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Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determination.
J Cell Biol. 2007 Jun 04; 177(5):769-79.JC

Abstract

Postnatal growth and regeneration of skeletal muscle requires a population of resident myogenic precursors named satellite cells. The transcription factor Pax7 is critical for satellite cell biogenesis and survival and has been also implicated in satellite cell self-renewal; however, the underlying molecular mechanisms remain unclear. Previously, we showed that Pax7 overexpression in adult primary myoblasts down-regulates MyoD and prevents myogenin induction, inhibiting myogenesis. We show that Pax7 prevents muscle differentiation independently of its transcriptional activity, affecting MyoD function. Conversely, myogenin directly affects Pax7 expression and may be critical for Pax7 down-regulation in differentiating cells. Our results provide evidence for a cross-inhibitory interaction between Pax7 and members of the muscle regulatory factor family. This could represent an additional mechanism for the control of satellite cell fate decisions resulting in proliferation, differentiation, and self-renewal, necessary for skeletal muscle maintenance and repair.

Authors+Show Affiliations

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17548510

Citation

Olguin, Hugo C., et al. "Reciprocal Inhibition Between Pax7 and Muscle Regulatory Factors Modulates Myogenic Cell Fate Determination." The Journal of Cell Biology, vol. 177, no. 5, 2007, pp. 769-79.
Olguin HC, Yang Z, Tapscott SJ, et al. Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determination. J Cell Biol. 2007;177(5):769-79.
Olguin, H. C., Yang, Z., Tapscott, S. J., & Olwin, B. B. (2007). Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determination. The Journal of Cell Biology, 177(5), 769-79.
Olguin HC, et al. Reciprocal Inhibition Between Pax7 and Muscle Regulatory Factors Modulates Myogenic Cell Fate Determination. J Cell Biol. 2007 Jun 4;177(5):769-79. PubMed PMID: 17548510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determination. AU - Olguin,Hugo C, AU - Yang,Zhihong, AU - Tapscott,Stephen J, AU - Olwin,Bradley B, PY - 2007/6/6/pubmed PY - 2007/8/7/medline PY - 2007/6/6/entrez SP - 769 EP - 79 JF - The Journal of cell biology JO - J Cell Biol VL - 177 IS - 5 N2 - Postnatal growth and regeneration of skeletal muscle requires a population of resident myogenic precursors named satellite cells. The transcription factor Pax7 is critical for satellite cell biogenesis and survival and has been also implicated in satellite cell self-renewal; however, the underlying molecular mechanisms remain unclear. Previously, we showed that Pax7 overexpression in adult primary myoblasts down-regulates MyoD and prevents myogenin induction, inhibiting myogenesis. We show that Pax7 prevents muscle differentiation independently of its transcriptional activity, affecting MyoD function. Conversely, myogenin directly affects Pax7 expression and may be critical for Pax7 down-regulation in differentiating cells. Our results provide evidence for a cross-inhibitory interaction between Pax7 and members of the muscle regulatory factor family. This could represent an additional mechanism for the control of satellite cell fate decisions resulting in proliferation, differentiation, and self-renewal, necessary for skeletal muscle maintenance and repair. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/17548510/Reciprocal_inhibition_between_Pax7_and_muscle_regulatory_factors_modulates_myogenic_cell_fate_determination_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200608122 DB - PRIME DP - Unbound Medicine ER -