Tags

Type your tag names separated by a space and hit enter

Chronic intrathecal infusion of minocycline prevents the development of spinal-nerve ligation-induced pain in rats.
Reg Anesth Pain Med. 2007 May-Jun; 32(3):209-16.RA

Abstract

BACKGROUND AND OBJECTIVES

Minocycline is a second-generation tetracycline with multiple biological effects, including inhibition of microglial activation. Recently, microglial activation has been implicated in the development of nerve injury-induced neuropathic pain. In this study, the authors examined the effects of continuous intrathecal minocycline on the development of neuropathic pain and microglial activation induced by L5/6 spinal-nerve ligation in rats.

METHODS

Under isoflurane anesthesia, male Sprague-Dawley rats (200-250 g) received right L5/6 spinal-nerve ligation and intrathecal catheters connected to an infusion pump. Intrathecal saline or minocycline (2 and 6 microg/h) was given continuously after surgery for 7 days (n = 8 per group). The rat right hind paw withdrawal threshold to von Frey filament stimuli and withdrawal latency to radiant heat were determined before surgery and on days 1 to 7 after surgery. Spinal microglial activation was evaluated with OX-42 immunoreactivity on day 7 after surgery.

RESULTS

Spinal-nerve ligation induced mechanical allodynia and thermal hyperalgesia on the affected hind paw of saline-treated rats. Intrathecal minocycline (2 and 6 microg/h) prevented the development of mechanical allodynia and thermal hyperalgesia induced by nerve ligation. It also inhibited nerve ligation-induced microglial activation, as evidenced by decreased OX-42 staining. No obvious histopathologic change was noted after intrathecal minocycline (6 microg/h) infusion.

CONCLUSIONS

In this study, the authors demonstrate the preventive effect of continuous intrathecal minocycline on the development of nociceptive behaviors induced by L5/6 spinal-nerve ligation in rats. Further studies are required to examine if continuous intrathecal minocycline could be used safely in the clinical setting.

Authors+Show Affiliations

Department of Anesthesiology, Mackay Memorial Hospital, Taipei, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17543815

Citation

Lin, Chia-Shiang, et al. "Chronic Intrathecal Infusion of Minocycline Prevents the Development of Spinal-nerve Ligation-induced Pain in Rats." Regional Anesthesia and Pain Medicine, vol. 32, no. 3, 2007, pp. 209-16.
Lin CS, Tsaur ML, Chen CC, et al. Chronic intrathecal infusion of minocycline prevents the development of spinal-nerve ligation-induced pain in rats. Reg Anesth Pain Med. 2007;32(3):209-16.
Lin, C. S., Tsaur, M. L., Chen, C. C., Wang, T. Y., Lin, C. F., Lai, Y. L., Hsu, T. C., Pan, Y. Y., Yang, C. H., & Cheng, J. K. (2007). Chronic intrathecal infusion of minocycline prevents the development of spinal-nerve ligation-induced pain in rats. Regional Anesthesia and Pain Medicine, 32(3), 209-16.
Lin CS, et al. Chronic Intrathecal Infusion of Minocycline Prevents the Development of Spinal-nerve Ligation-induced Pain in Rats. Reg Anesth Pain Med. 2007 May-Jun;32(3):209-16. PubMed PMID: 17543815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chronic intrathecal infusion of minocycline prevents the development of spinal-nerve ligation-induced pain in rats. AU - Lin,Chia-Shiang, AU - Tsaur,Meei-Ling, AU - Chen,Chien-Chuan, AU - Wang,Tao-Yeuan, AU - Lin,Chih-Fu, AU - Lai,Yuen-Liang, AU - Hsu,Tien-Chi, AU - Pan,Yu-Yen, AU - Yang,Chen-Hsien, AU - Cheng,Jen-Kun, PY - 2006/07/09/received PY - 2007/01/22/revised PY - 2007/01/27/accepted PY - 2007/6/5/pubmed PY - 2007/8/2/medline PY - 2007/6/5/entrez SP - 209 EP - 16 JF - Regional anesthesia and pain medicine JO - Reg Anesth Pain Med VL - 32 IS - 3 N2 - BACKGROUND AND OBJECTIVES: Minocycline is a second-generation tetracycline with multiple biological effects, including inhibition of microglial activation. Recently, microglial activation has been implicated in the development of nerve injury-induced neuropathic pain. In this study, the authors examined the effects of continuous intrathecal minocycline on the development of neuropathic pain and microglial activation induced by L5/6 spinal-nerve ligation in rats. METHODS: Under isoflurane anesthesia, male Sprague-Dawley rats (200-250 g) received right L5/6 spinal-nerve ligation and intrathecal catheters connected to an infusion pump. Intrathecal saline or minocycline (2 and 6 microg/h) was given continuously after surgery for 7 days (n = 8 per group). The rat right hind paw withdrawal threshold to von Frey filament stimuli and withdrawal latency to radiant heat were determined before surgery and on days 1 to 7 after surgery. Spinal microglial activation was evaluated with OX-42 immunoreactivity on day 7 after surgery. RESULTS: Spinal-nerve ligation induced mechanical allodynia and thermal hyperalgesia on the affected hind paw of saline-treated rats. Intrathecal minocycline (2 and 6 microg/h) prevented the development of mechanical allodynia and thermal hyperalgesia induced by nerve ligation. It also inhibited nerve ligation-induced microglial activation, as evidenced by decreased OX-42 staining. No obvious histopathologic change was noted after intrathecal minocycline (6 microg/h) infusion. CONCLUSIONS: In this study, the authors demonstrate the preventive effect of continuous intrathecal minocycline on the development of nociceptive behaviors induced by L5/6 spinal-nerve ligation in rats. Further studies are required to examine if continuous intrathecal minocycline could be used safely in the clinical setting. SN - 1098-7339 UR - https://www.unboundmedicine.com/medline/citation/17543815/Chronic_intrathecal_infusion_of_minocycline_prevents_the_development_of_spinal_nerve_ligation_induced_pain_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1098-7339(07)00061-2 DB - PRIME DP - Unbound Medicine ER -