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Molecularly imprinted solid phase extraction for the selective HPLC determination of alpha-tocopherol in bay leaves.
Anal Chim Acta. 2007 Jun 19; 593(2):164-70.AC

Abstract

A new sorbent for molecularly imprinted solid phase extraction (MISPE) was synthesized to extract and purify alpha-tocopherol (alpha-TP) from vegetable sources. Molecularly imprinted polymers (MIP) were synthesized using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinking agent using a photo-polymerization procedure. A thermo-polymerization was also performed but no imprinting effect in the resulting materials was raised. The proposed MISPE protocol could overcome the drawback of traditional detection methods, which require pre-treatments of the samples. The possibility to obtain the selective recognition of alpha-TP from natural samples in aqueous mixtures represents one of the main advantages of our materials. Our procedure involves the direct HPLC injection of eluate without any treatment and above all the use of no toxic and biocompatible organic solvents. After the evaluation of the selectivity of the alpha-TP imprinted polymers, the performance of these materials as solid phase extraction (SPE) sorbents was investigated. Our MISPE-HPLC procedure has a high sensitivity, LOD and LOQ were 3.49x10(-7) and 1.16x10(-6) mol L(-1), respectively, as well as good precision (intraday precision below 3.3% and interday precisions below 6.5%) and recovery (60%). Thus, it can be successfully used for the purification of alpha-TP from bay leaves.

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Authors+Show Affiliations

Puoci F
Dipartimento di Scienze Farmaceutiche, Università della Calabria, Edificio Polifunzionale, Arcavacata di Rende (CS) 87036, Italy. francesco.puoci@unical.it
Cirillo G
No affiliation info available
Curcio M
No affiliation info available
Iemma F
No affiliation info available
Spizzirri UG
No affiliation info available
Picci N
No affiliation info available

MeSH

Chromatography, High Pressure LiquidLaurusPlant ExtractsPlant LeavesSolid Phase Extractionalpha-Tocopherol

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17543603

Citation

Puoci, F, et al. "Molecularly Imprinted Solid Phase Extraction for the Selective HPLC Determination of Alpha-tocopherol in Bay Leaves." Analytica Chimica Acta, vol. 593, no. 2, 2007, pp. 164-70.
Puoci F, Cirillo G, Curcio M, et al. Molecularly imprinted solid phase extraction for the selective HPLC determination of alpha-tocopherol in bay leaves. Anal Chim Acta. 2007;593(2):164-70.
Puoci, F., Cirillo, G., Curcio, M., Iemma, F., Spizzirri, U. G., & Picci, N. (2007). Molecularly imprinted solid phase extraction for the selective HPLC determination of alpha-tocopherol in bay leaves. Analytica Chimica Acta, 593(2), 164-70.
Puoci F, et al. Molecularly Imprinted Solid Phase Extraction for the Selective HPLC Determination of Alpha-tocopherol in Bay Leaves. Anal Chim Acta. 2007 Jun 19;593(2):164-70. PubMed PMID: 17543603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted solid phase extraction for the selective HPLC determination of alpha-tocopherol in bay leaves. AU - Puoci,F, AU - Cirillo,G, AU - Curcio,M, AU - Iemma,F, AU - Spizzirri,U G, AU - Picci,N, Y1 - 2007/05/01/ PY - 2007/03/02/received PY - 2007/04/24/revised PY - 2007/04/25/accepted PY - 2007/6/5/pubmed PY - 2007/8/9/medline PY - 2007/6/5/entrez SP - 164 EP - 70 JF - Analytica chimica acta JO - Anal Chim Acta VL - 593 IS - 2 N2 - A new sorbent for molecularly imprinted solid phase extraction (MISPE) was synthesized to extract and purify alpha-tocopherol (alpha-TP) from vegetable sources. Molecularly imprinted polymers (MIP) were synthesized using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as crosslinking agent using a photo-polymerization procedure. A thermo-polymerization was also performed but no imprinting effect in the resulting materials was raised. The proposed MISPE protocol could overcome the drawback of traditional detection methods, which require pre-treatments of the samples. The possibility to obtain the selective recognition of alpha-TP from natural samples in aqueous mixtures represents one of the main advantages of our materials. Our procedure involves the direct HPLC injection of eluate without any treatment and above all the use of no toxic and biocompatible organic solvents. After the evaluation of the selectivity of the alpha-TP imprinted polymers, the performance of these materials as solid phase extraction (SPE) sorbents was investigated. Our MISPE-HPLC procedure has a high sensitivity, LOD and LOQ were 3.49x10(-7) and 1.16x10(-6) mol L(-1), respectively, as well as good precision (intraday precision below 3.3% and interday precisions below 6.5%) and recovery (60%). Thus, it can be successfully used for the purification of alpha-TP from bay leaves. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/17543603/Molecularly_imprinted_solid_phase_extraction_for_the_selective_HPLC_determination_of_alpha_tocopherol_in_bay_leaves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(07)00837-9 DB - PRIME DP - Unbound Medicine ER -
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