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N-acetylcysteine inhibits activation of toll-like receptor 2 and 4 gene expression in the liver and lung after partial hepatic ischemia-reperfusion injury in mice.
Hepatobiliary Pancreat Dis Int. 2007 Jun; 6(3):284-9.HP

Abstract

BACKGROUND

Toll-like receptor 2 and 4 (TLR2/4) may play important roles in ischemia-reperfusion (I/R) injury, and N-acetylcysteine (NAC) can prevent the generation of reactive oxygen species (ROS) induced by I/R injury. This study aimed to investigate the changes in TLR2/4 gene expression in the liver and lung after I/R injury with or without NAC pretreatment.

METHODS

BALB/c mice were used in a model of partial hepatic I/R injury and randomly assigned to a sham-operated control group (SH), a hepatic ischemia/reperfusion group (I/R) or a NAC pretreated, hepatic I/R group (I/R-NAC). The levels of TNF-alpha in the portal vein and plasma alanine aminotransferase (ALT) were measured at 1 and 3 hours after reperfusion. The lung wet-to-dry ratio was measured, and the expression of TLR2/4 mRNA and protein in the liver and lung were assessed with RT-PCR and Western blotting at the same time points.

RESULTS

Compared with the I/R group, the expression of TLR2/4 mRNA and protein in the liver and lung in the I/R-NAC group was decreased at the same time point (P<0.05). The levels of portal vein TNF-alpha and plasma ALT increased continuously in the I/R group at 1 and 3 hours of reperfusion compared with the SH group; however, they declined significantly in the group pretreated with NAC (P<0.05). The extent of lung edema was relieved in the I/R-NAC group compared with the I/R group (P<0.05).

CONCLUSIONS

TLR2/4 was activated in the liver and lung in the process of partial hepatic I/R injury. NAC inhibited the activation of TLR2/4 and the induction of TNF-alpha resulting from I/R injury via modulating the redox state, thus it may mitigate liver and lung injury following partial hepatic I/R in mice.

Authors+Show Affiliations

Center of Pancreatic Surgery, Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17548252

Citation

Jin, Xin, et al. "N-acetylcysteine Inhibits Activation of Toll-like Receptor 2 and 4 Gene Expression in the Liver and Lung After Partial Hepatic Ischemia-reperfusion Injury in Mice." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 6, no. 3, 2007, pp. 284-9.
Jin X, Wang L, Wu HS, et al. N-acetylcysteine inhibits activation of toll-like receptor 2 and 4 gene expression in the liver and lung after partial hepatic ischemia-reperfusion injury in mice. Hepatobiliary Pancreat Dis Int. 2007;6(3):284-9.
Jin, X., Wang, L., Wu, H. S., Zhang, L., Wang, C. Y., Tian, Y., & Zhang, J. H. (2007). N-acetylcysteine inhibits activation of toll-like receptor 2 and 4 gene expression in the liver and lung after partial hepatic ischemia-reperfusion injury in mice. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 6(3), 284-9.
Jin X, et al. N-acetylcysteine Inhibits Activation of Toll-like Receptor 2 and 4 Gene Expression in the Liver and Lung After Partial Hepatic Ischemia-reperfusion Injury in Mice. Hepatobiliary Pancreat Dis Int. 2007;6(3):284-9. PubMed PMID: 17548252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-acetylcysteine inhibits activation of toll-like receptor 2 and 4 gene expression in the liver and lung after partial hepatic ischemia-reperfusion injury in mice. AU - Jin,Xin, AU - Wang,Lin, AU - Wu,He-Shui, AU - Zhang,Lei, AU - Wang,Chun-You, AU - Tian,Yuan, AU - Zhang,Jing-Hui, PY - 2007/6/6/pubmed PY - 2007/7/20/medline PY - 2007/6/6/entrez SP - 284 EP - 9 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - Hepatobiliary Pancreat Dis Int VL - 6 IS - 3 N2 - BACKGROUND: Toll-like receptor 2 and 4 (TLR2/4) may play important roles in ischemia-reperfusion (I/R) injury, and N-acetylcysteine (NAC) can prevent the generation of reactive oxygen species (ROS) induced by I/R injury. This study aimed to investigate the changes in TLR2/4 gene expression in the liver and lung after I/R injury with or without NAC pretreatment. METHODS: BALB/c mice were used in a model of partial hepatic I/R injury and randomly assigned to a sham-operated control group (SH), a hepatic ischemia/reperfusion group (I/R) or a NAC pretreated, hepatic I/R group (I/R-NAC). The levels of TNF-alpha in the portal vein and plasma alanine aminotransferase (ALT) were measured at 1 and 3 hours after reperfusion. The lung wet-to-dry ratio was measured, and the expression of TLR2/4 mRNA and protein in the liver and lung were assessed with RT-PCR and Western blotting at the same time points. RESULTS: Compared with the I/R group, the expression of TLR2/4 mRNA and protein in the liver and lung in the I/R-NAC group was decreased at the same time point (P<0.05). The levels of portal vein TNF-alpha and plasma ALT increased continuously in the I/R group at 1 and 3 hours of reperfusion compared with the SH group; however, they declined significantly in the group pretreated with NAC (P<0.05). The extent of lung edema was relieved in the I/R-NAC group compared with the I/R group (P<0.05). CONCLUSIONS: TLR2/4 was activated in the liver and lung in the process of partial hepatic I/R injury. NAC inhibited the activation of TLR2/4 and the induction of TNF-alpha resulting from I/R injury via modulating the redox state, thus it may mitigate liver and lung injury following partial hepatic I/R in mice. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/17548252/N_acetylcysteine_inhibits_activation_of_toll_like_receptor_2_and_4_gene_expression_in_the_liver_and_lung_after_partial_hepatic_ischemia_reperfusion_injury_in_mice_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:17548252 DB - PRIME DP - Unbound Medicine ER -