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[Effective of ginkgolides on expression of apoptosis related gene during PC12 cells glucose deprivation].
Zhongguo Zhong Yao Za Zhi. 2007 Mar; 32(6):532-5.ZZ

Abstract

OBJECTIVE

To investigate the protective effects of ginkgolides on glucose deprivation-induced apoptosis in PC12 cells and the mechanism underlying the protective effect.

METHOD

PC12 cells were treated under glucose deprivation, and the proliferation was determined by tetrazolium (MTT) assay. Furthermore, the mRNA levels of Bcl-2, Bax, c-myc were measured by Fluorescence Quantitative PCR (FQ-PCR).

RESULT

Ginkgolides could markedly inhibit the injury of glucose deprivation on the PC12 cells and increase the cell proliferation compared with the model groups (P <0.01). Ginkgolides could up-regulate Bcl-2 and down-regulate Bax and c-myc at 12 h, respectively. There were no significant differences in the Bcl-2 and Bax levels in both groups at 24 h, and ginkgolides only reduced the elevation of c-myc from 4. 32-fold to 2. 87-fold at this time.

CONCLUSION

Ginkgolides are able to protect the injured PC12 cells against cell apoptosis. During the early period of glucose deprivation, Bcl-2, Bax and c-myc were regulated to inhibit cell apoptosis by ginkgolides. After that, ginkgolides seems inhibit the apoptosis through attenuating the elevation of c-myc.

Authors+Show Affiliations

National Drug Screening Laboratory, China Pharmaceutical University, Nanjing 210038, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17552164

Citation

Sun, Li-Xin, et al. "[Effective of Ginkgolides On Expression of Apoptosis Related Gene During PC12 Cells Glucose Deprivation]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 32, no. 6, 2007, pp. 532-5.
Sun LX, Yan M, Jiang ZZ, et al. [Effective of ginkgolides on expression of apoptosis related gene during PC12 cells glucose deprivation]. Zhongguo Zhong Yao Za Zhi. 2007;32(6):532-5.
Sun, L. X., Yan, M., Jiang, Z. Z., Chen, M., & Zhang, L. Y. (2007). [Effective of ginkgolides on expression of apoptosis related gene during PC12 cells glucose deprivation]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 32(6), 532-5.
Sun LX, et al. [Effective of Ginkgolides On Expression of Apoptosis Related Gene During PC12 Cells Glucose Deprivation]. Zhongguo Zhong Yao Za Zhi. 2007;32(6):532-5. PubMed PMID: 17552164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effective of ginkgolides on expression of apoptosis related gene during PC12 cells glucose deprivation]. AU - Sun,Li-Xin, AU - Yan,Ming, AU - Jiang,Zhen-Zhou, AU - Chen,Min, AU - Zhang,Lu-Yong, PY - 2007/6/8/pubmed PY - 2008/5/23/medline PY - 2007/6/8/entrez SP - 532 EP - 5 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 32 IS - 6 N2 - OBJECTIVE: To investigate the protective effects of ginkgolides on glucose deprivation-induced apoptosis in PC12 cells and the mechanism underlying the protective effect. METHOD: PC12 cells were treated under glucose deprivation, and the proliferation was determined by tetrazolium (MTT) assay. Furthermore, the mRNA levels of Bcl-2, Bax, c-myc were measured by Fluorescence Quantitative PCR (FQ-PCR). RESULT: Ginkgolides could markedly inhibit the injury of glucose deprivation on the PC12 cells and increase the cell proliferation compared with the model groups (P <0.01). Ginkgolides could up-regulate Bcl-2 and down-regulate Bax and c-myc at 12 h, respectively. There were no significant differences in the Bcl-2 and Bax levels in both groups at 24 h, and ginkgolides only reduced the elevation of c-myc from 4. 32-fold to 2. 87-fold at this time. CONCLUSION: Ginkgolides are able to protect the injured PC12 cells against cell apoptosis. During the early period of glucose deprivation, Bcl-2, Bax and c-myc were regulated to inhibit cell apoptosis by ginkgolides. After that, ginkgolides seems inhibit the apoptosis through attenuating the elevation of c-myc. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/17552164/[Effective_of_ginkgolides_on_expression_of_apoptosis_related_gene_during_PC12_cells_glucose_deprivation]_ DB - PRIME DP - Unbound Medicine ER -