Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins.Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 01; 63(Pt 6):532-4.AC
Abstract
PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 A resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 A. The crystal contained two PH1010 molecules in the asymmetric unit (V(M) = 2.4 A(3) Da(-1)) and had a solvent content of 48%.
Links
Pub Type(s)
Journal Article
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
17554180
Citation
Shirokane, Michio, et al. "Crystallization and Preliminary X-ray Analysis of PH1010 From Pyrococcus Horikoshii OT3, a Member of the Archaeal DUF54 Family of Proteins." Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 63, no. Pt 6, 2007, pp. 532-4.
Shirokane M, Sawano Y, Miyazono KI, et al. Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63(Pt 6):532-4.
Shirokane, M., Sawano, Y., Miyazono, K. I., Nagata, K., & Tanokura, M. (2007). Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 63(Pt 6), 532-4.
Shirokane M, et al. Crystallization and Preliminary X-ray Analysis of PH1010 From Pyrococcus Horikoshii OT3, a Member of the Archaeal DUF54 Family of Proteins. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):532-4. PubMed PMID: 17554180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins.
AU - Shirokane,Michio,
AU - Sawano,Yoriko,
AU - Miyazono,Ken Ichi,
AU - Nagata,Koji,
AU - Tanokura,Masaru,
Y1 - 2007/05/31/
PY - 2007/02/26/received
PY - 2007/05/18/accepted
PY - 2007/6/8/pubmed
PY - 2007/8/24/medline
PY - 2007/6/8/entrez
SP - 532
EP - 4
JF - Acta crystallographica. Section F, Structural biology and crystallization communications
JO - Acta Crystallogr Sect F Struct Biol Cryst Commun
VL - 63
IS - Pt 6
N2 - PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystal diffracted X-rays to 1.90 A resolution using a synchrotron-radiation source. The space group of the crystal was determined to be P2(1)2(1)2(1), with unit-cell parameters a = 46.9, b = 49.5, c = 132.7 A. The crystal contained two PH1010 molecules in the asymmetric unit (V(M) = 2.4 A(3) Da(-1)) and had a solvent content of 48%.
SN - 1744-3091
UR - https://www.unboundmedicine.com/medline/citation/17554180/Crystallization_and_preliminary_X_ray_analysis_of_PH1010_from_Pyrococcus_horikoshii_OT3_a_member_of_the_archaeal_DUF54_family_of_proteins_
L2 - http://scripts.iucr.org/cgi-bin/paper?S1744309107024487
DB - PRIME
DP - Unbound Medicine
ER -
