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Activator protein-1 responsive to the group II metabotropic glutamate receptor subtype in association with intracellular calcium in cultured rat cortical neurons.
Neurochem Int 2007; 51(8):467-75NI

Abstract

Activation of ionotropic glutamate (Glu) receptors, such as N-methyl-d-aspartate receptors, is shown to modulate the gene transcription mediated by the transcription factor activator protein-1 (AP1) composed of Fos and Jun family proteins in the brain, while little attention has been paid to the modulation of AP1 expression by metabotropic Glu receptors (mGluRs). In cultured rat cortical neurons, where constitutive expression was seen with all groups I, II and III mGluR subtypes, a significant and selective increase was seen in the DNA binding activity of AP1 120 min after the brief exposure to the group II mGluR agonist (2S,2'R,3'R)-2-(2',3'-dicarboxycyclopropyl)glycine (DCG-IV) for 5 min. In cultured rat cortical astrocytes, by contrast, a significant increase was induced by a group I mGluR agonist, but not by either a group II or III mGluR agonist. The increase by DCG-IV was significantly prevented by a group II mGluR antagonist as well as by either an intracellular Ca(2+) chelator or a voltage-sensitive Ca(2+) channel blocker, but not by an intracellular Ca(2+) store inhibitor. Moreover, DCG-IV significantly prevented the increase of cAMP formation by forskolin in cultured neurons. Western blot analysis revealed differential expression profiles of Fos family members in neurons briefly exposed to DCG-IV and NMDA. Prior or simultaneous exposure to DCG-IV led to significant protection against neuronal cell death by NMDA. These results suggest that activation of the group II mGluR subtype would modulate the gene expression mediated by AP1 through increased intracellular Ca(2+) levels in cultured rat cortical neurons.

Authors+Show Affiliations

Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, Kanazawa University Graduate School of Natural Science and Technology, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17559977

Citation

Sugiyama, Chie, et al. "Activator Protein-1 Responsive to the Group II Metabotropic Glutamate Receptor Subtype in Association With Intracellular Calcium in Cultured Rat Cortical Neurons." Neurochemistry International, vol. 51, no. 8, 2007, pp. 467-75.
Sugiyama C, Nakamichi N, Ogura M, et al. Activator protein-1 responsive to the group II metabotropic glutamate receptor subtype in association with intracellular calcium in cultured rat cortical neurons. Neurochem Int. 2007;51(8):467-75.
Sugiyama, C., Nakamichi, N., Ogura, M., Honda, E., Maeda, S., Taniura, H., & Yoneda, Y. (2007). Activator protein-1 responsive to the group II metabotropic glutamate receptor subtype in association with intracellular calcium in cultured rat cortical neurons. Neurochemistry International, 51(8), pp. 467-75.
Sugiyama C, et al. Activator Protein-1 Responsive to the Group II Metabotropic Glutamate Receptor Subtype in Association With Intracellular Calcium in Cultured Rat Cortical Neurons. Neurochem Int. 2007;51(8):467-75. PubMed PMID: 17559977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activator protein-1 responsive to the group II metabotropic glutamate receptor subtype in association with intracellular calcium in cultured rat cortical neurons. AU - Sugiyama,Chie, AU - Nakamichi,Noritaka, AU - Ogura,Masato, AU - Honda,Eriko, AU - Maeda,Sayaka, AU - Taniura,Hideo, AU - Yoneda,Yukio, Y1 - 2007/05/10/ PY - 2007/02/08/received PY - 2007/04/12/revised PY - 2007/04/25/accepted PY - 2007/6/15/pubmed PY - 2007/12/22/medline PY - 2007/6/15/entrez SP - 467 EP - 75 JF - Neurochemistry international JO - Neurochem. Int. VL - 51 IS - 8 N2 - Activation of ionotropic glutamate (Glu) receptors, such as N-methyl-d-aspartate receptors, is shown to modulate the gene transcription mediated by the transcription factor activator protein-1 (AP1) composed of Fos and Jun family proteins in the brain, while little attention has been paid to the modulation of AP1 expression by metabotropic Glu receptors (mGluRs). In cultured rat cortical neurons, where constitutive expression was seen with all groups I, II and III mGluR subtypes, a significant and selective increase was seen in the DNA binding activity of AP1 120 min after the brief exposure to the group II mGluR agonist (2S,2'R,3'R)-2-(2',3'-dicarboxycyclopropyl)glycine (DCG-IV) for 5 min. In cultured rat cortical astrocytes, by contrast, a significant increase was induced by a group I mGluR agonist, but not by either a group II or III mGluR agonist. The increase by DCG-IV was significantly prevented by a group II mGluR antagonist as well as by either an intracellular Ca(2+) chelator or a voltage-sensitive Ca(2+) channel blocker, but not by an intracellular Ca(2+) store inhibitor. Moreover, DCG-IV significantly prevented the increase of cAMP formation by forskolin in cultured neurons. Western blot analysis revealed differential expression profiles of Fos family members in neurons briefly exposed to DCG-IV and NMDA. Prior or simultaneous exposure to DCG-IV led to significant protection against neuronal cell death by NMDA. These results suggest that activation of the group II mGluR subtype would modulate the gene expression mediated by AP1 through increased intracellular Ca(2+) levels in cultured rat cortical neurons. SN - 0197-0186 UR - https://www.unboundmedicine.com/medline/citation/17559977/Activator_protein_1_responsive_to_the_group_II_metabotropic_glutamate_receptor_subtype_in_association_with_intracellular_calcium_in_cultured_rat_cortical_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197-0186(07)00111-8 DB - PRIME DP - Unbound Medicine ER -