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Reproducible surface-enhanced Raman scattering spectra of bacteria on aggregated silver nanoparticles.
Appl Spectrosc. 2007 May; 61(5):479-85.AS

Abstract

Surface-enhanced Raman scattering (SERS) is proven to be a powerful technique for rapid identification and discrimination of microorganisms. However, due to the heterogeneous nature of the samples, the acquisition of reproducible spectra hinders the further development of the technique. In this study, we demonstrate the influence of the experimental conditions on SERS spectra. Then, we report a simple sample preparation method coupled with a light microscope attached to a Raman spectrometer to find a proper spot on the sample to acquire reproducible SERS spectra. This method utilizes the excited surface plasmons of the aggregated silver nanoparticles to visualize the spots on the sample. The samples are prepared using the concentrated silver colloidal solutions. The collection time for one spectrum is 10 s and each spectrum is a very good representative of the other spectra acquired from the same sample. The nature of the surface charge of the silver nanoparticles influences the spectral features by determining the strength of the interactions between nanoparticles and bacteria and the aggregation properties of the nanoparticles. Although increasing the colloid concentration in the sample resulted in reproducible spectra from arbitrary points on the sample, a great variation from sample to sample prepared with the different colloidal solution concentrations is observed.

Authors+Show Affiliations

Yeditepe University, Faculty of Engineering and Architecture, Genetics and Bioengineering Department, Kayisdagi, Istanbul, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17555616

Citation

Kahraman, Mehmet, et al. "Reproducible Surface-enhanced Raman Scattering Spectra of Bacteria On Aggregated Silver Nanoparticles." Applied Spectroscopy, vol. 61, no. 5, 2007, pp. 479-85.
Kahraman M, Yazici MM, Sahin F, et al. Reproducible surface-enhanced Raman scattering spectra of bacteria on aggregated silver nanoparticles. Appl Spectrosc. 2007;61(5):479-85.
Kahraman, M., Yazici, M. M., Sahin, F., Bayrak, O. F., & Culha, M. (2007). Reproducible surface-enhanced Raman scattering spectra of bacteria on aggregated silver nanoparticles. Applied Spectroscopy, 61(5), 479-85.
Kahraman M, et al. Reproducible Surface-enhanced Raman Scattering Spectra of Bacteria On Aggregated Silver Nanoparticles. Appl Spectrosc. 2007;61(5):479-85. PubMed PMID: 17555616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reproducible surface-enhanced Raman scattering spectra of bacteria on aggregated silver nanoparticles. AU - Kahraman,Mehmet, AU - Yazici,M Müge, AU - Sahin,Fikrettin, AU - Bayrak,Omer F, AU - Culha,Mustafa, PY - 2007/6/9/pubmed PY - 2007/7/12/medline PY - 2007/6/9/entrez SP - 479 EP - 85 JF - Applied spectroscopy JO - Appl Spectrosc VL - 61 IS - 5 N2 - Surface-enhanced Raman scattering (SERS) is proven to be a powerful technique for rapid identification and discrimination of microorganisms. However, due to the heterogeneous nature of the samples, the acquisition of reproducible spectra hinders the further development of the technique. In this study, we demonstrate the influence of the experimental conditions on SERS spectra. Then, we report a simple sample preparation method coupled with a light microscope attached to a Raman spectrometer to find a proper spot on the sample to acquire reproducible SERS spectra. This method utilizes the excited surface plasmons of the aggregated silver nanoparticles to visualize the spots on the sample. The samples are prepared using the concentrated silver colloidal solutions. The collection time for one spectrum is 10 s and each spectrum is a very good representative of the other spectra acquired from the same sample. The nature of the surface charge of the silver nanoparticles influences the spectral features by determining the strength of the interactions between nanoparticles and bacteria and the aggregation properties of the nanoparticles. Although increasing the colloid concentration in the sample resulted in reproducible spectra from arbitrary points on the sample, a great variation from sample to sample prepared with the different colloidal solution concentrations is observed. SN - 0003-7028 UR - https://www.unboundmedicine.com/medline/citation/17555616/Reproducible_surface_enhanced_Raman_scattering_spectra_of_bacteria_on_aggregated_silver_nanoparticles_ L2 - https://journals.sagepub.com/doi/10.1366/000370207780807731?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -