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Development and molecular cytogenetic identification of new winter wheat--winter barley ('Martonvásári 9 kr1' - 'Igri') disomic addition lines.
Genome. 2007 Jan; 50(1):43-50.G

Abstract

This paper describes a series of winter wheat - winter barley disomic addition lines developed from hybrids between winter wheat line Triticum aestivum L. 'Martonvásári 9 kr1' and the German 2-rowed winter barley cultivar Hordeum vulgare L. 'Igri'. The barley chromosomes in a wheat background were identified from the fluorescent in situ hybridization (FISH) patterns obtained with various combinations of repetitive DNA probes: GAA-HvT01 and pTa71-HvT01. The disomic addition lines 2H, 3H, and 4H and the 1HS isochromosome were identified on the basis of a 2-colour FISH with the DNA probe pairs GAA-pAs1, GAA-HvT01, and pTa71-HvT01. Genomic in situ hybridization was used to confirm the presence of the barley chromosomes in the wheat genome. The identification of the barley chromosomes in the addition lines was further confirmed with simple-sequence repeat markers. The addition lines were also characterized morphologically.

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Authors+Show Affiliations

Szakács E
Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462 Martonvásár, P.O. Box 19, Hungary.
Molnár-Láng M
No affiliation info available

MeSH

Chromosome BandingChromosome MappingChromosomes, PlantCold ClimateCrosses, GeneticGenes, PlantGermanyHordeumHungaryKaryotypingSeasonsTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17546070

Citation

Szakács, E, and M Molnár-Láng. "Development and Molecular Cytogenetic Identification of New Winter Wheat--winter Barley ('Martonvásári 9 Kr1' - 'Igri') Disomic Addition Lines." Genome, vol. 50, no. 1, 2007, pp. 43-50.
Szakács E, Molnár-Láng M. Development and molecular cytogenetic identification of new winter wheat--winter barley ('Martonvásári 9 kr1' - 'Igri') disomic addition lines. Genome. 2007;50(1):43-50.
Szakács, E., & Molnár-Láng, M. (2007). Development and molecular cytogenetic identification of new winter wheat--winter barley ('Martonvásári 9 kr1' - 'Igri') disomic addition lines. Genome, 50(1), 43-50.
Szakács E, Molnár-Láng M. Development and Molecular Cytogenetic Identification of New Winter Wheat--winter Barley ('Martonvásári 9 Kr1' - 'Igri') Disomic Addition Lines. Genome. 2007;50(1):43-50. PubMed PMID: 17546070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and molecular cytogenetic identification of new winter wheat--winter barley ('Martonvásári 9 kr1' - 'Igri') disomic addition lines. AU - Szakács,E, AU - Molnár-Láng,M, PY - 2007/6/5/pubmed PY - 2007/8/7/medline PY - 2007/6/5/entrez SP - 43 EP - 50 JF - Genome JO - Genome VL - 50 IS - 1 N2 - This paper describes a series of winter wheat - winter barley disomic addition lines developed from hybrids between winter wheat line Triticum aestivum L. 'Martonvásári 9 kr1' and the German 2-rowed winter barley cultivar Hordeum vulgare L. 'Igri'. The barley chromosomes in a wheat background were identified from the fluorescent in situ hybridization (FISH) patterns obtained with various combinations of repetitive DNA probes: GAA-HvT01 and pTa71-HvT01. The disomic addition lines 2H, 3H, and 4H and the 1HS isochromosome were identified on the basis of a 2-colour FISH with the DNA probe pairs GAA-pAs1, GAA-HvT01, and pTa71-HvT01. Genomic in situ hybridization was used to confirm the presence of the barley chromosomes in the wheat genome. The identification of the barley chromosomes in the addition lines was further confirmed with simple-sequence repeat markers. The addition lines were also characterized morphologically. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/17546070/Development_and_molecular_cytogenetic_identification_of_new_winter_wheat__winter_barley__'Martonvásári_9_kr1'___'Igri'__disomic_addition_lines_ L2 - https://cdnsciencepub.com/doi/10.1139/g06-134?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -