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Effects of the flavonoids kaempferol and fisetin on thermotolerance, oxidative stress and FoxO transcription factor DAF-16 in the model organism Caenorhabditis elegans.
Arch Toxicol 2007; 81(12):849-58AT

Abstract

Flavonoids present in many herbal edibles possess a remarkable spectrum of biochemical and pharmacological actions and they are assumed to exert beneficial effects to human health. Although the precise biological mechanisms of their action has not been elucidated yet many of the protective properties of flavonoids are attributed to their antioxidative activity since oxidative stress is regarded as a main factor in the pathophysiology of various diseases and ageing. Oxidative stress results from excessive generation of reactive oxygen species (ROS) or diminished antioxidative defence and thus antioxidants are able to counteract such situations. We used the multicellular model organism Caenorhabditis elegans that is conserved in molecular and cellular pathways to mammals to examine the effects of the flavonoids kaempferol and fisetin with respect to their protective action in individual living worms. Both flavonoids increased the survival of C. elegans, reduced the intracellular ROS accumulation at lethal thermal stress, and diminished the extent of induced oxidative stress with kaempferol having a stronger impact. Kaempferol but not fisetin attenuated the accumulation of the ageing marker lipofuscin suggesting a life prolonging activity of this flavonoid. In addition to these effects that may be attributed to their antioxidative potential kaempferol and fisetin caused a translocation of the C. elegans FoxO transcription factor DAF-16 from the cytosol to the nucleus indicating a modulatory influence of both flavonoids on signalling cascade(s).

Authors+Show Affiliations

Institute of Toxicology, Heinrich-Heine University, PO Box 101007, 40001 Düsseldorf, Germany. kampkoet@uni-duesseldorf.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17551714

Citation

Kampkötter, Andreas, et al. "Effects of the Flavonoids Kaempferol and Fisetin On Thermotolerance, Oxidative Stress and FoxO Transcription Factor DAF-16 in the Model Organism Caenorhabditis Elegans." Archives of Toxicology, vol. 81, no. 12, 2007, pp. 849-58.
Kampkötter A, Gombitang Nkwonkam C, Zurawski RF, et al. Effects of the flavonoids kaempferol and fisetin on thermotolerance, oxidative stress and FoxO transcription factor DAF-16 in the model organism Caenorhabditis elegans. Arch Toxicol. 2007;81(12):849-58.
Kampkötter, A., Gombitang Nkwonkam, C., Zurawski, R. F., Timpel, C., Chovolou, Y., Wätjen, W., & Kahl, R. (2007). Effects of the flavonoids kaempferol and fisetin on thermotolerance, oxidative stress and FoxO transcription factor DAF-16 in the model organism Caenorhabditis elegans. Archives of Toxicology, 81(12), pp. 849-58.
Kampkötter A, et al. Effects of the Flavonoids Kaempferol and Fisetin On Thermotolerance, Oxidative Stress and FoxO Transcription Factor DAF-16 in the Model Organism Caenorhabditis Elegans. Arch Toxicol. 2007;81(12):849-58. PubMed PMID: 17551714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of the flavonoids kaempferol and fisetin on thermotolerance, oxidative stress and FoxO transcription factor DAF-16 in the model organism Caenorhabditis elegans. AU - Kampkötter,Andreas, AU - Gombitang Nkwonkam,Christiane, AU - Zurawski,Ruben Felix, AU - Timpel,Claudia, AU - Chovolou,Yvonni, AU - Wätjen,Wim, AU - Kahl,Regine, Y1 - 2007/06/06/ PY - 2007/04/12/received PY - 2007/05/08/accepted PY - 2007/6/7/pubmed PY - 2008/5/13/medline PY - 2007/6/7/entrez SP - 849 EP - 58 JF - Archives of toxicology JO - Arch. Toxicol. VL - 81 IS - 12 N2 - Flavonoids present in many herbal edibles possess a remarkable spectrum of biochemical and pharmacological actions and they are assumed to exert beneficial effects to human health. Although the precise biological mechanisms of their action has not been elucidated yet many of the protective properties of flavonoids are attributed to their antioxidative activity since oxidative stress is regarded as a main factor in the pathophysiology of various diseases and ageing. Oxidative stress results from excessive generation of reactive oxygen species (ROS) or diminished antioxidative defence and thus antioxidants are able to counteract such situations. We used the multicellular model organism Caenorhabditis elegans that is conserved in molecular and cellular pathways to mammals to examine the effects of the flavonoids kaempferol and fisetin with respect to their protective action in individual living worms. Both flavonoids increased the survival of C. elegans, reduced the intracellular ROS accumulation at lethal thermal stress, and diminished the extent of induced oxidative stress with kaempferol having a stronger impact. Kaempferol but not fisetin attenuated the accumulation of the ageing marker lipofuscin suggesting a life prolonging activity of this flavonoid. In addition to these effects that may be attributed to their antioxidative potential kaempferol and fisetin caused a translocation of the C. elegans FoxO transcription factor DAF-16 from the cytosol to the nucleus indicating a modulatory influence of both flavonoids on signalling cascade(s). SN - 0340-5761 UR - https://www.unboundmedicine.com/medline/citation/17551714/Effects_of_the_flavonoids_kaempferol_and_fisetin_on_thermotolerance_oxidative_stress_and_FoxO_transcription_factor_DAF_16_in_the_model_organism_Caenorhabditis_elegans_ L2 - https://dx.doi.org/10.1007/s00204-007-0215-4 DB - PRIME DP - Unbound Medicine ER -