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Determination of three natural pesticides in processed fruit and vegetables using high-performance liquid chromatography/tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2007; 21(13):2079-86.RC

Abstract

This paper describes a method for the sensitive and selective determination of two macrocyclic lactones (abamectin and spinosad) and azadirachtin in apple purée, concentrated lemon juice, tomato purée and canned peas. The general sample extraction-partitioning method for our gas chromatography and liquid chromatography multiresidue methods has been used. The analytical procedure involves an extraction with acetone and liquid-liquid partitioning with ethyl acetate/cyclohexane combined in one step. The extracts are analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) without any further clean-up step. The pesticides are separated on a reversed-phase C12 column using a gradient elution. Thirteen simultaneous MS/MS transitions of precursor ions were monitored. Studies at fortification levels of 2.5-10 microg/kg and 25-100 microg/kg gave mean recoveries ranging from 70-100% for all compounds with satisfactory precision (relative standard deviation (RSD) from 3-20%). The excellent selectivity and sensitivity allows quantification and identification of low levels of pesticides in canned peas, tomato and apple purées (limits of quantitation (LOQs) 1-5 microg/kg) and in concentrated lemon juice (LOQs 2-10 microg/kg). The quantification of analytes was carried out using the most sensitive transition for every compound and by 'matrix-matched' standards calibration.

Authors+Show Affiliations

Stazione Sperimentale per l'Industria delle Conserve Alimentari, Viale F.Tanara 31/A 43100 Parma, Italy. anna.sannino@ssica.it

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17546651

Citation

Sannino, Anna. "Determination of Three Natural Pesticides in Processed Fruit and Vegetables Using High-performance Liquid Chromatography/tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 13, 2007, pp. 2079-86.
Sannino A. Determination of three natural pesticides in processed fruit and vegetables using high-performance liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2007;21(13):2079-86.
Sannino, A. (2007). Determination of three natural pesticides in processed fruit and vegetables using high-performance liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 21(13), 2079-86.
Sannino A. Determination of Three Natural Pesticides in Processed Fruit and Vegetables Using High-performance Liquid Chromatography/tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2007;21(13):2079-86. PubMed PMID: 17546651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of three natural pesticides in processed fruit and vegetables using high-performance liquid chromatography/tandem mass spectrometry. A1 - Sannino,Anna, PY - 2007/6/5/pubmed PY - 2007/8/3/medline PY - 2007/6/5/entrez SP - 2079 EP - 86 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 13 N2 - This paper describes a method for the sensitive and selective determination of two macrocyclic lactones (abamectin and spinosad) and azadirachtin in apple purée, concentrated lemon juice, tomato purée and canned peas. The general sample extraction-partitioning method for our gas chromatography and liquid chromatography multiresidue methods has been used. The analytical procedure involves an extraction with acetone and liquid-liquid partitioning with ethyl acetate/cyclohexane combined in one step. The extracts are analyzed by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) without any further clean-up step. The pesticides are separated on a reversed-phase C12 column using a gradient elution. Thirteen simultaneous MS/MS transitions of precursor ions were monitored. Studies at fortification levels of 2.5-10 microg/kg and 25-100 microg/kg gave mean recoveries ranging from 70-100% for all compounds with satisfactory precision (relative standard deviation (RSD) from 3-20%). The excellent selectivity and sensitivity allows quantification and identification of low levels of pesticides in canned peas, tomato and apple purées (limits of quantitation (LOQs) 1-5 microg/kg) and in concentrated lemon juice (LOQs 2-10 microg/kg). The quantification of analytes was carried out using the most sensitive transition for every compound and by 'matrix-matched' standards calibration. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17546651/Determination_of_three_natural_pesticides_in_processed_fruit_and_vegetables_using_high_performance_liquid_chromatography/tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.3051 DB - PRIME DP - Unbound Medicine ER -