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Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress.
Arch Med Res. 2007 Jul; 38(5):503-11.AM

Abstract

BACKGROUND

Because hyperglycemia-induced oxidative stress may be a cause of retinopathy, this study examined the hypothesis that administration of exogenous antioxidants, stobadine (ST) and vitamin E (vitE), can restore retinal abnormalities in experimental diabetes.

METHODS

Normal and streptozotocin (STZ)-induced male Wistar rats received daily intraoral doses of ST (24.7 mg/kg) and vitE (alpha-dl-tocopherol acetate, 400-500 IU/kg) individually or in combinations for 8 months. The biochemical parameters including aldose reductase enzyme (AR) activity and lipid peroxidation (MDA), and histopathological changes such as retinal capillary basement membrane thickness (RCBMT) and vascular endothelial growth factor (VEGF) expression were evaluated.

RESULTS

A 37.99% increase in RCBMT was observed in rats after 8 months diabetes duration. The increase in RCBMT was 12.34% in diabetic rats treated with ST and 23.07% in diabetic rats treated with vitE. In diabetic rats treated with antioxidant combination, just a 4.38% increase was observed in RCBMT. The excess VEGF immunoreactivity and increased MDA and AR activity determined in diabetic retina were significantly attenuated by individual antioxidant treatments. Although both antioxidants decreased blood glucose, HbA1c, fructosamine and triglyceride levels in diabetic rats, poor glycemic control was maintained in all experimental groups during the treatment period. However, the antioxidant combination led to almost complete amelioration in retinal MDA and RCBMT in diabetic rats.

CONCLUSIONS

The ability of antioxidant combination to arrest retinal abnormalities and lipid peroxidation even in the presence of poor glycemic control might advocate the key role of direct oxidative damage and the protective action of antioxidants in retinal alterations associated with diabetic retinopathy.

Authors+Show Affiliations

Department of Ophthalmalogy, Gazi University, Besevler, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17560455

Citation

Yülek, Fatma, et al. "Effects of Stobadine and Vitamin E in Diabetes-induced Retinal Abnormalities: Involvement of Oxidative Stress." Archives of Medical Research, vol. 38, no. 5, 2007, pp. 503-11.
Yülek F, Or M, Ozoğul C, et al. Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress. Arch Med Res. 2007;38(5):503-11.
Yülek, F., Or, M., Ozoğul, C., Isik, A. C., Ari, N., Stefek, M., Bauer, V., & Karasu, C. (2007). Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress. Archives of Medical Research, 38(5), 503-11.
Yülek F, et al. Effects of Stobadine and Vitamin E in Diabetes-induced Retinal Abnormalities: Involvement of Oxidative Stress. Arch Med Res. 2007;38(5):503-11. PubMed PMID: 17560455.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of stobadine and vitamin E in diabetes-induced retinal abnormalities: involvement of oxidative stress. AU - Yülek,Fatma, AU - Or,Meral, AU - Ozoğul,Candan, AU - Isik,Asli Ceylan, AU - Ari,Nuray, AU - Stefek,Milan, AU - Bauer,Victor, AU - Karasu,Cimen, PY - 2006/11/23/received PY - 2007/02/20/accepted PY - 2007/6/15/pubmed PY - 2007/9/28/medline PY - 2007/6/15/entrez SP - 503 EP - 11 JF - Archives of medical research JO - Arch Med Res VL - 38 IS - 5 N2 - BACKGROUND: Because hyperglycemia-induced oxidative stress may be a cause of retinopathy, this study examined the hypothesis that administration of exogenous antioxidants, stobadine (ST) and vitamin E (vitE), can restore retinal abnormalities in experimental diabetes. METHODS: Normal and streptozotocin (STZ)-induced male Wistar rats received daily intraoral doses of ST (24.7 mg/kg) and vitE (alpha-dl-tocopherol acetate, 400-500 IU/kg) individually or in combinations for 8 months. The biochemical parameters including aldose reductase enzyme (AR) activity and lipid peroxidation (MDA), and histopathological changes such as retinal capillary basement membrane thickness (RCBMT) and vascular endothelial growth factor (VEGF) expression were evaluated. RESULTS: A 37.99% increase in RCBMT was observed in rats after 8 months diabetes duration. The increase in RCBMT was 12.34% in diabetic rats treated with ST and 23.07% in diabetic rats treated with vitE. In diabetic rats treated with antioxidant combination, just a 4.38% increase was observed in RCBMT. The excess VEGF immunoreactivity and increased MDA and AR activity determined in diabetic retina were significantly attenuated by individual antioxidant treatments. Although both antioxidants decreased blood glucose, HbA1c, fructosamine and triglyceride levels in diabetic rats, poor glycemic control was maintained in all experimental groups during the treatment period. However, the antioxidant combination led to almost complete amelioration in retinal MDA and RCBMT in diabetic rats. CONCLUSIONS: The ability of antioxidant combination to arrest retinal abnormalities and lipid peroxidation even in the presence of poor glycemic control might advocate the key role of direct oxidative damage and the protective action of antioxidants in retinal alterations associated with diabetic retinopathy. SN - 0188-4409 UR - https://www.unboundmedicine.com/medline/citation/17560455/Effects_of_stobadine_and_vitamin_E_in_diabetes_induced_retinal_abnormalities:_involvement_of_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0188-4409(07)00126-9 DB - PRIME DP - Unbound Medicine ER -