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Diagnosis of pancreatic cancer by cytology and telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopy.
Hepatobiliary Pancreat Dis Int. 2007 Jun; 6(3):308-11.HP

Abstract

BACKGROUND

Telomerase activity is reported to be specific and frequent in human pancreatic cancer. We conducted this study to assess the usefulness of monitoring telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) for the diagnosis of pancreatic cancer.

METHODS

Exfoliated cells obtained by pancreatic duct brushing during ERCP from 21 patients (18 with pancreatic cancer, 3 with chronic pancreatitis) were examined. Telomerase activity was detected by polymerase chain reaction and telomeric repeat amplification protocol assay (PCR-TRAP-ELISA).

RESULTS

D450 values of telomerase activity were 0.446+/-0.2700 in pancreatic cancer and 0.041+/-0.0111 in chronic pancreatitis. 77.8% (14/18) of patients with pancreatic cancer had cells with telomerase activity. None of the samples from patients with chronic pancreatitis showed telomerase activity, when the cutoff value of telomerase activity was set at 2.0. Cytological examination showed cancer cells in 66.7% (12/18) of the patients.

CONCLUSIONS

Telomerase activity may be an early malignant event in pancreatic cancer development. Cytology and telomerase activity in cells obtained by pancreatic duct brushing may complement each other for the diagnosis of pancreatic cancer.

Authors+Show Affiliations

Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China. zhouguoxiong@pub.nt.jsinfo.netNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17548257

Citation

Zhou, Guo-Xiong, et al. "Diagnosis of Pancreatic Cancer By Cytology and Telomerase Activity in Exfoliated Cells Obtained By Pancreatic Duct Brushing During Endoscopy." Hepatobiliary & Pancreatic Diseases International : HBPD INT, vol. 6, no. 3, 2007, pp. 308-11.
Zhou GX, Huang JF, Zhang H, et al. Diagnosis of pancreatic cancer by cytology and telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopy. HBPD INT. 2007;6(3):308-11.
Zhou, G. X., Huang, J. F., Zhang, H., & Chen, J. P. (2007). Diagnosis of pancreatic cancer by cytology and telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopy. Hepatobiliary & Pancreatic Diseases International : HBPD INT, 6(3), 308-11.
Zhou GX, et al. Diagnosis of Pancreatic Cancer By Cytology and Telomerase Activity in Exfoliated Cells Obtained By Pancreatic Duct Brushing During Endoscopy. HBPD INT. 2007;6(3):308-11. PubMed PMID: 17548257.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of pancreatic cancer by cytology and telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopy. AU - Zhou,Guo-Xiong, AU - Huang,Jie-Fei, AU - Zhang,Hong, AU - Chen,Jian-Ping, PY - 2007/6/6/pubmed PY - 2007/7/20/medline PY - 2007/6/6/entrez SP - 308 EP - 11 JF - Hepatobiliary & pancreatic diseases international : HBPD INT JO - HBPD INT VL - 6 IS - 3 N2 - BACKGROUND: Telomerase activity is reported to be specific and frequent in human pancreatic cancer. We conducted this study to assess the usefulness of monitoring telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) for the diagnosis of pancreatic cancer. METHODS: Exfoliated cells obtained by pancreatic duct brushing during ERCP from 21 patients (18 with pancreatic cancer, 3 with chronic pancreatitis) were examined. Telomerase activity was detected by polymerase chain reaction and telomeric repeat amplification protocol assay (PCR-TRAP-ELISA). RESULTS: D450 values of telomerase activity were 0.446+/-0.2700 in pancreatic cancer and 0.041+/-0.0111 in chronic pancreatitis. 77.8% (14/18) of patients with pancreatic cancer had cells with telomerase activity. None of the samples from patients with chronic pancreatitis showed telomerase activity, when the cutoff value of telomerase activity was set at 2.0. Cytological examination showed cancer cells in 66.7% (12/18) of the patients. CONCLUSIONS: Telomerase activity may be an early malignant event in pancreatic cancer development. Cytology and telomerase activity in cells obtained by pancreatic duct brushing may complement each other for the diagnosis of pancreatic cancer. SN - 1499-3872 UR - https://www.unboundmedicine.com/medline/citation/17548257/Diagnosis_of_pancreatic_cancer_by_cytology_and_telomerase_activity_in_exfoliated_cells_obtained_by_pancreatic_duct_brushing_during_endoscopy_ L2 - http://www.diseaseinfosearch.org/result/5551 DB - PRIME DP - Unbound Medicine ER -