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Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol.
Vet J. 2008 Aug; 177(2):279-82.VJ

Abstract

A real-time PCR assay was exploited for monitoring the Leishmania DNA load in different tissues from 18 naturally-infected dogs before and after treatment with a combination of meglumine antimoniate (100mg/kg/day, subcutaneously) and allopurinol (10mg/kg/day, orally) for 30 days. After the combined therapy, allopurinol was continued at the same dose until the end of the observation period. Whole blood samples, lymph node aspirates, and skin biopsies were collected at the time of diagnosis, 1 month after starting therapy, and every 3 months for 2 years. In six dogs parasite load assessments continued every 6 months for a further 3 years. At each assessment, the dogs were examined for signs of disease and a clinical score was recorded. At diagnosis, the highest Leishmania DNA load was detected in lymph node aspirates. From 1-6 months post-therapy a general improvement in clinical conditions was recorded in all dogs, which correlated with a decrease in the parasite DNA load in all tested tissues, even though it was less pronounced in lymph node aspirates. In the period from 9-24 months post-therapy, a re-increase in parasite load was observed in the tissues of some dogs, concomitant with a disease relapse. The results show that the combined therapy with meglumine antimoniate and allopurinol promoted a clinical improvement which was accompanied by a reduction in the parasitic load in the blood, skin and lymph nodes but, even after long period of allopurinol administration alone, Leishmania may persist in dog tissues.

Authors+Show Affiliations

Dipartimento di Scienze Cliniche Veterinarie, Università di Napoli Federico II, Napoli, Italy. laumanna@unina.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17553711

Citation

Manna, Laura, et al. "Real-time PCR Assay in Leishmania-infected Dogs Treated With Meglumine Antimoniate and Allopurinol." Veterinary Journal (London, England : 1997), vol. 177, no. 2, 2008, pp. 279-82.
Manna L, Reale S, Vitale F, et al. Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol. Vet J. 2008;177(2):279-82.
Manna, L., Reale, S., Vitale, F., Picillo, E., Pavone, L. M., & Gravino, A. E. (2008). Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol. Veterinary Journal (London, England : 1997), 177(2), 279-82.
Manna L, et al. Real-time PCR Assay in Leishmania-infected Dogs Treated With Meglumine Antimoniate and Allopurinol. Vet J. 2008;177(2):279-82. PubMed PMID: 17553711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol. AU - Manna,Laura, AU - Reale,Stefano, AU - Vitale,Fabrizio, AU - Picillo,Esther, AU - Pavone,Luigi Michele, AU - Gravino,Angelo Elio, Y1 - 2007/06/05/ PY - 2007/01/15/received PY - 2007/04/19/revised PY - 2007/04/21/accepted PY - 2007/6/8/pubmed PY - 2008/10/29/medline PY - 2007/6/8/entrez SP - 279 EP - 82 JF - Veterinary journal (London, England : 1997) JO - Vet J VL - 177 IS - 2 N2 - A real-time PCR assay was exploited for monitoring the Leishmania DNA load in different tissues from 18 naturally-infected dogs before and after treatment with a combination of meglumine antimoniate (100mg/kg/day, subcutaneously) and allopurinol (10mg/kg/day, orally) for 30 days. After the combined therapy, allopurinol was continued at the same dose until the end of the observation period. Whole blood samples, lymph node aspirates, and skin biopsies were collected at the time of diagnosis, 1 month after starting therapy, and every 3 months for 2 years. In six dogs parasite load assessments continued every 6 months for a further 3 years. At each assessment, the dogs were examined for signs of disease and a clinical score was recorded. At diagnosis, the highest Leishmania DNA load was detected in lymph node aspirates. From 1-6 months post-therapy a general improvement in clinical conditions was recorded in all dogs, which correlated with a decrease in the parasite DNA load in all tested tissues, even though it was less pronounced in lymph node aspirates. In the period from 9-24 months post-therapy, a re-increase in parasite load was observed in the tissues of some dogs, concomitant with a disease relapse. The results show that the combined therapy with meglumine antimoniate and allopurinol promoted a clinical improvement which was accompanied by a reduction in the parasitic load in the blood, skin and lymph nodes but, even after long period of allopurinol administration alone, Leishmania may persist in dog tissues. SN - 1090-0233 UR - https://www.unboundmedicine.com/medline/citation/17553711/Real_time_PCR_assay_in_Leishmania_infected_dogs_treated_with_meglumine_antimoniate_and_allopurinol_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1090-0233(07)00159-1 DB - PRIME DP - Unbound Medicine ER -