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Tissue and life-stage distribution of a defensin gene in the Lone Star tick, Amblyomma americanum.
Med Vet Entomol. 2007 Jun; 21(2):141-7.MV

Abstract

The transcript sequence of the Amblyomma americanum Linnaeus (Acari: Ixodidae) defensin, termed amercin (amn), was ascertained and a 219-bp amn coding region identified. The gene encodes a 72-amino acid prepropeptide with a putative 37-amino acid mature peptide. This gene shows little similarity to either of the defensins from Amblyomma hebraeum Koch, the only other Amblyomma species for which a defensin has been described. Sequence comparisons with other tick defensins reveal amn to be shorter (6 bp or 2 amino acids) than the Ixodes scapularis Linnaeus (Acari: Ixodidae) and Dermacentor variabilis (Say) (Acari: Ixodidae) defensin sequences. The amercin prepropeptide has 60.8% and 59.5% similarity with the I. scapularis and D. variabilis prepropeptides, respectively, whereas the mature amercin peptide has 73.7% and 71.1% similarity with the mature peptides of these ticks. Similarity with other tick defensins ranges from 42% to 71%. In A. americanum, defensin transcript was found in the midgut, fat body and salivary gland tissues, as well as in the haemocytes. Defensin transcript was also present in early-stage eggs (less than 48 h old), late-stage eggs (approximately 2 weeks old), larvae and nymphs of A. americanum and I. scapularis, both of which are vector-competent for Borrelia spirochetes.

Authors+Show Affiliations

Department of Biological Sciences, Old Dominion University, Norfolk, Virginia 23529, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17550433

Citation

Todd, S M., et al. "Tissue and Life-stage Distribution of a Defensin Gene in the Lone Star Tick, Amblyomma Americanum." Medical and Veterinary Entomology, vol. 21, no. 2, 2007, pp. 141-7.
Todd SM, Sonenshine DE, Hynes WL. Tissue and life-stage distribution of a defensin gene in the Lone Star tick, Amblyomma americanum. Med Vet Entomol. 2007;21(2):141-7.
Todd, S. M., Sonenshine, D. E., & Hynes, W. L. (2007). Tissue and life-stage distribution of a defensin gene in the Lone Star tick, Amblyomma americanum. Medical and Veterinary Entomology, 21(2), 141-7.
Todd SM, Sonenshine DE, Hynes WL. Tissue and Life-stage Distribution of a Defensin Gene in the Lone Star Tick, Amblyomma Americanum. Med Vet Entomol. 2007;21(2):141-7. PubMed PMID: 17550433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue and life-stage distribution of a defensin gene in the Lone Star tick, Amblyomma americanum. AU - Todd,S M, AU - Sonenshine,D E, AU - Hynes,W L, PY - 2007/6/7/pubmed PY - 2007/10/24/medline PY - 2007/6/7/entrez SP - 141 EP - 7 JF - Medical and veterinary entomology JO - Med. Vet. Entomol. VL - 21 IS - 2 N2 - The transcript sequence of the Amblyomma americanum Linnaeus (Acari: Ixodidae) defensin, termed amercin (amn), was ascertained and a 219-bp amn coding region identified. The gene encodes a 72-amino acid prepropeptide with a putative 37-amino acid mature peptide. This gene shows little similarity to either of the defensins from Amblyomma hebraeum Koch, the only other Amblyomma species for which a defensin has been described. Sequence comparisons with other tick defensins reveal amn to be shorter (6 bp or 2 amino acids) than the Ixodes scapularis Linnaeus (Acari: Ixodidae) and Dermacentor variabilis (Say) (Acari: Ixodidae) defensin sequences. The amercin prepropeptide has 60.8% and 59.5% similarity with the I. scapularis and D. variabilis prepropeptides, respectively, whereas the mature amercin peptide has 73.7% and 71.1% similarity with the mature peptides of these ticks. Similarity with other tick defensins ranges from 42% to 71%. In A. americanum, defensin transcript was found in the midgut, fat body and salivary gland tissues, as well as in the haemocytes. Defensin transcript was also present in early-stage eggs (less than 48 h old), late-stage eggs (approximately 2 weeks old), larvae and nymphs of A. americanum and I. scapularis, both of which are vector-competent for Borrelia spirochetes. SN - 0269-283X UR - https://www.unboundmedicine.com/medline/citation/17550433/Tissue_and_life_stage_distribution_of_a_defensin_gene_in_the_Lone_Star_tick_Amblyomma_americanum_ L2 - https://doi.org/10.1111/j.1365-2915.2007.00682.x DB - PRIME DP - Unbound Medicine ER -