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On-line enhancement and separation of nanoparticles using capillary electrophoresis.
J Chromatogr A. 2007 Aug 17; 1161(1-2):314-21.JC

Abstract

We describe a rapid, simple, and highly efficient capillary electrophoresis (CE)-based method for the analysis of nanoparticles (NPs). In this study, we used the reversed electrode polarity stacking mode (REPSM) of CE to assess the feasibility of enhancing the detection of Au NPs and Au/Ag NPs, optimizing parameters such as the length of time for which the REPSM was applied, the concentrations of the buffer and the sodium dodecylsulfate (SDS) surfactant, and the pH. Under the optimized on-line enhancement conditions [buffer: SDS (40 mM) and 3-cyclohexylamino-1-propanesulfonic acid (CAPS; 10 mM) at pH 10.0; applied voltage: 20 kV; REPSM applied for 24s], the detection limits of the Au NPs and Au/Ag NPs increased by ca. 30- and 140-fold, respectively. In addition, when the NPs were subjected to on-line enhancement and separation by CE using diode array detection (DAD), this approach allowed chemical characterization of the NP species. Our results suggest that such CE analyses will be useful for accelerating the rates of fabrication and characterization of future nanomaterials.

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Authors+Show Affiliations

Lin KH
Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University, Hsinchu 30013, Taiwan.
Chu TC
No affiliation info available
Liu FK
No affiliation info available

MeSH

BuffersElectrophoresis, CapillaryHydrogen-Ion ConcentrationMicroscopy, Electron, ScanningNanoparticlesSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17559859

Citation

Lin, Kuei-Hua, et al. "On-line Enhancement and Separation of Nanoparticles Using Capillary Electrophoresis." Journal of Chromatography. A, vol. 1161, no. 1-2, 2007, pp. 314-21.
Lin KH, Chu TC, Liu FK. On-line enhancement and separation of nanoparticles using capillary electrophoresis. J Chromatogr A. 2007;1161(1-2):314-21.
Lin, K. H., Chu, T. C., & Liu, F. K. (2007). On-line enhancement and separation of nanoparticles using capillary electrophoresis. Journal of Chromatography. A, 1161(1-2), 314-21.
Lin KH, Chu TC, Liu FK. On-line Enhancement and Separation of Nanoparticles Using Capillary Electrophoresis. J Chromatogr A. 2007 Aug 17;1161(1-2):314-21. PubMed PMID: 17559859.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - On-line enhancement and separation of nanoparticles using capillary electrophoresis. AU - Lin,Kuei-Hua, AU - Chu,Tieh-Chi, AU - Liu,Fu-Ken, Y1 - 2007/05/29/ PY - 2007/01/19/received PY - 2007/05/22/revised PY - 2007/05/23/accepted PY - 2007/6/15/pubmed PY - 2007/10/2/medline PY - 2007/6/15/entrez SP - 314 EP - 21 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1161 IS - 1-2 N2 - We describe a rapid, simple, and highly efficient capillary electrophoresis (CE)-based method for the analysis of nanoparticles (NPs). In this study, we used the reversed electrode polarity stacking mode (REPSM) of CE to assess the feasibility of enhancing the detection of Au NPs and Au/Ag NPs, optimizing parameters such as the length of time for which the REPSM was applied, the concentrations of the buffer and the sodium dodecylsulfate (SDS) surfactant, and the pH. Under the optimized on-line enhancement conditions [buffer: SDS (40 mM) and 3-cyclohexylamino-1-propanesulfonic acid (CAPS; 10 mM) at pH 10.0; applied voltage: 20 kV; REPSM applied for 24s], the detection limits of the Au NPs and Au/Ag NPs increased by ca. 30- and 140-fold, respectively. In addition, when the NPs were subjected to on-line enhancement and separation by CE using diode array detection (DAD), this approach allowed chemical characterization of the NP species. Our results suggest that such CE analyses will be useful for accelerating the rates of fabrication and characterization of future nanomaterials. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/17559859/On_line_enhancement_and_separation_of_nanoparticles_using_capillary_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(07)00953-3 DB - PRIME DP - Unbound Medicine ER -