Bhaskar, A. S., Nashikkar, A. B., & Vijayaraghavan, R. (2007). Dose dependent effect of ricin on DNA damage and antioxidant enzymes in mice. Cellular and Molecular Biology (Noisy-le-Grand, France), 53(5), 92-102.
Kumar O, et al. Dose Dependent Effect of Ricin On DNA Damage and Antioxidant Enzymes in Mice. Cell Mol Biol (Noisy-le-grand). 2007 May 30;53(5):92-102. PubMed PMID: 17543238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dose dependent effect of ricin on DNA damage and antioxidant enzymes in mice. AU - Kumar,O, AU - Lakshmana Rao,P V, AU - Pradhan,S, AU - Jayaraj,R, AU - Bhaskar,A S B, AU - Nashikkar,A B, AU - Vijayaraghavan,R, Y1 - 2007/05/30/ PY - 2007/01/16/received PY - 2007/02/16/accepted PY - 2007/6/5/pubmed PY - 2007/7/12/medline PY - 2007/6/5/entrez SP - 92 EP - 102 JF - Cellular and molecular biology (Noisy-le-Grand, France) JO - Cell Mol Biol (Noisy-le-grand) VL - 53 IS - 5 N2 - Ricin a glycoprotein from the Ricinus communis seeds, is known to have diverse toxic effects on cells of different visceral organs. We have studied the effect of ricin (0.5, 1.0 and 2.0 LD50) on various oxidative stress markers at 1, 3 and 7 day post exposure following i.p. administration in Swiss albino male mice. Results of this study revealed that ricin induces generation of reactive species, lipidperoxidation, DNA fragmentation and depletion of GSH. Activity of antioxidant cascade related enzyme like superoxide dismutase (SOD), glutathione peroxidase (GPx) decreased, while glutathione reductase (GR) and catalase activity increased. Superoxide dismutase and glutathione peroxidase activity was decreased significantly in liver, spleen and kidney. The decrease was more prominent on 7 day of post exposure in all the exposed doses. A significant increase in the activities of catalase was observed in plasma, liver, spleen and kidney on 7 day following ricin exposure. Glutathione reductase increased significantly as early as 24 h following 1.0 LD50 dose. Lipid peroxidation increased and non protein sulfhydryl content decreased in all the tissues at different time intervals. Total antioxidant status was reduced as early as 1 day post exposure. Nearly two fold increase was observed in DNA fragmentation following 0.5 LD50 dose of ricin on 1 day post exposure. DNA diffusion assay also indicated an early damage to DNA due to ROS. An early change in DNA fragmentation, DNA diffusion, and total antioxidant status and in the activity of various enzymes indicates that ricin produce oxidative stress by generation of reactive oxygen species as early as 24 h at a minimum dose of 0.5 LD50. Probably this is the first study which indicate that ricin induced oxidative stress at a minimum dose of 0.5 LD50. SN - 1165-158X UR - https://www.unboundmedicine.com/medline/citation/17543238/Dose_dependent_effect_of_ricin_on_DNA_damage_and_antioxidant_enzymes_in_mice_ L2 - https://medlineplus.gov/antioxidants.html DB - PRIME DP - Unbound Medicine ER -