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Effect of nutrient starvation on the cellular composition and metabolic capacity of Saccharomyces cerevisiae.
Appl Environ Microbiol. 2007 Aug; 73(15):4839-48.AE

Abstract

This investigation addresses the following question: what are the important factors for maintenance of a high catabolic capacity under various starvation conditions? Saccharomyces cerevisiae was cultured in aerobic batch cultures, and during the diauxic shift cells were transferred and subjected to 24 h of starvation. The following conditions were used: carbon starvation, nitrogen starvation in the presence of glucose or ethanol, and both carbon starvation and nitrogen starvation. During the starvation period changes in biomass composition (including protein, carbohydrate, lipid, and nucleic acid contents), metabolic activity, sugar transport kinetics, and the levels of selected enzymes were recorded. Subsequent to the starvation period the remaining catabolic capacity was measured by addition of 50 mM glucose. The results showed that the glucose transport capacity is a key factor for maintenance of high metabolic capacity in many, but not all, cases. The results for cells starved of carbon, carbon and nitrogen, or nitrogen in the presence of glucose all indicated that the metabolic capacity was indeed controlled by the glucose transport ability, perhaps with some influence of hexokinase, phosphofructokinase, aldolase, and enolase levels. However, it was also demonstrated that there was no such correlation when nitrogen starvation occurred in the presence of ethanol instead of glucose.

Authors+Show Affiliations

Department of Chemical and Biological Engineering-Molecular Biotechnology, Chalmers University of Technology, Box 462, SE-405 30 Göteborg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17545328

Citation

Albers, Eva, et al. "Effect of Nutrient Starvation On the Cellular Composition and Metabolic Capacity of Saccharomyces Cerevisiae." Applied and Environmental Microbiology, vol. 73, no. 15, 2007, pp. 4839-48.
Albers E, Larsson C, Andlid T, et al. Effect of nutrient starvation on the cellular composition and metabolic capacity of Saccharomyces cerevisiae. Appl Environ Microbiol. 2007;73(15):4839-48.
Albers, E., Larsson, C., Andlid, T., Walsh, M. C., & Gustafsson, L. (2007). Effect of nutrient starvation on the cellular composition and metabolic capacity of Saccharomyces cerevisiae. Applied and Environmental Microbiology, 73(15), 4839-48.
Albers E, et al. Effect of Nutrient Starvation On the Cellular Composition and Metabolic Capacity of Saccharomyces Cerevisiae. Appl Environ Microbiol. 2007;73(15):4839-48. PubMed PMID: 17545328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of nutrient starvation on the cellular composition and metabolic capacity of Saccharomyces cerevisiae. AU - Albers,Eva, AU - Larsson,Christer, AU - Andlid,Thomas, AU - Walsh,Michael C, AU - Gustafsson,Lena, Y1 - 2007/06/01/ PY - 2007/6/5/pubmed PY - 2007/10/11/medline PY - 2007/6/5/entrez SP - 4839 EP - 48 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 73 IS - 15 N2 - This investigation addresses the following question: what are the important factors for maintenance of a high catabolic capacity under various starvation conditions? Saccharomyces cerevisiae was cultured in aerobic batch cultures, and during the diauxic shift cells were transferred and subjected to 24 h of starvation. The following conditions were used: carbon starvation, nitrogen starvation in the presence of glucose or ethanol, and both carbon starvation and nitrogen starvation. During the starvation period changes in biomass composition (including protein, carbohydrate, lipid, and nucleic acid contents), metabolic activity, sugar transport kinetics, and the levels of selected enzymes were recorded. Subsequent to the starvation period the remaining catabolic capacity was measured by addition of 50 mM glucose. The results showed that the glucose transport capacity is a key factor for maintenance of high metabolic capacity in many, but not all, cases. The results for cells starved of carbon, carbon and nitrogen, or nitrogen in the presence of glucose all indicated that the metabolic capacity was indeed controlled by the glucose transport ability, perhaps with some influence of hexokinase, phosphofructokinase, aldolase, and enolase levels. However, it was also demonstrated that there was no such correlation when nitrogen starvation occurred in the presence of ethanol instead of glucose. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/17545328/Effect_of_nutrient_starvation_on_the_cellular_composition_and_metabolic_capacity_of_Saccharomyces_cerevisiae_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=17545328 DB - PRIME DP - Unbound Medicine ER -