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Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase.
J Biotechnol. 2007 Jun 30; 130(3):316-9.JB

Abstract

Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using a recombinant Saccharomyces cerevisiae expressing together with a native xylose reductase from P. stipitis. The mutated XDHs performed the similar enzyme properties in S. cerevisiae cells, compared with those in vitro. The significant enhancement(s) was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.

Authors+Show Affiliations

Faculty of Engineering, Kyoto University, Kyotodaigaku-katsura, Kyoto 615-8530, Japan. irab@iae.kyoto-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17555838

Citation

Watanabe, Seiya, et al. "Ethanol Production From Xylose By Recombinant Saccharomyces Cerevisiae Expressing Protein Engineered NADP+-dependent Xylitol Dehydrogenase." Journal of Biotechnology, vol. 130, no. 3, 2007, pp. 316-9.
Watanabe S, Saleh AA, Pack SP, et al. Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase. J Biotechnol. 2007;130(3):316-9.
Watanabe, S., Saleh, A. A., Pack, S. P., Annaluru, N., Kodaki, T., & Makino, K. (2007). Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase. Journal of Biotechnology, 130(3), 316-9.
Watanabe S, et al. Ethanol Production From Xylose By Recombinant Saccharomyces Cerevisiae Expressing Protein Engineered NADP+-dependent Xylitol Dehydrogenase. J Biotechnol. 2007 Jun 30;130(3):316-9. PubMed PMID: 17555838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase. AU - Watanabe,Seiya, AU - Saleh,Ahmed Abu, AU - Pack,Seung Pil, AU - Annaluru,Narayana, AU - Kodaki,Tsutomu, AU - Makino,Keisuke, Y1 - 2007/04/29/ PY - 2007/01/22/received PY - 2007/03/28/revised PY - 2007/04/16/accepted PY - 2007/6/9/pubmed PY - 2007/8/30/medline PY - 2007/6/9/entrez SP - 316 EP - 9 JF - Journal of biotechnology JO - J Biotechnol VL - 130 IS - 3 N2 - Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using a recombinant Saccharomyces cerevisiae expressing together with a native xylose reductase from P. stipitis. The mutated XDHs performed the similar enzyme properties in S. cerevisiae cells, compared with those in vitro. The significant enhancement(s) was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared with the reference strain expressing the wild-type XDH. SN - 0168-1656 UR - https://www.unboundmedicine.com/medline/citation/17555838/Ethanol_production_from_xylose_by_recombinant_Saccharomyces_cerevisiae_expressing_protein_engineered_NADP+_dependent_xylitol_dehydrogenase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(07)00288-X DB - PRIME DP - Unbound Medicine ER -