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Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the Bio-Quant direct ELISA presumptive assays for amphetamine and methamphetamine.
J Anal Toxicol 2007; 31(4):208-13JA

Abstract

The aim of this study was to evaluate the Bio-Quant Direct ELISA assays for amphetamine and methamphetamine in the routine presumptive screening of biological fluids. Standard concentration curves of the target analytes were assayed to assess sensitivity, and known concentrations of common amphetamine-type substances (ephedrine, pseudoephedrine, phentermine), designer analogues (MDA, MDMA, MDEA, MBDB, PMA, 4-MTA, 2CB), and putrefactive amines (phenylethylamine, putrescine, tryptamine, tyramine) were analyzed to determine cross-reactivity. Results of the standard curve studies show the capacity of both Direct ELISA kits to confidently detect down to 3 ng/mL interday (PBS matrix; CVs 6.3-15.5%). Cross-reactivity relative to that of 50 ng/mL preparations of the target compounds demonstrated that the Direct ELISA kit for amphetamine also detected MDA (282%), PMA (265%), 4-MTA (280%), and phentermine (61%), and the Direct ELISA for methamphetamine also assayed positive for MDMA (73%), MDEA (18%), pseudoephedrine (19%), MBDB (8%), and ephedrine (9%). Matrix studies demonstrated that both ELISA kits could be applied to screening of blood, urine, and saliva to a concentration of 6 ng/mL or lower. In conclusion, the Bio-Quant Direct ELISA kits for amphetamine and methamphetamine are fast and accurate and have demonstrated themselves to be useful tools in routine toxicological testing.

Authors+Show Affiliations

National Centre for Forensic Studies, University of Canberra, Bruce ACT 2601 Australia. Luigino.Apollonio@canberra.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

17555644

Citation

Apollonio, Luigino G., et al. "Matrix Effect and Cross-reactivity of Select Amphetamine-type Substances, Designer Analogues, and Putrefactive Amines Using the Bio-Quant Direct ELISA Presumptive Assays for Amphetamine and Methamphetamine." Journal of Analytical Toxicology, vol. 31, no. 4, 2007, pp. 208-13.
Apollonio LG, Whittall IR, Pianca DJ, et al. Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the Bio-Quant direct ELISA presumptive assays for amphetamine and methamphetamine. J Anal Toxicol. 2007;31(4):208-13.
Apollonio, L. G., Whittall, I. R., Pianca, D. J., Kyd, J. M., & Maher, W. A. (2007). Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the Bio-Quant direct ELISA presumptive assays for amphetamine and methamphetamine. Journal of Analytical Toxicology, 31(4), pp. 208-13.
Apollonio LG, et al. Matrix Effect and Cross-reactivity of Select Amphetamine-type Substances, Designer Analogues, and Putrefactive Amines Using the Bio-Quant Direct ELISA Presumptive Assays for Amphetamine and Methamphetamine. J Anal Toxicol. 2007;31(4):208-13. PubMed PMID: 17555644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the Bio-Quant direct ELISA presumptive assays for amphetamine and methamphetamine. AU - Apollonio,Luigino G, AU - Whittall,Ian R, AU - Pianca,Dennis J, AU - Kyd,Jennelle M, AU - Maher,William A, PY - 2007/6/9/pubmed PY - 2007/7/26/medline PY - 2007/6/9/entrez SP - 208 EP - 13 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 31 IS - 4 N2 - The aim of this study was to evaluate the Bio-Quant Direct ELISA assays for amphetamine and methamphetamine in the routine presumptive screening of biological fluids. Standard concentration curves of the target analytes were assayed to assess sensitivity, and known concentrations of common amphetamine-type substances (ephedrine, pseudoephedrine, phentermine), designer analogues (MDA, MDMA, MDEA, MBDB, PMA, 4-MTA, 2CB), and putrefactive amines (phenylethylamine, putrescine, tryptamine, tyramine) were analyzed to determine cross-reactivity. Results of the standard curve studies show the capacity of both Direct ELISA kits to confidently detect down to 3 ng/mL interday (PBS matrix; CVs 6.3-15.5%). Cross-reactivity relative to that of 50 ng/mL preparations of the target compounds demonstrated that the Direct ELISA kit for amphetamine also detected MDA (282%), PMA (265%), 4-MTA (280%), and phentermine (61%), and the Direct ELISA for methamphetamine also assayed positive for MDMA (73%), MDEA (18%), pseudoephedrine (19%), MBDB (8%), and ephedrine (9%). Matrix studies demonstrated that both ELISA kits could be applied to screening of blood, urine, and saliva to a concentration of 6 ng/mL or lower. In conclusion, the Bio-Quant Direct ELISA kits for amphetamine and methamphetamine are fast and accurate and have demonstrated themselves to be useful tools in routine toxicological testing. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/17555644/Matrix_effect_and_cross_reactivity_of_select_amphetamine_type_substances_designer_analogues_and_putrefactive_amines_using_the_Bio_Quant_direct_ELISA_presumptive_assays_for_amphetamine_and_methamphetamine_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/31.4.208 DB - PRIME DP - Unbound Medicine ER -